Alice Nava
Internal medicineViral loadAntigenVirologyPore-forming toxinStage (cooking)Predictive value of testsYoung adultGlutamate dehydrogenaseCoronavirusPneumoniaSeroprevalenceMycobacterium tuberculosis complexUrineTuberculosis diagnosisLiaisonTuberculosisSeroepidemiologic StudiesChemiluminescent AssaysSpecific testClostridium difficileSeverity of illness2019-20 coronavirus outbreakSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Hospital dischargeC difficileExtrapulmonary tuberculosisTiterSerologyReal-time polymerase chain reactionDigital polymerase chain reactionPathogenicityRNAMedicineBiologyMicrobiologyPandemicGastroenterologyClostridium difficile toxin A
5Publications
3H-index
60Citations
Publications 5
Newest
#1Valeria CentoH-Index: 21
#2Claudia Alteri (University of Milan)H-Index: 19
Last. Carlo Federico PernoH-Index: 76
view all 22 authors...
Objective Through a hospital-based SARS-CoV-2 molecular and serological screening, we evaluated the effectiveness of two months of lockdown and two of surveillance, in Milan, Lombardy, the first to be overwhelmed by COVID-19 pandemics during March-April 2020. Methods All subjects presenting at the major hospital of Milan from May-11 to July-5, 2020, underwent a serological screening by chemiluminescent assays. Those admitted were further tested by RT-PCR. Results The cumulative anti-N IgG seropr...
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#1Claudia AlteriH-Index: 19
#2Valeria CentoH-Index: 21
Last. Carlo Federico Perno (University of Milan)H-Index: 76
view all 24 authors...
Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of a highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises as a major clinical need. Here we evaluated the performance of a ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks to in-house ddPCR assay (targeting RdRp and host RNaseP). Samples were collected at ASST-GOM Niguarda betwe...
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#1Valeria Cento (University of Milan)H-Index: 21
#2L. Colagrossi (Boston Children's Hospital)H-Index: 5
Last. Carlo Federico Perno (University of Milan)H-Index: 76
view all 25 authors...
Source
: The real time PCR Xpert ® MTB/RIF is fundamental for rapid diagnosis in paucibacillary respiratory samples and for the detection of multidrug-resistant TB cases. This paper aimed to determine its performance on different extrapulmonary samples. We determined sensitivity, specificity, positive and negative predictive value on respiratory and non-respiratory samples collected from January 2010 to June 2014. The protocol for the Xpert ® MTB/RIF PCR suggested by Cepheid was strictly followed for a...
#2Alessandra BielliH-Index: 3
Last. Daniela CampisiH-Index: 6
view all 4 authors...
The presumptive laboratory diagnosis of Clostridium difficile infection is achieved by the means of the detection of a common antigen (glutamate dehydrogenase, GDH) in stool, then confirming the positives either by the detection of toxins A and B or by a molecular test for the detection of pathogenicity locus , encoding for the two toxins and for the binary toxin. A fully automated chemiluminescence system for the GDH antigen (LIAISON® C. difficile GDH) and for the detection of toxins A and B (L...
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