Epidermal growth factor released from platelet-rich plasma promotes endothelial cell proliferation in vitro.

Published on Feb 1, 2010in Journal of Periodontal Research2.926
· DOI :10.1111/J.1600-0765.2009.01205.X
M.‐P. Bertrand‐Duchesne1
Estimated H-index: 1
(Laval University),
Daniel Grenier62
Estimated H-index: 62
(Laval University),
G. Gagnon1
Estimated H-index: 1
(Laval University)
Bertrand-Duchesne M-P, Grenier D, Gagnon G. Epidermal growth factor released from platelet-rich plasmapromotes endothelial cell proliferation in vitro. J Periodont Res 2009; doi: 10.1111/j.1600-0765.2009.01205.x. © 2009 The Authors. Journal compilation © 2009 Blackwell Munksgaard Background and Objective:  The therapeutic benefits of platelet-rich plasma (PRP) for the promotion of healing and regeneration of periodontal tissues are thought to result from enrichment in growth factors released from platelets. The aim of this study was to evaluate the effects of specific growth factors released from PRP on endothelial cell proliferation. Material and Methods:  The levels of vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in supernatants of calcium- and thrombin-activated PRP samples from five donors were quantified by enzyme-linked immunosorbent assay. Supernatants were treated with neutralizing antibodies specific to each growth factor, and the effects of these treatments on human umbilical vein endothelial cell (HUVEC) proliferation in vitro were determined. The effect of removing EGF from PRP supernatants with antibody-coated beads on HUVEC proliferation was also tested. Results:  Average concentrations of VEGF, PDGF-BB, bFGF and EGF in PRP supernatants were 189, 27,190, 39.5 and 513 pg/mL, respectively. The addition of EGF neutralizing antibodies to the PRP supernatants significantly reduced HUVEC proliferation (up to 40%), while such an inhibition was not observed following neutralization of the other growth factors. Removal of EGF from PRP supernatants by treatment with antibody-coated beads also resulted in a significant decrease in HUVEC proliferation. Recombinant EGF increased HUVEC proliferation in vitro in a dose-dependent manner. Conclusion:  This study showed that PRP supernatants are highly mitogenic for endothelial cells and provided evidence that this effect may be due, at least in part, to the presence of EGF. In vivo experiments are needed to confirm the roles of specific growth factors released from PRP in the healing of oral surgical and/or periodontal wounds.
Figures & Tables
📖 Papers frequently viewed together
2,085 Citations
2 Authors (D. Grenier, G. Gagnon)
1,449 Citations
#1Katarzyna Lysiak-Drwal (Wrocław Medical University)H-Index: 1
#2Marzena DominiakH-Index: 15
Last. Hanna GerberH-Index: 9
view all 7 authors...
INTRODUCTIONS: To obtain osseous tissue regeneration, treatment methods referred to as guided bone regeneration are used which utilize the mechanical, chemical, and biological properties of materials. MATERIAL/METHODS: Eighteen white rabbits were used. Under general anesthesia, a 5 mm in diameter defect was created transcutaneously in the femur trochanter major. The rabbits were divided into three groups depending on the type of the intraosseous defect (ID) treatment: in group I (C) the control ...
21 Citations
Background: The aim of the present randomized, double-masked, clinical trial was to compare platelet-rich plasma (PRP) combined with a demineralized freeze-dried bone allograft (DFDBA) to DFDBA mixed with a saline solution in the treatment of human intrabony defects.Methods: Sixty interproximal intrabony osseous defects in 60 healthy, non-smoking subjects diagnosed with chronic periodontitis were treated in this study. Thirty subjects each were randomly assigned to the test group (PRP + DFDBA) o...
90 CitationsSource
#1Yingfeng Shao (SYSU: Sun Yat-sen University)H-Index: 1
#2Danning HuH-Index: 1
Last. Jiaqi ChenH-Index: 1
view all 3 authors...
PURPOSE: To investigate effect of bFGF, EGF and NGF on growth of cultured human corneal endothelial cells. METHODS: Cultured human corneal endothelial cells were seeded into individual wells of 96-well tissue culture plate with the same culture media containing separately bFGF, EGF or NGF with a serial of concentrations of 0 ng/ml, 1 ng/ml, 3 ng/ml, 10 ng/ml, 30 ng/ml and 100 ng/ml and then cultured for 5 days. Then MTT method was used to detect the growth of the cells. RESULTS: The averaged OD ...
4 Citations
#1P RozmanH-Index: 1
#1Primoz RozmanH-Index: 10
Last. Z BoltaH-Index: 1
view all 2 authors...
Tissue repair begins with clot formation and platelet degranulation, which release the growth factors (GFs) necessary for wound repair. Platelet-derived GFs are biologically active substances that enhance tissue repair mechanisms such as chemotaxis, cell proliferation, angiogenesis, extracellular matrix deposition, and remodeling. This review describes the biological background of the topical therapy of wounds and soft-tissue injuries with platelet gel (PG) and PG-derived GFs as well as the succ...
