Effect of short-term administration of glucagon on gene expression of the insulin receptor in primary cultured calf hepatocytes.

Published on Jan 1, 2012in Pakistan Veterinary Journal1.318
Zhang Zhang2
Estimated H-index: 2
,
Xinwei Li34
Estimated H-index: 34
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Abstract
馃摉 Papers frequently viewed together
References15
Newest
#1Zhigang Zhang (NEAU: Northeast Agricultural University)H-Index: 25
#2Xiaobing LiH-Index: 23
Last. Xinglin ZhuH-Index: 3
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The major role of insulin and the insulin receptor (InsR) in the liver is to mediate glucose uptake into hepatocytes to synthesize glycogen and to maintain blood glucose homeostasis. In this study, we
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#1Guo-Min Liu (JLU: Jilin University)H-Index: 4
#3Jiangbin Wang (JLU: Jilin University)H-Index: 6
Last. Xinglin Zhu (JLU: Jilin University)H-Index: 3
view all 6 authors...
Summary The aim of the present study was to compare insulin receptor (IR) gene expression in normal bovine liver ( n =聽7) with samples of liver from cows in the perinatal period with ketosis ( n =聽7) and cows with fatty liver ( n =聽7). Gene expression was determined by internally controlled reverse transcriptase polymerase chain reaction (RT-PCR). The expression of IR mRNA in the liver of ketotic dairy cows was higher than in cows with fatty liver, but in both disease groups the expression was s...
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#1Eric D. BerglundH-Index: 30
#2Li Kang (Vandy: Vanderbilt University)H-Index: 20
Last. David H. Wasserman (Vandy: Vanderbilt University)H-Index: 77
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Hepatic glucagon action increases in response to accelerated metabolic demands and is associated with increased whole body substrate availability, including circulating lipids. The hypothesis that increases in hepatic glucagon action stimulate AMP-activated protein kinase (AMPK) signaling and peroxisome proliferator-activated receptor-伪 (PPAR伪) and fibroblast growth factor 21 (FGF21) expression in a manner modulated by fatty acids was tested in vivo. Wild-type (gcgr+/+) and glucagon receptor-nul...
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#1G. Bobe (Iowa State University)H-Index: 16
#2J.C. Velez (Purdue University)H-Index: 4
Last. Shawn S. Donkin (Purdue University)H-Index: 34
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Exogenous glucagon increases hepatic glucose synthesis in part by increasing hepatic extraction of amino acids from blood for conversion to glucose. To examine the role of glucagon in orchestrating gene expression of gluconeogenic and ureagenic enzymes, we determined the mRNA concentrations of key hepatic ureagenic and gluconeogenic enzymes at d 11, 15, and 22 postpartum in multiparous Holstein cows that received 0 or 5 mg of glucagon in 60 mL of saline by subcutaneous injection every 8 h for 14...
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#1Christine Longuet (U of T: University of Toronto)H-Index: 13
#2Elaine M. Sinclair (U of T: University of Toronto)H-Index: 3
Last. Daniel J. Drucker (U of T: University of Toronto)H-Index: 143
view all 7 authors...
Summary Glucagon receptor (Gcgr) signaling maintains hepatic glucose production during the fasting state; however, the importance of the Gcgr for lipid metabolism is unclear. We show here that fasted Gcgr鈭/鈭 mice exhibit a significant increase in hepatic triglyceride secretion and fasting increases fatty acid oxidation (FAO) in wild-type (WT) but not in Gcgr鈭/鈭 mice. Moreover fasting upregulated the expression of FAO-related hepatic mRNA transcripts in Gcgr+/+ but not in Gcgr鈭/鈭 mice. Exogenous ...
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#1Mohamed Osman (Iowa State University)H-Index: 3
#2Portia Allen (Iowa State University)H-Index: 4
Last. Donald C. Beitz (Iowa State University)H-Index: 60
view all 7 authors...
