Insight into Reepithelialization: How Do Mesenchymal Stem Cells Perform?

Published on Jan 1, 2016in Stem Cells International3.869
· DOI :10.1155/2016/6120173
Deyun Chen8
Estimated H-index: 8
(Chinese PLA General Hospital),
Haojie Hao23
Estimated H-index: 23
(Chinese PLA General Hospital)
+ 1 AuthorsWeidong Han50
Estimated H-index: 50
(Chinese PLA General Hospital)
Wound reepithelialization is a cooperative multifactorial process dominated by keratinocyte migration, proliferation, and differentiation that restores the intact epidermal barrier to prevent infection and excessive moisture loss. However, in wounds that exhibit impaired wound healing, such as chronic nonhealing wounds or hypertrophic scars, the reepithelialization process has failed. Thus, it is necessary to explore a suitable way to mitigate these abnormalities to promote reepithelialization and achieve wound healing. Mesenchymal stem cells (MSCs) have the capacity for self-renewal as well as potential multipotency. These cells play important roles in many biological processes, including anti-inflammation, cell migration, proliferation, and differentiation, and signal pathway activation or inhibition. The mechanism of the involvement of MSCs in reepithelialization is still not fully understood. An abundance of evidence has shown that MSCs participate in reepithelialization by inhibiting excessive inflammatory responses, secreting important factors, differentiating into multiple skin cell types, and recruiting other host cells. This review describes the evidence for the roles that MSCs appear to play in the reepithelialization process.
📖 Papers frequently viewed together
1,013 Citations
20076.02Stem Cells
4 Authors (Yaojiong Wu, ..., Edward E. Tredget)
1,605 Citations
34 Citations
#1Chui-Yee Fong (NUS: National University of Singapore)H-Index: 29
#2Kimberley Tam (NUS: National University of Singapore)H-Index: 6
Last. Ariff Bongso (NUS: National University of Singapore)H-Index: 57
view all 10 authors...
Wound healing is a major problem in diabetic patients and current treatments have met with limited success. We evaluated the treatment of excisional and diabetic wounds using a stem cell isolated from the human umbilical cord Wharton's jelly (hWJSC) that shares unique properties with embryonic and adult mesenchymal stem cells. hWJSCs are non-controversial, available in abundance, hypo-immunogenic, non-tumorigenic, differentiate into keratinocytes, and secrete important molecules for tissue repai...
33 CitationsSource
#1Jin Hao (Sichuan University)H-Index: 8
#2Yueling Zhang (Sichuan University)H-Index: 7
Last. Zhihe Zhao (Sichuan University)H-Index: 31
view all 7 authors...
Abstract Bone marrow-derived mesenchymal stem and stromal cells (MSCs) are promising candidates for cell-based therapies in diverse conditions including tissue engineering. Advancement of these therapies relies on the ability to direct MSCs toward specific cell phenotypes. Despite identification of applied forces that affect self-maintenance, proliferation, and differentiation of MSCs, mechanisms underlying the integration of mechanically induced signaling cascades and interpretation of mechanic...
95 CitationsSource
#1M. Isakson (Cork University Hospital)H-Index: 1
#2C. de Blacam (Cork University Hospital)H-Index: 2
Last. A.J.P. Clover (Cork University Hospital)H-Index: 13
view all 5 authors...
Human skin is a remarkable organ that sustains insult and injury throughout life. The ability of skin to expeditiously repair wounds is paramount to survival. With an aging global population, coupled with a rise in the prevalence of conditions such as diabetes, chronic wounds represent a significant biomedical burden. Mesenchymal stem cells (MSC), a progenitor cell population of the mesoderm lineage, have been shown to be significant mediators in inflammatory environments. Preclinical studies of...
108 CitationsSource
#1Venkat Raghavan Krishnaswamy (CLRI: Central Leather Research Institute)H-Index: 11
#2Purna Sai Korrapati (CLRI: Central Leather Research Institute)H-Index: 21
Role of Dermatopontin in re-epithelialization: Implications on keratinocyte migration and proliferation
20 CitationsSource
#1Catherine L. Ward (DA: United States Department of the Army)H-Index: 21
#2Carlos J. Sanchez (DA: United States Department of the Army)H-Index: 21
Last. Joseph C. Wenke (DA: United States Department of the Army)H-Index: 57
view all 8 authors...
Background Chronic, non-healing wounds are often characterized by the persistence of bacteria within biofilms - aggregations of cells encased within a self-produced polysaccharide matrix. Biofilm bacteria exhibit unique characteristics from planktonic, or culture-grown, bacterial phenotype, including diminished responses to antimicrobial therapy and persistence against host immune responses. Mesenchymal stromal cells (MSCs) are host cells characterized by their multifunctional ability to undergo...
