Yogananda S. Markandeya
National Institute of Mental Health and Neurosciences
BiophysicsInternal medicineEndocrinologyEmbryonic stem cellRepolarizationPatch clampCellular differentiationCardiologyMembrane potentialChemistryNFATProtein kinase CProgenitor cellMyocyteCaveolin 3Ventricular myocytesCaveolaeIon channelScaffold proteinMedicineReprogrammingBiologyCell biology
Publications 27
#1Jing Zhang (UW: University of Wisconsin-Madison)H-Index: 17
#2Ran Tao (UW: University of Wisconsin-Madison)H-Index: 3
Last. Timothy J. KampH-Index: 56
view all 7 authors...
Research and therapeutic applications using human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) require robust differentiation strategies. Efforts to improve hPSC-CM differentiation have largely overlooked the role of extracellular matrix (ECM). The present study investigates the ability of defined ECM proteins to promote hPSC cardiac differentiation. Fibronectin, laminin-111, and laminin-521 enabled hPSCs to attach and expand; however, fibronectin ECM either endogenously produced or e...
#1Asish Kumar Panda (IISc: Indian Institute of Science)H-Index: 4
#2K. Ravikumar (IISc: Indian Institute of Science)H-Index: 8
Last. Bikramjit Basu (IISc: Indian Institute of Science)H-Index: 60
view all 7 authors...
Engineering cellular microenvironment on a functional platform using various biophysical cues to modulate stem cell fate has been the central theme in regenerative engineering. Among the various biophysical cues to direct stem cell differentiation, the critical role of physiologically relevant electric field (EF) stimulation was established in the recent past. The present study is the first to report the strategy to switch EF-mediated differentiation of human mesenchymal stem cells (hMSCs) betwe...
#1Syeda Zehra Haider (National Institute of Mental Health and Neurosciences)H-Index: 1
#2Nivedha Mohanraj (National Institute of Mental Health and Neurosciences)
Last. Bhupesh Mehta (National Institute of Mental Health and Neurosciences)H-Index: 9
view all 5 authors...
Abstract Mitochondrial membrane potential (Ψm) is a critical parameter that can be used to determine cellular well-being. As it is a direct measure of the cell's ATP generating capability, in recent years, this key component in cell biology has been the subject of thousands of biochemical and biophysical investigations. Membrane-permeant fluorescent dyes, like tetramethylrhodamine ethyl ester (TMRE), have been predominantly employed to monitor ΔΨm in cells. These dyes are typically lipophilic ca...
#1Ruben A Alexanian (UW: University of Wisconsin-Madison)H-Index: 1
#2Kaushiki Mahapatra (UW: University of Wisconsin-Madison)H-Index: 1
Last. Timothy J. KampH-Index: 56
view all 16 authors...
Abstract Native myocardium has limited regenerative potential post injury. Advances in lineage reprogramming have provided promising cellular sources for regenerative medicine in addition to research applications. Recently we have shown that adult mouse fibroblasts can be reprogrammed to expandable, multipotent, induced cardiac progenitor cells (iCPCs) by employing forced expression of five cardiac factors along with activation of canonical Wnt and JAK/STAT signaling. Here we aim to further char...
#1Sharmistha NaskarH-Index: 6
#2Vinay Kumaran (IISc: Indian Institute of Science)H-Index: 35
Last. Bikramjit Basu (IISc: Indian Institute of Science)H-Index: 28
view all 5 authors...
Abstract A number of bioengineering strategies, using biophysical stimulation, are being explored to guide the human mesenchymal stem cells (hMScs) into different lineages. In this context, we have limited understanding regarding the transdifferentiation of matured cells to another functional-cell type, when grown with stem cells, in a constrained cellular microenvironment under biophysical stimulation. While addressing such aspects, the present work reports the influence of the electric field (...
#1Ruben A AlexanianH-Index: 1
#2Pratik A LalitH-Index: 5
Last. Timothy J. KampH-Index: 56
view all 11 authors...
Cardiac progenitor cells hold great promise for cardiac repair due to their expandability and multipotency. Recently, we have reprogrammed fibroblasts (fibs) from transgenic mice with an Nkx2.5-YFP reporter and reverse tetracycline-controlled transactivator (rtTA) into induced cardiac progenitor cells (iCPCs). The purpose of this study was to advance the clinical applicability of iCPCs by generating a reporterless reprogramming protocol, assessing neoplastic risk, and determining iCPC capacity f...
#1Yogananda S. Markandeya (UW: University of Wisconsin-Madison)H-Index: 10
#2Tadashi TsubouchiH-Index: 1
Last. Ravi C. Balijepalli (UW: University of Wisconsin-Madison)H-Index: 22
view all 6 authors...
Background Lamin A and C are nuclear filament proteins encoded by the LMNA gene. Mutations in the LMNA gene cause many congenital diseases known as laminopathies, including Emery–Dreifuss muscular dystrophy, Hutchinson–Gilford progeria syndrome, and familial dilated cardiomyopathy (DCM) with conduction disease. A missense mutation (N195K) in the A-type lamins results in familial DCM and sudden arrhythmic death. Objective The purpose of this study was to investigate the ion current mechanism of a...
#1Daryl O. Nelson (UW: University of Wisconsin-Madison)H-Index: 3
#2Pratik A Lalit (UW: University of Wisconsin-Madison)H-Index: 5
Last. Gary E. Lyons (UW: University of Wisconsin-Madison)H-Index: 47
view all 13 authors...
Rationale: While much progress has been made in the resolution of the cellular hierarchy underlying cardiogenesis, our understanding of chamber-specific myocardium differentiation remains incomplete. Objective: To better understand ventricular myocardium differentiation, we targeted the ventricle-specific gene, Irx4, in mouse embryonic stem cells to generate a reporter cell line. Methods and Results: Using an antibiotic-selection approach, we purified Irx4+ cells in vitro from differentiating em...
#1Ravi Vaidyanathan (UW: University of Wisconsin-Madison)H-Index: 16
#2Yogananda S. Markandeya (UW: University of Wisconsin-Madison)H-Index: 10
Last. Lee L. Eckhardt (UW: University of Wisconsin-Madison)H-Index: 17
view all 6 authors...
Ik1-enhanced induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) more closely represent the cell size, structure, multinucleation, electrophysiology, and calcium handling properties of adult human cardiomyocytes. These IK1-enhanced iPS-CMs will permit advanced studies of arrhythmia mechanisms such as rotor and reentry generation in plated iPS-CMs or drug safety testing due to the mature cellular phenotype.
#1Pratik A Lalit (UW: University of Wisconsin-Madison)H-Index: 5
#2Max R. Salick (UW: University of Wisconsin-Madison)H-Index: 25
Last. Timothy J. KampH-Index: 56
view all 21 authors...
Summary Several studies have reported reprogramming of fibroblasts into induced cardiomyocytes; however, reprogramming into proliferative induced cardiac progenitor cells (iCPCs) remains to be accomplished. Here we report that a combination of 11 or 5 cardiac factors along with canonical Wnt and JAK/STAT signaling reprogrammed adult mouse cardiac, lung, and tail tip fibroblasts into iCPCs. The iCPCs were cardiac mesoderm-restricted progenitors that could be expanded extensively while maintaining...
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