Peng Lin
Boston University
PhenotypeOligonucleotideGenotypeFunction (biology)MicrobiomeChemistryStable-isotope probingBacteriaBiochemistryIn situ hybridization
Publications 2
#1Hongli NiH-Index: 1
#2Peng LinH-Index: 6
Last. Ji-Xin ChengH-Index: 83
view all 7 authors...
Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally-sensitive solid-state optical parametric oscillator (OPO) in current SRS microscope. Moreover, chemically-informative multi-window SRS imaging across C-H, C-D and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multi-window SRS ima...
#1Xiaowei Ge (BU: Boston University)
#2Fátima C. PereiraH-Index: 11
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 6 authors...
For high-throughput link of microbiome function and taxonomic identity at the single cell level, we established a stimulated Raman scattering (SRS)-fluorescence in situ hybridization (FISH) platform. SRS combined with the deuterium-based isotope probing enables chemical mapping and reveals metabolic activity of bacteria. Fluorescently tagged oligonucleotide probes identify different bacteria and are detected through two photon fluorescence (TPF) microscopy. As a proof-of-principle demonstration,...