150 Citations
Objectives: Aims of this controlled study were to determine the effects of activated human platelet-rich plasmas (PRPs) on early and mature bone formation in vivo, and to characterize the effect of PRP activation on growth factors release and endothelial cell division in vitro. Material and methods: PRPs were prepared from four volunteers with the platelet concentrate collector system (PCCS) system and activated with three concentrations of calcium and thrombin. Platelet-derived growth factor (P...
93 CitationsSource
#1Veela B. Mehta (The Research Institute at Nationwide Children's Hospital)H-Index: 15
#2Gail E. Besner (The Research Institute at Nationwide Children's Hospital)H-Index: 50
Objective: Heparin-binding EGF-like growth factor (HB-EGF) belongs to the epidermal growth factor (EGF) superfamily of ligands. It has been implicated as a regulator of angiogenesis. However, the mechanisms by which HB-EGF promotes angiogenesis are unknown. The goal of the present study was to define the pathways by which HB-EGF stimulates angiogenesis in endothelial cells.Methods: To characterize the angiogenic activity of HB-EGF, we treated human umbilical vein endothelial cells (HUVEC) with H...
101 CitationsSource
#1Michele M. Hickey (HHMI: Howard Hughes Medical Institute)H-Index: 8
#2M. Celeste Simon (HHMI: Howard Hughes Medical Institute)H-Index: 106
Maintenance of oxygen homeostasis is critical for the survival of multicellular organs. As a result, both invertebrates and vertebrates have developed highly specialized mechanisms to sense changes in oxygen levels and to mount adequate cellular and systemic responses to these changes. Hypoxia, or low oxygen tension, occurs in physiological situations such as during embryonic development, as well as in pathological conditions such as ischemia, wound healing, and cancer. A primary effector of the...
205 CitationsSource
#1William S Pietrzak (Biomet)H-Index: 2
#1William S. Pietrzak (Biomet)H-Index: 22
Last. Barry L. EppleyH-Index: 47
view all 2 authors...
Platelets play a central role in hemostasis and wound healing. The latter is mediated by release of secretory proteins on platelet activation, which directly or indirectly influences virtually all aspects of the wound healing cascade. Studies in basic science have shown a dose-response relationship
258 CitationsSource
#1J.-P. Fréchette (Laval University)H-Index: 1
#2I. Martineau (Laval University)H-Index: 1
Last. G. Gagnon (Laval University)H-Index: 1
view all 3 authors...
Platelet-rich plasmas (PRPs) are used in a variety of clinical applications, based on the premise that higher growth factor content should promote better healing. In this study, we have determined the effects of calcium and thrombin on the release of EGF, TGF-α, IGF-1, Ang-2 and IL-1β from PRPs, and assessed the mitogenic potential of PRP supernatants on osteoblast and endothelial cell division. ELISA assays indicate that (i) mean growth factor concentrations vary from traces (TGF-α) to 5.5 ng/m...
279 CitationsSource
#1James D. Kassolis (UMB: University of Maryland, Baltimore)H-Index: 5
#2Mark A. ReynoldsH-Index: 53
Abstract:Subantral sinus augmentation is often necessary to permit placement of endosseous implants. Recent efforts to improve wound healing have focused on autogenous sources of bioactive mediators, such as platelet-rich plasma (PRP), which offer the potential to enhance the biological activity of
109 CitationsSource
Cited By48
#1Matthias W. Laschke (Saarland University)H-Index: 52
#2Michael D. Menger (Saarland University)H-Index: 91
Tissue regeneration is crucially dependent on sufficient vascularization. In regenerative medicine, this can be effectively achieved by autologous vascularization strategies using the body's own resources. These strategies include the administration of blood-derived factor preparations, adipose tissue-based vascularization, and the in situ engineering of vascularized tissue. Due to their simplicity, the translation of these strategies into clinical practice is easier in terms of feasibility, saf...
#1Maki KusumiH-Index: 6
#2Tatsuji IhanaH-Index: 1
Last. Osamu TsutsumiH-Index: 57
view all 5 authors...
Purpose The purpose of this study was to investigate the effectiveness of intrauterine administration of platelet-rich plasma (PRP) in frozen embryo transfer (FET) cycle in Japanese patients with a thin endometrium. Method A prospective single-arm self-controlled trial was conducted in Japan. PRP administration was performed in 36 of the 39 eligible patients with a thin endometrium (≤7 mm). Hormone replacement therapy (HRT) with estrogen was performed for 2 menstrual cycles, and PRP was administ...
1 CitationsSource
#1Thou Lim (SJTU: Shanghai Jiao Tong University)H-Index: 2
#2Qian Tang (SJTU: Shanghai Jiao Tong University)H-Index: 2
Last. Changqing Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 52
view all 5 authors...
