High-performance liquid chromatographic assay of NG-monomethyl-l-arginine, NG,NG-dimethyl-l-arginine, and NG,NG′-dimethyl-l-arginine using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent reagent

NG-Monomethyl-l-arginine (l-NMMA), NG,NG-dimethyl-l-arginine (ADMA), and NG,NG′-dimethyl-l-arginine (SDMA) are emerging cardiovascular risk factors. A high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of l-NMMA, ADMA and SDMA is described. The assay employed 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a fluorescent derivatization reagent. After solid phase extraction with...