Daniela Di Paolo
Istituto Giannina Gaslini
CancerPathologyMolecular biologyNanocarriersLiposomeChemistryApoptosisTherapeutic indexIn vitroImmunologyIn vivoNeuroblastomaDrug deliveryAngiogenesisDoxorubicinCancer researchCell growthMedicineCell cultureBiologyCancer cellPharmacology
47Publications
22H-index
1,265Citations
Publications 47
Newest
#1Müjdat Zeybel (Newcastle University)H-Index: 11
#2Saimir Luli (Newcastle University)H-Index: 8
Last. Derek A. Mann (Newcastle University)H-Index: 71
view all 18 authors...
The progression of fibrosis in chronic liver disease is dependent upon hepatic stellate cells (HSCs) transdifferentiating to a myofibroblast-like phenotype. This pivotal process is controlled by enzymes that regulate histone methylation and chromatin structure, which may be targets for developing anti-fibrotics. There is limited pre-clinical experimental support for the potential to therapeutically manipulate epigenetic regulators in fibrosis. In order to learn if epigenetic treatment can halt t...
46 CitationsSource
#1Chiara Brignole (Boston Children's Hospital)H-Index: 29
#2Patrizia Perri (Boston Children's Hospital)H-Index: 22
Last. Mirco Ponzoni (Boston Children's Hospital)H-Index: 55
view all 11 authors...
The depletion of tumor-associated macrophages (TAMs), involved in different stages of cancer development and progression, is an appealing strategy in cancer therapy. We developed novel Clodronate-containing liposomes (Clo-Lipo-DOTAP) presenting good physicochemical properties (size distribution, polidispersity index and Z-potential). In vitro, Clo-Lipo-DOTAP inhibited proliferation, reduced viability and induced apoptosis of a macrophage-like cell line in a dose- and time-dependent manner. In pr...
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#1Saimir Luli (Newcastle University)H-Index: 8
#2Daniela Di Paolo (Istituto Giannina Gaslini)H-Index: 22
Last. Fiona Oakley (Newcastle University)H-Index: 37
view all 12 authors...
Background & Aims Currently, staging of fibrosis in preclinical rodent liver fibrosis models is achieved histologically. Many animals are used at multiple time-points to assess disease progression or therapeutic responses. Hepatic myofibroblasts promote liver fibrosis therefore quantifying these cells in vivo could assess disease or predict therapeutic responses in mice. We fluorescently labelled a single chain antibody (C1-3) that binds hepatic myofibroblasts to monitor fibrogenesis in vivo . M...
10 CitationsSource
#1Francesca Piaggio (Istituto Giannina Gaslini)H-Index: 7
#2Vangelis Kondylis (University of Cologne)H-Index: 21
Last. Chiara Brignole (Istituto Giannina Gaslini)H-Index: 29
view all 18 authors...
Abstract The depletion of tumor-associated macrophages (TAMs), involved in different stages of cancer development and progression, is an appealing strategy in cancer therapy. We developed novel Clodronate-containing liposomes (Clo-Lipo-DOTAP) presenting physicochemical properties (size distribution, polydispersity index and Z-potential) suited for safe storage and injections. In vitro , Clo-Lipo-DOTAP inhibited proliferation, reduced viability and induced apoptosis of a macrophage-like cell line...
47 CitationsSource
#1Irene CossuH-Index: 6
#2Gianluca BottoniH-Index: 11
Last. Fabio PastorinoH-Index: 34
view all 25 authors...
Selective tumor targeting is expected to enhance drug delivery and to decrease toxicity, resulting in an improved therapeutic index. We have recently identified the HSYWLRS peptide sequence as a specific ligand for aggressive neuroblastoma, a childhood tumor mostly refractory to current therapies. Here we validated the specific binding of HSYWLRS to neuroblastoma cell suspensions obtained either from cell lines, animal models, or Schwannian-stroma poor, stage IV neuroblastoma patients. Binding o...
26 CitationsSource
#1Alice BartoliniH-Index: 20
#2Daniela Di PaoloH-Index: 22
Last. Serena Marchiò (UNITO: University of Turin)H-Index: 20
view all 12 authors...
Neuronal pentraxins (NPTX) and their corresponding receptors (NPTXR) have been studied as synapse-associated proteins in the nervous system, but their role in cancer is largely unknown. By applying a multidisciplinary, high-throughput proteomic approach, we have recently identified a peptide ligand motif for targeted drug delivery to neuroblastoma. Here we report the sequence similarity between this peptide and a conserved portion of the pentraxin domain that is involved in the homo- and hetero-...
12 CitationsSource
#1Guendalina Zuccari (UNIBO: University of Bologna)H-Index: 14
#2Andrea Milelli (UNIBO: University of Bologna)H-Index: 19
Last. Mirco PonzoniH-Index: 55
view all 18 authors...
Abstract Neuroblastoma is a childhood cancer with poor long-term prognosis in advanced stages. A major aim in neuroblastoma therapy is to develop targeted drug delivery systems to ameliorate drug therapeutic index and efficacy. In this study, a novel bortezomib (BTZ) liposomal formulation was set-up and characterized. Since BTZ is freely permeable across the lipidic bilayer, an amino-lactose (LM) was synthesized as complexing agent to entrap BTZ inside the internal aqueous compartment of stealth...
37 CitationsSource
#1Daniela Di PaoloH-Index: 22
#1D. Di PaoloH-Index: 5
Last. Patrizia Perri (Istituto Giannina Gaslini)H-Index: 22
view all 15 authors...
// Daniela Di Paolo 1 , D. Yang 2 , Fabio Pastorino 1 , Laura Emionite 3 , Michele Cilli 3 , Antonio Daga 4 , Elisa Destafanis 1, 6 , Annarita Di Fiore 1 , Francesca Piaggio 1 , Chiara Brignole 1 , Xiaobao Xu 2 , Chris Liang 5 , James Gibbons 5 , Mirco Ponzoni 1, * , Patrizia Perri 1, * 1 Laboratorio di Oncologia, Istituto G. Gaslini, Genoa, Italy 2 Sundia MediTech Company, Ltd., Shangai, China 3 Animal Facility, IRCCS Azienda Ospedaliera Universitaria San Martino-IST Istituto Nazionale per la R...
12 CitationsSource
#1Alice BartoliniH-Index: 20
#2Monica Loi (Istituto Giannina Gaslini)H-Index: 18
Last. Fabio Pastorino (Istituto Giannina Gaslini)H-Index: 34
view all 19 authors...
Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Introduction. The identification of peptide ligands specific for solid tumors is expected to provide targeting moieties to improve delivery and to decrease toxicity of chemotherapy. We have recently identified the peptide HSYWLRS as a specific ligand for neuroblastoma (NB), a childhood tumor mostly refractory to current therapies. Experimental procedures. The capability of peptide HSYWLRS to recognize NB cells was evaluated by...
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#1Daniela Di PaoloH-Index: 22
#1D. Di PaoloH-Index: 5
Last. Patrizia PerriH-Index: 22
view all 11 authors...
Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA The anaplastic lymphoma kinase (ALK), a tyrosine kinase receptor involved in the genesis of several human cancers, is a promising therapeutic target in Neuroblastoma (NB). Many studies on sporadic cases with advanced NB have shown ALK mutated or highly expressed independently of its genetic status (mutated, amplified, wild-type) and its pivotal role in NB growth and survival. As ALK is currently considered a master driver of N...
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