Florian Szymczak
Université libre de Bruxelles
Beta (finance)GenePattern recognition receptorInternal medicineDownregulation and upregulationEndocrinologyGene expressionMolecular biologyIsletGene knockdownChemistryPeroxisome proliferator-activated receptorImmunologyLupus erythematosusInterferonBeta cellInflammationType 2 diabetesRheumatoid arthritisRAB7ACytokineReference genesInsulitisPancreatic isletsMultiple sclerosisType 1 diabetesExtracellular matrix organizationPancreatic beta CellsRt pcr analysisLipotoxicityCancer researchCandidate geneText miningMedicineBiologyImmune systemCoefficient of variation
5Publications
2H-index
5Citations
Publications 6
Newest
#1Decio L. Eizirik (ULB: Université libre de Bruxelles)H-Index: 103
#2Florian Szymczak (ULB: Université libre de Bruxelles)H-Index: 2
Last. Frank Martin (JDRF)H-Index: 1
view all 4 authors...
Completion of the Human Genome Project enabled a novel systems- and network-level understanding of biology, but this remains to be applied for understanding the pathogenesis of type 1 diabetes (T1D). We propose that defining the key gene regulatory networks that drive β-cell dysfunction and death in T1D might enable the design of therapies that target the core disease mechanism, namely, the progressive loss of pancreatic β-cells. Indeed, many successful drugs do not directly target individual di...
Source
#1Maria Inês Alvelos (ULB: Université libre de Bruxelles)H-Index: 6
#2Florian Szymczak (ULB: Université libre de Bruxelles)H-Index: 2
Last. Decio L. EizirikH-Index: 103
view all 14 authors...
Exposure of human pancreatic beta cells to pro-inflammatory cytokines or metabolic stressors is used to model events related to type 1 and type 2 diabetes, respectively. Quantitative real-time PCR is commonly used to quantify changes in gene expression. The selection of the most adequate reference gene(s) for gene expression normalization is an important pre-requisite to obtain accurate and reliable results. There are no universally applicable reference genes, and the human beta cell expression ...
Source
#1Maria Inês Alvelos (ULB: Université libre de Bruxelles)H-Index: 6
#2Florian SzymczakH-Index: 2
Last. Mariana Igoillo-EsteveH-Index: 25
view all 14 authors...
Exposure of human pancreatic beta cells to pro-inflammatory cytokines or metabolic stressors is used to model events related to type 1 and type 2 diabetes, respectively. Quantitative real-time PCR is commonly used to quantify changes in gene expression. The selection of the most adequate reference gene(s) for gene expression normalization is an important pre-requisite to obtain accurate and reliable results. There are no universally applicable reference genes, and the human beta cell expression ...
Source
#1Florian Szymczak (ULB: Université libre de Bruxelles)H-Index: 2
#2Maikel Luis Colli (ULB: Université libre de Bruxelles)H-Index: 18
Last. Decio L. Eizirik (ULB: Université libre de Bruxelles)H-Index: 103
view all 5 authors...
Autoimmune diseases are typically studied with a focus on the immune system, and less attention is paid to responses of target tissues exposed to the immune assault. We presently evaluated, based on available RNA sequencing data, whether inflammation induces similar molecular signatures at the target tissues in type 1 diabetes, systemic lupus erythematosus, multiple sclerosis, and rheumatoid arthritis. We identified confluent signatures, many related to interferon signaling, indicating pathways ...
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#1Maikel Luis Colli (ULB: Université libre de Bruxelles)H-Index: 18
#2Florian Szymczak (ULB: Université libre de Bruxelles)H-Index: 2
Last. Decio L. Eizirik (ULB: Université libre de Bruxelles)H-Index: 103
view all 3 authors...
Type 1 diabetes (T1D) is a chronic disease caused by the selective destruction of the insulin-producing pancreatic beta cells by infiltrating immune cells. We presently evaluated the transcriptomic signature observed in beta cells in early T1D and compared it with the signatures observed following in vitro exposure of human islets to inflammatory or metabolic stresses, with the aim of identifying "footprints" of the immune assault in the target beta cells. We detected similarities between the be...
Source
#1Xiaoyan YiH-Index: 2
#2Lorella MarselliH-Index: 50
Last. Miriam CnopH-Index: 57
view all 8 authors...
Gluco- and lipotoxicity impair β cell function and survival, potentially contributing to type 2 diabetes onset and progression. Here we profiled transcriptomes of human islets in gluco/lipotoxic and type 2 diabetes conditions. Human islets from 26 nondiabetic donors were exposed to 0.5 mM palmitate (P), 22.2 mM glucose (G), P+G, 1 mM palmitate+oleate, (1:2 ratio, P+O) and P+O+G for 2 days (n=2-5/condition), followed by 4 days of washout. Glucose-stimulated insulin secretion and RNA-seq transcrip...
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