Peroxidase-like activity of uncoupled cytochrome P450

The NADPH-dependent consumption of O2 by cytochrome P450 BM3 was stimulated by either laurate or perfluorolaurate, but the NADPH/O2 molar consumption ratios were approximately 1 and 2, respectively, indicating that perfluorolaurate does not become oxygenated by BM3 and oxygen undergoes full reduction to water. The nature of this catalytic cycle uncoupled to hydroxylation was explored using bilirubin as a molecular probe. During uncoupling with...