Purification of recombinant proteins by fusion with thermally-responsive polypeptides
Abstract
Elastin-like polypeptides (ELPs) undergo a reversible, null phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase transition occurs, leading to aggregation of the polypeptide. We demonstrate a method for purification of soluble fusion proteins incorporating an ELP tag. Advantages of this method, termed inverse transition cycling, include technical simplicity, low...
Paper Details
Title
Purification of recombinant proteins by fusion with thermally-responsive polypeptides
Published Date
Nov 1, 1999
Journal
Volume
17
Issue
11
Pages
1112 - 1115
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