Xue Qin
Northeast Agricultural University
RecombinaseLinear rangeGeneColloidal goldComparative genomicsDetection limitTransduction (genetics)ChromogenicAptamerOligonucleotideNuclear chemistryAnimal scienceGenomeMolecular biologyInternal transcribed spacerNaked eyePesticideDoseEnzymeChemistryConjugateCombinatorial chemistryPlasmidNucleic acidQuechersRaw milkCronobacter sakazakiiCarbonyl ironHydroxynaphthol blueNanoclustersPathogenic bacteriaInfant formulaAcetochlorSalmonella entericaCronobacterMagnetic separationDipstickImmunomagnetic separationSalmonellaMultiplexExonuclease IIICronobacter speciesPositive correlationColorimetric sensorPure cultureTreatment periodDietary intakeLinear relationshipChromatographyCarbonBacteriaGeneticsEscherichia coliDNALoop-mediated isothermal amplificationRibosomal RNAPrimer (molecular biology)Multilocus sequence typingDNA extractionGenetic diversityZeta potentialBiologyFluorescenceNanocompositeHorizontal gene transferAbsorbance
10Publications
3H-index
14Citations
Publications 11
Newest
#1Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
#2Lihan Wang (NEAU: Northeast Agricultural University)H-Index: 2
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 8 authors...
Abstract null null A novel fluorescent platform of DNA-stabilized silver nanoclusters (DNA-AgNCs) has been developed based on exonuclease III (Exo III) amplification-assisted for simple and sensitive detection of Salmonella Typhimurium (S. Typhimurium). The platform was designed by using magnetic beads, aptamer, its complementary DNA, hairpin probe (HP), Exo III, AgNO3, and NaBH4. The functionalized HP contained a cytosine-rich oligonucleotide loop (C-rich loop), which served as an effective tem...
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#1Xue Qin (NEAU: Northeast Agricultural University)H-Index: 3
#2Hao Wang (NEAU: Northeast Agricultural University)H-Index: 4
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 9 authors...
Abstract null null The genus Cronobacter is an opportunistic food-borne pathogen which is able to adapt to diverse environments and shows considerable genetic diversity. Genomic analysis can be used to reveal the variation across the genus with respect to virulence, drug resistance and factors involved in horizontal gene transfer mechanisms, such as integrons, conjugative plasmids, and recombinases. In this study, whole-genome comparative analysis of 27 Cronobacter genomes (12 existing and 15 ne...
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#1Yuwei Ren (NEAU: Northeast Agricultural University)H-Index: 1
#2Pingping Gao (NEAU: Northeast Agricultural University)H-Index: 1
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
ABSTRACT Escherichia coli O157:H7 (E. coli O157:H7), one of the most widespread foodborne pathogens, can cause a series of diseases and even lead to death. In this study, a highly sensitive method was developed by combining aptamer-exonuclease III (Exo III)–assisted amplification with lateral flow assay (LFA) based on gold nanoparticles (AuNP). The compound of single-stranded (ss) DNA-anti-E. coli O157:H7 aptamer (ssDNA-aptamer) was formed by hybridization between designed target ssDNA and aptam...
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#1Tao Yang (NEAU: Northeast Agricultural University)H-Index: 3
#2Zhenghui Wang (NEAU: Northeast Agricultural University)H-Index: 2
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 10 authors...
Effective testing tools for Escherichia coli O157:H7 can prevent outbreaks of foodborne illness. In this paper, a smartphone-based colorimetric aptasensor was developed using functionalized gold nanoparticles (GNP) and multi-walled carbon nanotubes (MWCNT) for monitoring E. coli O157:H7 in milk. The maximum absorption peak of GNP bonded with aptamer (Apt) generated evident transformation from 518 to 524 nm. The excess GNP-Apt was removed by functionalized MWCNT magnetized with carbonyl iron powd...
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#1Pingping GaoH-Index: 1
#2Lihan WangH-Index: 2
Last. Yujun JiangH-Index: 8
view all 10 authors...
In this paper, a novel and ultrasensitive lateral flow assay (LFA) based on aptamer–magnetic separation, and multifold Au nanoparticles (AuNPs) was developed for visual detecting Salmonella enterica ser. Typhimurium (S. Typhimurium). The method realized magnetic enrichment and signal transduction via magnetic separation and achieved signal amplification through hybridizing AuNPs–capture probes and AuNPs–amplification probes to form multifold AuNPs. Two different thiolated single-strand DNA (ssDN...
Source
#1Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
#2Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
Last. Wei Zhang (NEAU: Northeast Agricultural University)H-Index: 6
view all 8 authors...
Abstract In this study, the invasion protein gene for Salmonella spp. and the 16S-23S rRNA internal transcribed spacer sequence for Cronobacter spp. were used as two species-specific targets simultaneously amplified using labelled primers in the multiplex loop-mediated isothermal amplification (mLAMP) reaction. Subsequently, the modified amplification products were monitored by lateral flow dipstick (LFD) through dual immunoreactions. The accumulation of gold nanoparticles produced characteristi...
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#1Shiqian Fu (NEAU: Northeast Agricultural University)H-Index: 5
#2Xue Qin (NEAU: Northeast Agricultural University)H-Index: 3
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 9 authors...
Due to the lack of specific genes for rapid detection methods of Cronobacter sakazakii in food samples, whole genome sequence analysis was performed in this investigation using the basic local alignment search tool. Forty-two DNA fragments unique to C. sakazakii were mined, then primers were designed and screened by PCR and loop-mediated isothermal amplification (LAMP). Two primer sets, CS1 and CS31, were found as specific and stable primers, with their corresponding nucleic acid targets the CSK...
Source
#1Xue Qin (NEAU: Northeast Agricultural University)H-Index: 3
#2Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 13
Last. Wei Zhang (NEAU: Northeast Agricultural University)H-Index: 6
view all 12 authors...
Acetochlor has been widely used globally for its effective weed control, but the dietary intake of associated residues by people has become a major concern nowadays. Milk is regarded as the best solvent to dissolve pesticides due to its fat-rich characteristic. In this study, we aimed to evaluate the transfer of acetochlor from feed to raw milk. Twenty lactating Australian Holstein cows were randomly chosen and divided into 1 control group and 3 treatment groups, feeding acetochlor at the dosage...
Source
#1Tao Yang (NEAU: Northeast Agricultural University)H-Index: 3
#2Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
ABSTRACT Escherichia coli O157:H7 is an extremely serious foodborne pathogen accounting for a vast number of hospitalizations. In this system, a simple, rapid, and safe compound method was developed based on carbonyl iron powder (CIP) and multiwalled carbon nanotubes (MWCNT). Then, the CIP@MWCNT-based aptasensor was constructed by strong π-stacking between nanocomposite and aptamer, single-strand DNA, causing fluorescent quenching of the dye-labeled aptamer. The restoration of dye fluorescence c...
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#1Sihan Chen (NEAU: Northeast Agricultural University)H-Index: 5
#2Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 7 authors...
Abstract A sensitive and accurate strategy was presented based on gold nanoparticles (AuNPs) for visual detecting viable Salmonella typhimurium (S. typhimurium). In this paper, dual aptamers were used to capture S. typhimurium and PCR amplification, respectively. The highly stable network structure formed by the hybridization between amplicons and AuNPs-probes protects AuNPs from self-aggregation and color change in the presence of salt. Under optimized conditions, the ratio of the absorbance in...
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