Methods in Cell Biology
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#1Stavroula PetridiH-Index: 2
#2Anne-Claire JacominH-Index: 12
Last. Ioannis P. NezisH-Index: 32
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Autophagy has been described as a catabolic process in which cytoplasmic material is being recycled under various conditions of cellular stress, preventing cell damage and promoting cell survival. Drosophila has been demonstrated to provide an excellent animal model for the study of autophagy. Here we provide a detailed experimental procedure for the identification of Atg8a interactors, exploiting the iLIR database, followed by the in vitro confirmation of interactions and in situ detection of t...
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#1Giulia Cerrato (Université Paris-Saclay)
#1Giulia CerratoH-Index: 4
Last. Guido Kroemer (Institut Universitaire de France)H-Index: 254
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#1Gerasimos Anagnostopoulos (Institut Universitaire de France)
Last. Maria Chiara Maiuri (Institut Universitaire de France)
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#1Nicole M. PirozziH-Index: 2
#2Jeroen KuipersH-Index: 23
Last. Ben N G GiepmansH-Index: 46
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Abstract Traditional electron microscopy (EM) can be complemented with analytical EM to increase objective sample information enabling feature identification. Energy dispersive X-ray (EDX) imaging provides semi-quantitative elemental composition of the sample with high spatial resolution (~ 10 nm) in ultrathin sections. However, EDX imaging of biological samples is still challenging as a routine method because many elements are at the detection limit for this technique. Moreover, samples undergo...
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#1In Cho (KAIST)H-Index: 1
#2Jueun Sim (KAIST)
Last. Jae-Byum Chang (KAIST)H-Index: 14
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Abstract Visualization of the spatial distribution of biomolecules with nanoscale precision is essential to understanding the molecular mechanisms of biological phenomena and diseases. Among several state-of-the-art visualization techniques, expansion microscopy (ExM) is an attractive tool, as it can achieve sub-20-nm resolution imaging of biological specimens, even with conventional diffraction-limited microscopy. This chapter first introduces the concept of ExM and its variants and then provid...
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#1Steffen Klein (University Hospital Heidelberg)H-Index: 5
#2Moritz Wachsmuth-Melm (University Hospital Heidelberg)H-Index: 2
Last. Petr Chlanda (University Hospital Heidelberg)H-Index: 17
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In situ cryo-electron tomography of cryo-focused ion beam (cryo-FIB) milled cells enables the study of cellular organelles in unperturbed conditions and close to the molecular resolution. However, due to the crowdedness of the cellular environment, the identification of individual macromolecular complexes either on organelles or inside the cytosol in cryo-electron tomograms is challenging. Cryo-correlative light and electron microscopy (cryo-CLEM) employs a fluorescently labeled feature of inter...
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#1S. Hamad Sagheer (Thomas Jefferson University)
#1S. Hamad Sagheer (Thomas Jefferson University)
Last. Nancy J. Philp (Thomas Jefferson University)H-Index: 37
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Abstract Oral squamous cell carcinoma (OSCC) is the most common subsite of head and neck cancer, with a 5-year survival rate of only 50%. There is a pressing need for animal models that recapitulate the human disease to understand the factors driving OSCC carcinogenesis. Many laboratories have used the chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) to investigate OSCC formation. The importance of the 4NQO mouse model is that it mimics the stepwise progression observed in OSCC patients. The ...
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#1Philippe PihánH-Index: 7
#2Paula Nunes-HaslerH-Index: 4
Last. Claudio HetzH-Index: 79
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#1Christina PloumiH-Index: 2
#1Christina Ploumi (FORTH: Foundation for Research & Technology – Hellas)H-Index: 1
Last. Nektarios Tavernarakis (FORTH: Foundation for Research & Technology – Hellas)H-Index: 1
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#1Federica RoccioH-Index: 1
Last. Nicolas DupontH-Index: 7
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