34 CitationsSource
#1Hui Wang (Anhui Medical University)H-Index: 1
#2Zhao Chen (Anhui Medical University)H-Index: 1
Last. Yue-Ling Tang (Anhui Medical University)H-Index: 1
view all 5 authors...
Abstract Hypertrophic scars are characterized by excessive fibrosis and extracellular matrix (ECM) deposition and can be functionally and cosmetically problematic; however, there are few satisfactory treatments for controlling hypertrophic scars. The inflammatory cells and cytokines involved in excessive inflammation during wound healing facilitate fibroblast proliferation and collagen deposition, leading to pathologic scar formation. TSG-6 exhibits anti-inflammatory activity. This study examine...
22 CitationsSource
#1Qi Ye (Fujian Agriculture and Forestry University)H-Index: 5
#2Su-Juan WangH-Index: 10
Last. Hong ZhangH-Index: 58
view all 6 authors...
Hypertrophic scar is a complication of wound healing and has a high recurrence rate which can lead to significant abnormity in aesthetics and functions. To date, no ideal treatment method has been established. Meanwhile, the underlying mechanism of hypertrophic scarring has not been clearly defined. Although a large amount of scientific research has been reported on the use of medicinal plants as a natural source of treatment for hypertrophic scarring, it is currently scattered across a wide ran...
9 CitationsSource
#1Meirong Li (Chinese PLA General Hospital)H-Index: 10
#2Yali Zhao (Chinese PLA General Hospital)H-Index: 23
Last. Xiaobing Fu (Chinese PLA General Hospital)H-Index: 29
view all 9 authors...
The impairment of wound healing in diabetic patients is an important clinical problem. Proper keratinocyte migration and proliferation are the crucial steps during reepithelialization, and these steps may be impaired in diabetes mellitus (DM) due to hyperglycemia and chronic inflammation in wound site. In this study, we explored the effects of diabetes-like microenvironment with high glucose (HG) and intense inflammation on the migration and proliferation of keratinocytes in vitro. We found that...
26 CitationsSource
#1Deyun Chen (Chinese PLA General Hospital)H-Index: 8
#2Haojie Hao (Chinese PLA General Hospital)H-Index: 23
Last. Xiaobing Fu (Chinese PLA General Hospital)H-Index: 22
view all 10 authors...
Human umbilical cord–derived mesenchymal stem cells (UC-MSCs) are multipotent, primitive, and have been widely used for skin tissue engineering. Their transdifferentiation is determined by the local microenvironment. In this study, we investigated the potential epidermal differentiation of UC-MSCs and the formation of epidermis substitutes in a 3-dimensional (3D) microenvironment, which was fabricated by UC-MSCs embedded into collagen–chitosan scaffolds (CCSs) combined with an air–liquid interfa...
11 CitationsSource
#1Mihnea ZdrengheaH-Index: 13
#2Heidi Makrinioti (Medical Research Council)H-Index: 12
Last. Luminita A. StanciuH-Index: 34
view all 5 authors...
Activation through different signaling pathways results in two functionally different types of macrophages, the pro-inflammatory (M1) and the anti-inflammatory (M2). The polarization of macrophages toward the pro-inflammatory M1 phenotype is considered to be critical for efficient antiviral immune responses in the lung. Among the various cell types that are present in the asthmatic airways, macrophages have emerged as significant participants in disease pathogenesis, because of their activation ...
27 CitationsSource
Cited By19
#1Luca Melotti (UNIPD: University of Padua)H-Index: 12
#2Tiziana Martinello (University of Bari)H-Index: 1
Last. Marco Vincenzo Patruno (UNIPD: University of Padua)H-Index: 28
view all 11 authors...
Abstract Skin wound healing may sometimes lead to open sores that persist for long periods and expensive hospitalization is needed. Among different kinds of therapeutic innovative approaches, mesenchymal stem cells (MSCs) and low-temperature atmospheric pressure cold plasma (ionized gas) have been recently tested to improve this regenerative process. To optimize wound healing the present study intended to combine, for the first time, these two novel approaches in a large size animal wound healin...
#1Naresh Kumar Rajendran (UJ: University of Johannesburg)H-Index: 8
#2Nicolette N. Houreld (UJ: University of Johannesburg)H-Index: 24
Last. Heidi Abrahamse (UJ: University of Johannesburg)H-Index: 35
view all 3 authors...
Increasing evidence suggests that adipose-derived stem cells (ADSCs) serve as a therapeutic approach for wound healing. The aim of this study was to determine the effect of photobiomodulation (PBM) on antioxidant enzymes in ADSCs. Four ADSC cell models, namely, normal, wounded, diabetic, and diabetic wounded, were irradiated with 660 nm (fluence of 5 J/cm2 and power density of 11.2 mW/cm2) or 830 nm (fluence of 5 J/cm2 and power density of 10.3 mW/cm2). Nonirradiated cells served as controls. Ce...
