Tadashi Furukawa
Daiichi Sankyo
Circadian rhythmInternal medicineEndocrinologyChemistryEstrous cycleImmunologyPrimary and secondary antibodiesT cellKeyhole limpet hemocyaninUnspecific monooxygenaseSuprachiasmatic nucleusAdrenalectomyToxicityP450 monooxygenaseMetabolic enzymesF344 ratsGrowth hormone secretionMale ratsCytochrome P450Period (gene)MedicineBiologyImmune systemPharmacology
Publications 19
#1Ryota Kawai (Daiichi Sankyo)H-Index: 4
#2Tetsuo Aida (Daiichi Sankyo)H-Index: 6
Last. Teruo Kawada (Kyoto University)H-Index: 64
view all 8 authors...
T-cell dependent antibody response (TDAR) incorporating both primary and secondary responses to keyhole limpet hemocyanin (KLH) in canine models have not yet been fully understood. To develop a practical dog TDAR model, we characterized primary and secondary antibody responses by intravenous or intramuscular immunization of KLH twice at intervals of 8 days during a 28-day course of study. Primary immunization with KLH by both routes induced a maximum IgM response on 6 to 8 days after the treatme...
6 CitationsSource
#1Ryota KawaiH-Index: 4
#2Shiho ItoH-Index: 1
Last. Teruo Kawada (Kyoto University)H-Index: 64
view all 9 authors...
To develop a rat T-cell-dependent antibody response (TDAR) model evaluating both primary and secondary antibody responses, keyhole limpet hemocyanin (KLH) was used to immunize rats twice during a 14-day course of study, a pattern closely linked to that of a short-term general toxicity study. Female rats of four representative strains (e.g., Sprague-Dawley, Wistar, Fischer, and Lewis) were immunized twice with intravenous administrations of KLH (300 µg/rat) on Days 5 and 9 during a 14-day treatme...
18 CitationsSource
#1Jun Hirao (Daiichi Sankyo)H-Index: 4
#2Masatoshi Nishimura (Daiichi Sankyo)H-Index: 1
Last. Yuji Mori (UTokyo: University of Tokyo)H-Index: 51
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The present study was designed to fully uncover sex and circadian modulatory effects on rat liver. Hepatic transcriptome analyses were performed at 4 hr intervals of a day-night cycle using young adult male and female rats. Sexually dimorphic genes, which were identified by a cross-sex comparison of time series data, included representative sex-predominant genes such as male- or female-predominant cytochrome P450 subfamilies (Cyp2c11, Cyp2c12, Cyp2c13, and Cyp3a2), sulfotransferases, and glutath...
23 CitationsSource
#1Jun Hirao (Daiichi Sankyo)H-Index: 4
#2Noriyo Niino (Daiichi Sankyo)H-Index: 8
Last. Masugi Nishihara (UTokyo: University of Tokyo)H-Index: 40
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The secretory profile of growth hormone (GH) is sexually dimorphic in rats. In male transgenic (TG) rats expressing human GH (hGH) that we generated, the circulating levels of both hGH and endogenous GH are flattened with no male-type pulsatility. To elucidate the regulatory role of episodic GH profile on the liver, the hepatic transcriptome of male TG rats at the middle of the light and dark phases was characterized by genome-wide analyses as compared with that of male wild-type (WT) rats. Tran...
4 CitationsSource
#1Makoto Shirai (Daiichi Sankyo)H-Index: 4
#2Ken Sakurai (Daiichi Sankyo)H-Index: 5
Last. Sunao Manabe (Daiichi Sankyo)H-Index: 17
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The main focus of this study was to determine the optimal administration period concerning the toxic effects on ovarian morphological changes in the repeated dose toxicity study. In order to assess the morphological and functional changes induced in the ovary by cyclophosphamide (CP), the compound was administrated to female rats at dose levels of 0, 5, 10 and 20 mg/kg for the repeated dose toxicity study for 2 or 4 weeks, and at 0, 5, 10, and 20 mg/kg for the female fertility study from 2 weeks...
15 CitationsSource
#1Jun HiraoH-Index: 3
#2Shingo ArakawaH-Index: 11
Last. Tadashi FurukawaH-Index: 3
view all 5 authors...
Abstract Hepatic P450 monooxygenase activities, assessed by measurement of 7-alkoxycoumarin O-dealkylase (ACD) activities, show obvious daily fluctuations in male rats with high values during the dark period and low values during the light period. We have already confirmed that the ACD activities are controlled by the suprachiasmatic nucleus (SCN), which is well known as the oscillator of circadian rhythm. Recently, it is reported that circadian oscillators exist not only in the SCN but also in ...
26 CitationsSource
#1Tetsuo AidaH-Index: 6
#2Ryota KawaiH-Index: 4
Last. Tadashi FurukawaH-Index: 8
view all 4 authors...
#1Jun HiraoH-Index: 4
#2Shingo ArakawaH-Index: 11
Last. Tadashi FurukawaH-Index: 3
view all 5 authors...
There is a possibility that serious liver dysfunction rarely observed in diabetic patients given troglitazone is attributable to idiosyncratic abnormalities in liver drug-metabolism. In addition, the results of blood biochemical examinations in serious cases of liver dysfunction showed a tendency for a high level of total bilirubin(T-Bil)over a long period compared with other indicators of liver dysfunction. Thus, we focused on genetic variation of UDP-glucuronosyltransferases(UGTs)that are invo...
11 CitationsSource
#1Tadashi FurukawaH-Index: 8
#2Sunao ManabeH-Index: 17
Last. Yuji Mori (UTokyo: University of Tokyo)H-Index: 51
view all 5 authors...
Abstract Daily fluctuations have been observed in the hepatic cytochrome P450 monooxygenase activities of rodents. It was confirmed in our previous study that the P450 activities assessed by measurement of 7-alkoxycoumarin O -dealkylase (ACD) showed obvious daily fluctuations in male rats with high values during the dark period. However, there is still very little information about the daily fluctuation of P450 activities in female rats. In the first experiment of the present study, the daily fl...
18 CitationsSource