Active Transforming Growth Factor-β in Wound Repair

Transforming growth factor (TGF)-β regulates wound repair and scarring in an isoform-specific fashion. TGF-β is produced in a latent form, and its activation is a critical regulatory step controlling the bioactivity of this growth factor. To date, it has been impossible to determine latent TGF-β activation in vivo due to a lack of quantitative assays. We describe here a semiquantitative modification of the plasminogen activator...