Sarah C. Bloch
Vanderbilt University Medical Center
FrizzledH&E stainInternal medicineClostridium difficile toxin BCellRegeneration (biology)EnzymeChemistryImmunologyIn vivoNucleic acidEx vivoColonizationCystic fibrosisAsymptomaticInflammatory bowel diseaseImmunoassayPathogenesisIntestinal epitheliumStem cellClostridioidesEpithelial DamageStructure functionClostridium difficileC difficileTwo step algorithmOrganoidRecombinant DNACancer researchInfectious disease (medical specialty)EpitheliumBiochemistryFecesMedicineCohortStainingBiologyMicrobiologyClostridium difficile toxin A
8Publications
3H-index
15Citations
Publications 8
Newest
#1Nicholas O. Markham (Vandy: Vanderbilt University)H-Index: 4
#2Sarah C. Bloch (VUMC: Vanderbilt University Medical Center)H-Index: 3
Last. D. Borden Lacy (Vandy: Vanderbilt University)H-Index: 34
view all 11 authors...
Clostridioides difficile is linked to nearly 225,000 antibiotic-associated diarrheal infections and almost 13,000 deaths per year in the United States. Pathogenic strains of C. difficile produce toxin A (TcdA) and toxin B (TcdB), which can directly kill cells and induce an inflammatory response in the colonic mucosa. Hirota, et al. first introduced the intrarectal instillation model of intoxication using TcdA and TcdB purified from VPI 10463 and 630 C. difficile strains. Here, we expand this tec...
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#1Jacob M. Parnell (VUMC: Vanderbilt University Medical Center)H-Index: 1
#2Irtiqa Fazili (UTHSC: University of Tennessee Health Science Center)H-Index: 1
Last. Maribeth R. NicholsonH-Index: 7
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OBJECTIVES Recent Infectious Disease Society of America guidelines recommend multistep testing algorithms to diagnose Clostridioides difficile infection (CDI), including a combination of nucleic acid amplification-based testing (NAAT) and toxin enzyme immunoassay (EIA). The use of these algorithms in children, including the ability to differentiate between C. difficile colonization and CDI, however, has not been evaluated. METHODS We prospectively enrolled asymptomatic pediatric patients with ca...
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#1Nicholas O. Markham (Vandy: Vanderbilt University)H-Index: 4
#2Sarah C. Bloch (VUMC: Vanderbilt University Medical Center)H-Index: 3
Last. D. Borden Lacy (Vandy: Vanderbilt University)H-Index: 34
view all 7 authors...
Abstract Clostridioides difficile is linked to nearly 225,000 antibiotic-associated diarrheal infections and almost 13,000 deaths per year in the United States. Pathogenic strains of C. difficile produce toxin A (TcdA) and toxin B (TcdB), which can directly kill cells and induce an inflammatory response in the colonic mucosa. Hirota, et al. first introduced the intrarectal instillation model of intoxication using TcdA and TcdB purified from VPI 10463 and 630 C. difficile strains. Here, we expand...
Source
#2Jacob M. ParnellH-Index: 1
Last. Kathryn M. EdwardsH-Index: 115
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Gastrointestinal infections often induce epithelial damage that must be repaired for optimal gut function. While intestinal stem cells are critical for this regeneration process [R. C. van der Wath, B. S. Gardiner, A. W. Burgess, D. W. Smith, PLoS One 8, e73204 (2013); S. Kozar et al., Cell Stem Cell 13, 626–633 (2013)], how they are impacted by enteric infections remains poorly defined. Here, we investigate infection-mediated damage to the colonic stem cell compartment and how this affects epit...
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#1Melissa A. Farrow (Vandy: Vanderbilt University)H-Index: 10
#2Nicole M. Chumber (Vandy: Vanderbilt University)H-Index: 1
Last. D. Borden Lacy (Vandy: Vanderbilt University)H-Index: 34
view all 9 authors...
Clostridiodes difficile is the leading cause of nosocomial diarrhea in the United States. The primary virulence factors are two homologous glucosyltransferase toxins, TcdA and TcdB, that inactivate host Rho-family GTPases. The glucosyltransferase activity has been linked to a ‘cytopathic’ disruption of the actin cytoskeleton and contributes to the disruption of tight junctions and the production of pro-inflammatory cytokines. TcdB is also a potent cytotoxin that causes epithelium necrotic damage...
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#2Jacob M. ParnellH-Index: 1
Last. Kathryn M. EdwardsH-Index: 115
view all 7 authors...
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