PURPOSE: This study evaluated the effect of thermo-sensitive hydroxybutyl chitosan (HBC) hydrogel loaded with human platelet lysate (hPL) on skin wound healing in rats. METHODS: hPLs were generated by freeze-thaw method of platelet-rich plasma from healthy donors. Successful grafting of hydroxybutyl group to chitosan molecular chain to obtain HBC hydrogel was confirmed by Fourier-transform infrared spectroscopy. HBC/hPL was prepared by combining 10% (v/v) hPL with HBC solution. Surface morpholog...
3 CitationsSource
#1Karina (UI: University of Indonesia)H-Index: 2
Last. Jeanne Adiwinata PawitanH-Index: 7
view all 11 authors...
Background: Platelet-rich plasma (PRP) contains pro-angiogenic growth factors including vascular endothelial growth factor (VEGF). Angiogenesis is a necessary component of wound healing in instances of diabetic foot ulcers (DFU). PRP composition varies depending on methods and donor health status. Our group has developed an improved PRP protocol for diabetes treatment. The aims of this study were to examine the levels of the pro-angiogenic factor VEGF in these patient populations with and withou...
6 CitationsSource
#1Yoshihiro Sowa (Kyoto Prefectural University of Medicine)H-Index: 31
#2Tsunao Kishida (Kyoto Prefectural University of Medicine)H-Index: 24
Last. Osam Mazda (Kyoto Prefectural University of Medicine)H-Index: 44
view all 6 authors...
BACKGROUND: Platelet-rich plasma contains high concentrations of growth factors that stimulate proliferation and migration of various cell types. Earlier experiments demonstrated that local platelet-rich plasma administration activates Schwann cells to improve axonal regeneration at a transected peripheral nerve lesion. However, the optimal concentration of human platelet-rich plasma for activation of human Schwann cells has not been determined, and mechanisms by which platelet-rich plasma activ...
4 CitationsSource
#1Xiaoxuan Lei (Southern Medical University)H-Index: 2
#2Pengcheng XuH-Index: 1
Last. Biao Cheng (Southern Medical University)H-Index: 4
view all 3 authors...
Background In recent years, platelet-rich plasma (PRP) has been widely applied in orthopedics, maxillofacial surgery, burns, and plastic surgery, especially in facial rejuvenation. Research is ongoing into new indications and mechanisms of PRP to promote its wider, safer, and more effective use in the clinic. This article reviews the possible mechanisms of PRP in facial rejuvenation and related research. It is expected that the application of PRP in this field will increase.
9 CitationsSource
#1Yajie Chang (SYSU: Sun Yat-sen University)H-Index: 5
#2Jingjie LiH-Index: 1
Last. Xiaoyan LiangH-Index: 1
view all 6 authors...
BACKGROUND: Adequate thickness of the endometrium has been well recognized as a critical factor for embryo implantation. This was a prospective cohort study to investigate the benefits of platelet-rich plasma (PRP) for women with thin endometrium who received frozen embryo transfer (FET) program in a larger number of patients and explore the underlying mechanism. METHODS: In this study, we investigated the effects of PRP in women with thin endometrium in FET program. 64 patients with thin endome...
21 CitationsSource
#1Ting Yuan (University of Pittsburgh)H-Index: 5
#2Changqing ZhangH-Index: 52
Last. James H.-C. Wang (University of Pittsburgh)H-Index: 60
view all 3 authors...
Tendon and ligament injuries (TLI) commonly occur in athletes and non-athletes alike, and remarkably debilitate patients’ athletic and personal abilities. Current clinical treatments, such as reconstruction surgeries, do not adequately heal these injuries and often result in the formation of scar tissue that is prone to re-injury. Platelet-rich plasma (PRP) is a widely used alternative option that is also safe because of its autologous nature. PRP contains a number of growth factors that are res...
49 CitationsSource
Last. Marta Ayala AvilaH-Index: 4
view all 10 authors...
Abstract Two phase I clinical trials were conducted to evaluate, among other parameters, the humoral response elicited by a vascular endothelial growth factor (VEGF)-based therapeutic vaccine in cancer patients with advanced solid tumors. VEGF reduction was studied using an indirect methodology named as "Platelet VEGF". This methodology is based on the estimation of VEGF within platelets by subtracting the plasma VEGF level from the serum level and dividing this by the platelet count, and then t...
2 CitationsSource
#1Marco Presta (University of Brescia)H-Index: 82
#2Eleonora Foglio (University of Brescia)H-Index: 11
Last. Roberto Ronca (University of Brescia)H-Index: 28
view all 4 authors...
Angiogenesis, the process of new blood vessel formation from pre-existing ones, plays a key role in various physiological and pathological conditions. Alteration of the angiogenic balance, consequent to the deranged production of angiogenic growth factors and/or natural angiogenic inhibitors, is responsible for angiogenesis-dependent diseases, including cancer. Fibroblast growth factor-2 (FGF2) represents the prototypic member of the FGF family, able to induce a complex “angiogenic phenotype” in...
22 CitationsSource