Huu Phuc Nguyen
Ruhr University Bochum
GeneCorticogenesisEpigenomeProtein–protein interactionAcetylationWnt signaling pathwayFunction (biology)CytoskeletonEmbryonic stem cellCellGene expressionCell signalingProgenitorTranscriptomeChromatin remodelingPost-transcriptional regulationChemistryProgenitor cellTranscriptional regulationLaminar organizationNeocortexCortex (anatomy)Cell sortingPeptideForebrainProtein BiochemistryRNA StabilityExosome Multienzyme Ribonuclease ComplexHistone H3 acetylationCell cycleCell specificCortical tissueCortex (botany)MorphogenesisEpigeneticsImmunostainingProteomicsMutationHigh-throughput screeningRNAComputational biologyExosome complexChromatinCell adhesionTranscription factorBiologyCell biology
6Publications
2H-index
5Citations
Publications 5
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#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
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Summary Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases. Traditional PPI assays are laborious, involving the generation of many truncated proteins. SPOT peptide assay allows high-throughput detection of domains essential for PPI by synthesizing several hundred peptides on a cellulose membrane. Here, we present a rapid SPOT peptide protocol for identify...
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#1Pauline Antonie Ulmke (GAU: University of Göttingen)H-Index: 2
#2M. Sadman Sakib (German Center for Neurodegenerative Diseases)H-Index: 6
Last. Tran Tuoc (GAU: University of Göttingen)H-Index: 16
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Summary Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (−) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2+ IPCs versus TBR2− cells revealed differences in key factors involved in chromatid ...
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#1M. Sadman Sakib (German Center for Neurodegenerative Diseases)H-Index: 6
#2Godwin Sokpor (RUB: Ruhr University Bochum)H-Index: 1
Last. Tran Tuoc (RUB: Ruhr University Bochum)H-Index: 16
view all 5 authors...
Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized ...
2 CitationsSource
#1Pauline Antonie Ulmke (GAU: University of Göttingen)H-Index: 2
#2Yuanbin Xie (GAU: University of Göttingen)H-Index: 2
Last. Tran Tuoc (GAU: University of Göttingen)H-Index: 16
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Fine-tuned gene expression is crucial for neurodevelopment. The gene expression program is tightly controlled at different levels, including RNA decay. N6-methyladenosine (m6A) methylation-mediated degradation of RNA is essential for brain development. However, m6A methylation impacts not only RNA stability, but also other RNA metabolism processes. How RNA decay contribute to brain development is largely unknown. Here, we show that Exosc10, an RNA exonuclease subunit of the RNA exosome complex, ...
1 CitationsSource