Abstract We examined the long-term effects of daily subcutaneous injections of 15mg of glucagon during the first 14 d postpartum with or without coadministration of 400mL of pure glycerol orally on blood metabolites and hormones and liver composition of Holstein dairy cows during early lactation. Fourteen multiparous cows with body condition score of 鈮3.5 points (1鈥5 point scale) were assigned randomly to one of 4 treatment groups鈥攕aline, glucagon, glycerol, or glucagon plus glycerol. Fatty live...
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#1K. L. Smith (Cornell University)H-Index: 4
#2M.R. Waldron (Cornell University)H-Index: 7
Last. Thomas R. Overton (Cornell University)H-Index: 49
view all 6 authors...
Abstract Holstein cows (n=72) entering second or later lactation were used to determine whether metabolic indices and hepatic capacities for oxidation and gluconeogenesis from propionate are affected by source of carbohydrate in the prepartum diet and chromium-l-methionine (Cr-Met) supplementation throughout the periparturient period. Cows were fed prepartum diets as total mixed rations with the concentrate portion based either on starch-based cereals [high nonfiber carbohydrate (NFC); 1.59 Mcal...
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#1H.M. HammonH-Index: 28
#2Cornelia C. MetgesH-Index: 31
Last. H茅l猫ne Lapierre (AAFC: Agriculture and Agri-Food Canada)H-Index: 50
view all 9 authors...
Abstract Feeding rumen-protected fat (RPF) is an alternative to increase energy density of the diet and therefore energy intake in dairy cows. To investigate metabolic and endocrine changes in dairy cows fed either a diet containing RPF (FD) or a control diet with an increased amount of cornstarch (SD), 3 Holstein cows (83卤1 d in milk) were fitted with catheters in the portal vein, a mesenteric artery, and 2 mesenteric veins. Cows were fed consecutively SD and FD for 3 wk, respectively. In FD, c...
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#1J.L. Klotz (UT: University of Tennessee)H-Index: 4
#2R.N. Heitmann (UT: University of Tennessee)H-Index: 9
Abstract Dairy calf weaning results in blood ketone concentrations in excess of mature rates of use and can result in excretion of ketones in urine representing a loss of energy. Lasalocid is frequently supplemented as an anticoccidial agent in calf starters; however, in mature ruminants it is known to alter molar ratios of ruminal volatile fatty acids (VFA). Effects of weaning transition and postweaning ionophore supplementation on body weight, dry matter (DM) intake, average daily gain (ADG), ...
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#1Jingyu Diao (U of T: University of Toronto)H-Index: 15
#2Zeenat AsgharH-Index: 7
Last. Michael B. WheelerH-Index: 80
view all 4 authors...
Abstract The insulin receptor (IR) and its signaling appear to be essential for insulin secretion from pancreatic 尾-cells. However, much less is known about the role of the IR in 伪-cells. To assess the role of the IR in glucagon and insulin secretion, we engineered adeno-viruses for high efficiency small interference RNA (siRNA)-IR expression in isolated mouse pancreatic islets and lentiviruses for siRNA-IR expression in pancreatic 伪- and 尾-cell lines (伪-TC6 and MIN6) with specific, long term st...
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Cited By2
Newest
#1Zhigang Zhang (NEAU: Northeast Agricultural University)H-Index: 25
#2Jianguo WangH-Index: 12
Last. Xinglin ZhuH-Index: 3
view all 9 authors...
Source
Received: Revised: Accepted: July 24, 2012 November 17, 2012 December 07, 2012 Ketosis is a major disease related with negative energy balance and immune suppression in dairy cows. The objective of this study was to examine the differences in 尾-hydroxybutyrate (BHBA), L-selectin, glucose, tumor necrosis factor-伪 (TNF-伪), non-esterified fatty acids (NEFA), and sialic acid (SA) concentrations in serum in healthy dairy cows and those with subclinical ketosis during the early lactation period. The b...
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