#2Joonghoon Park (SNU: Seoul National University)H-Index: 12
Last. Tae Min Kim (SNU: Seoul National University)H-Index: 51
view all 3 authors...
Skin is vulnerable to various external insults such as burn, severe injury, or inflammation, which necessitates a better strategy for wound repair. Mesenchymal stem cells (MSCs) can self-renew and differentiate into various supporting tissues including cartilage, bone, muscle, and adipose tissue. Along with their unique multipotent capacity, they secrete various paracrine mediators such as growth factors, cytokines, and membrane-enclosed particles called extracellular vesicles (EVs). Herein, we ...
#1Gurjot KaurH-Index: 7
#2Poonam NegiH-Index: 14
Last. Vijay Kumar KapoorH-Index: 3
view all 3 authors...
Abstract Nutraceuticals are considered as a safe alternative to allopathic medicine and therefore are widely used for wound healing as well as part of antiaging cosmetics. In this chapter, we describe the anatomy and physiology of skin followed by the current understanding of the wound healing process. Finally, we describe some well-known nutraceuticals that have shown wound healing potential and antiaging properties on the skin. As skin nutraceuticals are an upcoming market, knowledge about phy...
#2Jin-De Hou (NDMC: National Defense Medical Center)
Last. Chian-Ze Peng (NDMC: National Defense Medical Center)H-Index: 1
view all 3 authors...
AimsTo date, there is no distinct principle determining whether to use irrigation under negative-pressure wound therapy (NPWT). We developed a new economical device to manage difficult wounds, empl...
#1Ha Bin Kim (Hoseo University)H-Index: 2
#2Kyung Jong Won (Konkuk University)H-Index: 9
Last. Hwan Myung Lee (Hoseo University)H-Index: 17
view all 5 authors...
Paederia foetida (PF) has antidiarrheal, antidiabetic, and anti-inflammatory activities. However, its biological activities on skin remain unclear. In this study, we examined the effect of PF flower absolute (PFFA) on skin wound healing- and skin barrier-linked responses in human epidermal keratinocytes (HaCaT cells). PFFA contained 23 components and increased the proliferation and sprout outgrowth of HaCaT cells and modestly increased migration. PFFA enhanced the phosphorylation levels of extra...
#1Gabriele Carullo (University of Calabria)H-Index: 13
#2Fabio Sciubba (Sapienza University of Rome)H-Index: 18
Last. Francesca Aiello (University of Calabria)H-Index: 18
view all 10 authors...
The winemaking process produces a huge number of pomaces that generally are used for energy purposes. Further valuable applications such as health-promoting properties are still under investigation. The seeds of the white berries of Mantonico and Pecorello cv. were extracted in a Soxhlet apparatus, using n-hexane and chloroform as solvents. Extracts were characterized by NMR and GC-MS analyses. They were assayed in vitro as wound healing and anti-inflammatory agents in HaCaT and RAW 264.7 cell l...
3 CitationsSource
#1Yos Adi PrakosoH-Index: 3
#2Chylen Setiyo RiniH-Index: 2
view all 5 authors...
Background and Aim Antimicrobial resistance is a global problem caused by extensive utilization of antibiotics that promote gene resistant among bacteria, including Staphylococcus aureus. This study aimed to analyze the potential effects of celery (Apium graveolens) extract as an antioxidant and antimicrobial agent against methicillin-resistant S. aureus (MRSA), in vitro and in vivo. Materials and Methods Celery was extracted and tested against a MRSA isolate in vitro. The minimum inhibitory con...
#1Brice Magne (French Institute of Health and Medical Research)H-Index: 4
#2Marianne DedierH-Index: 1
Last. Marina Trouillas (French Institute of Health and Medical Research)H-Index: 5
view all 7 authors...
Abstract Since the 1980s, deep and extensive skin wounds and burns are treated with autologous Split-Thickness Skin Grafts, or Cultured Epidermal Autografts (CEAs) when donor sites are limited. However, the clinical use of CEAs often remains unsatisfactory due to poor engraftment rates, altered wound healing and reduced skin functionality. In the past few decades, Mesenchymal Stromal Cells (MSCs) have raised much attention due to their anti-inflammatory, pro-trophic and pro-remodeling capacities...
8 CitationsSource
#1Patricia Rousselle (University of Lyon)H-Index: 44
#2Fabienne Braye (University of Lyon)H-Index: 16
Last. Guila Dayan (University of Lyon)H-Index: 2
view all 3 authors...
Abstract Cutaneous wound healing in adult mammals is a complex multi-step process involving overlapping stages of blood clot formation, inflammation, re-epithelialization, granulation tissue formation, neovascularization, and remodelling. Re-epithelialization describes the resurfacing of a wound with new epithelium. The cellular and molecular processes involved in the initiation, maintenance, and completion of epithelialization are essential for successful wound closure. A variety of modulators ...
81 CitationsSource