Effect of leptin on the gluconeogenesis in calf hepatocytes cultured in vitro

We have investigated the effect of leptin on gluconeogenesis in the liver. Hepatocytes were cultured and treated with 0, 2.5, 5, 10, 50, 100 ng/mL of leptin in groups I, II, III, IV, V, and VI, respectively. mRNA expression and enzyme activity of pyruvate carboxylase and phosphoenolpyruvate carboxykinase were determined by real‐time fluorescence quantitative RT‐PCR and biochemical kits, respectively. Compared with group I, mRNA expression of...