Studies on the Chemical Constituents Biological Activities of Mulberry Extracts

The freezed Mulberry (10 kg) was extracted with 80% EtOH, concentrated, and fractionated with a series of n-hexane, ethyl acetate, and water on a separatory funnel. A portion of ethyl acetate soluble (22 g) was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents. The isolated compounds were identified by cellulose TLC, -,-NMR, FAB, and EI-MS. Quercetin-3-O--D-glucopyranoside (Compound I), protocatechuic acid...