The preparation and properties of two new chromogenic substrates of trypsin

The synthesis and properties of l-lysine p-nitroanilide dihydrobromide and benzoyl dl-arginine p-nitroanilide hydrochloride are described. Both compounds are hydrolyzed by trypsin, the latter being a substrate at least as sensitive as benzoyl l-argininamide. Their hydrolysis can be followed conveniently by colorimetric procedures, since one of the products, p-nitroaniline, is yellow. Values of Km and k3 for their reaction with trypsin were...