Acetyl-CoA carboxylase activity in cultured human fibroblasts

Acetyl-CoA carboxylase in human skin fibroblasts, grown in a lipid-poor medium, was inducible by insulin. The activity, ranging from 200 to 500 pmol/ min per mg of cytoplasmic protein in various cell lines, rose up to 2-fold in the presence of 1 mU/ml insulin after 3 days of incubation. The rise in acetyl-CoA carboxylase activity which was independent of cell population growth was also stimulated in proportion to log insulin concentration. The...