Expression, purification and catalytic activity of Lupinus luteus asparagine β‐amidohydrolase and its Escherichia coli homolog

We describe the expression, purification, and biochemical characterization of two homologous enzymes, with amidohydrolase activities, of plant ( Lupinus luteus potassium‐independent asparaginase, LlA) and bacterial ( Escherichia coli , ybi K/ spt/iaa A gene product, EcAIII) origin. Both enzymes were expressed in E. coli cells, with (LlA) or without (EcAIII) a His‐tag sequence. The proteins were purified, yielding 6 or 30 mg·L −1 of culture,...