Francisco Miralles
Paris Descartes University
PromoterGeneEndocrinologyCellular differentiationGene expressionTranscriptomeRegulation of gene expressionFetusPlacentaPreeclampsiaTrophoblastPancreasDiseaseEndocrine systemGeneticsBioinformaticsMedicineTranscription factorBiologyCell biology
30Publications
16H-index
1,250Citations
Publications 35
Newest
#1Clara Apicella (University of Paris)H-Index: 2
#2Camino S. M. Ruano (University of Paris)H-Index: 1
Last. Francisco Miralles (University of Paris)H-Index: 16
view all 7 authors...
A bioinformatics screen for non-coding genes was performed from microarrays analyzing on the one hand trophoblast fusion in the BeWo cell model, and on the other hand, placental diseases (preeclampsia and Intra-Uterine Growth Restriction). Intersecting the deregulated genes allowed to identify two miRNA (mir193b and miR365a) and one long non coding RNA (UCA1) that are pivotal for trophoblast fusion, and deregulated in placental diseases. We show that miR-193b is a hub for the down-regulation of ...
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#1Camino S. M. Ruano (University of Paris)H-Index: 1
#1Camino S. M. Ruano (University of Paris)H-Index: 1
Last. Daniel Vaiman (University of Paris)H-Index: 11
view all 8 authors...
Two major obstetric diseases, preeclampsia (PE), a pregnancy-induced endothelial dysfunction leading to hypertension and proteinuria, and intra-uterine growth-restriction (IUGR), a failure of the fetus to acquire its normal growth, are generally triggered by placental dysfunction. Many studies have evaluated gene expression deregulations in these diseases, but none has tackled systematically the role of alternative splicing. In the present study, we show that alternative splicing is an essential...
1 CitationsSource
#1Aurélien Ducat (Paris V: Paris Descartes University)H-Index: 5
#2Betty Couderc (Paris V: Paris Descartes University)H-Index: 2
Last. Daniel Vaiman (Paris V: Paris Descartes University)H-Index: 45
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Summary STOX1 is a transcription factor involved in preeclampsia and Alzheimer disease. We show that the knock-down of the gene induces rather mild effect on gene expression in trophoblast cell lines (BeWo). We identified binding sites of STOX1 shared by the two major isoforms, STOX1A and STOX1B. Profiling gene expression of cells overexpressing either STOX1A or STOX1B, we identified genes downregulated by both isoforms, with a STOX1 binding site in their promoters. Among those, STOX1-induced An...
3 CitationsSource
#1Francisco Miralles (Paris V: Paris Descartes University)H-Index: 16
#2Hélène Collinot (Paris V: Paris Descartes University)H-Index: 2
Last. Daniel Vaiman (Paris V: Paris Descartes University)H-Index: 45
view all 13 authors...
Adverse long-term cardiovascular (CV) consequences of PE are well established in women. However, the mechanism responsible for that risk remains unknown. Here, we mated wild-type female mice of the FVB/N strain to STOX1A-overexpressing mice to mimic severe PE and investigated the long-term consequences on the maternal cardiovascular system. Ultrasonography parameters were analyzed in mice before pregnancy and at 3 and 6 months post-pregnancy. At 6 months post-pregnancy, cardiac stress test induc...
8 CitationsSource
#1Clara Apicella (Paris V: Paris Descartes University)H-Index: 2
#2Camino S. M. Ruano (Paris V: Paris Descartes University)H-Index: 1
Last. Daniel Vaiman (Paris V: Paris Descartes University)H-Index: 45
view all 5 authors...
In this review, we comprehensively present the function of epigenetic regulations in normal placental development as well as in a prominent disease of placental origin, preeclampsia (PE). We describe current progress concerning the impact of DNA methylation, non-coding RNA (with a special emphasis on long non-coding RNA (lncRNA) and microRNA (miRNA)) and more marginally histone post-translational modifications, in the processes leading to normal and abnormal placental function. We also explore t...
31 CitationsSource
#1Aurélien Ducat (French Institute of Health and Medical Research)H-Index: 5
#2Alexandra S Vargas (French Institute of Health and Medical Research)H-Index: 4
Last. Daniel Vaiman (French Institute of Health and Medical Research)H-Index: 45
view all 10 authors...
Aspirin (acetyl-salicylic acid) is one of the most ancient drugs of the human pharmacopeia. Nonetheless, its action at low doses is not well understood at the molecular level. One of the applications of low-dose aspirin treatment is the prevention of preeclampsia (PE) in patients at risk. Foeto-placental overexpression of the STOX1A transcription factor in mice triggers PE symptoms. Transcriptomic analysis of the placentas, showed that aspirin massively down-regulates genes of the coagulation an...
5 CitationsSource
#1Louise BiquardH-Index: 2
#2Aurélien DucatH-Index: 5
Last. Daniel VaimanH-Index: 45
view all 7 authors...
Introduction Preeclampsia is a major disease of pregnancy, affecting 2–8% of pregnant women. It is characterized by arterial hypertension and proteinuria occurring from the 20th week of amenorrhea. The STOX1 overexpressing mice develop a preeclamptic syndrome. This model is very relevant to identify potential new actors in preeclampsia because the phenotype is both precocious and severe. Objectives Using this mouse model of severe PE, we aim to identify plasma proteins quantitatively modified du...
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#1Daniel Vaiman (French Institute of Health and Medical Research)H-Index: 45
#2Rajaa Aouache (French Institute of Health and Medical Research)H-Index: 1
Last. Francisco Miralles (French Institute of Health and Medical Research)H-Index: 16
view all 3 authors...
Preeclampsia (PE) is a characterized by de novo hypertension and proteinuria. Women affected with PE have an increased risk of chronic hypertension and premature cardiovascular disease years later. Mice bearing transgenic fetuses overexpressing the human transcription factor STOX1 (storkhead box1) develop a severe preeclamptic phenotype. The molecular analysis of long term cardiovascular risks induced by preeclampsia will be studied using our mouse model. We started with 10 eight to ten-months-o...
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#1Rajaa Aouache (French Institute of Health and Medical Research)H-Index: 1
#2Louise BiquardH-Index: 2
Last. Francisco MirallesH-Index: 16
view all 4 authors...
Preeclampsia is a persistent hypertensive gestational disease characterized by high blood pressure and proteinuria, which presents from the second trimester of pregnancy. At the cellular level, preeclampsia has largely been associated with the release of free radicals by the placenta. Placenta-borne oxidative and nitrosative stresses are even sometimes considered as the major molecular determinants of the maternal disease. In this review, we present the recent literature evaluating free radical ...
119 CitationsSource
#1Céline Méhats (French Institute of Health and Medical Research)H-Index: 22
#2Francisco Miralles (French Institute of Health and Medical Research)H-Index: 16
Last. Daniel Vaiman (French Institute of Health and Medical Research)H-Index: 45
view all 3 authors...
La preeclampsie est une maladie majeure, frequente et potentiellement severe de la grossesse, caracterisee par une hypertension gravidique et une proteinurie. Dans cette revue, nous rapportons les progres recents realises dans la comprehension de la pathologie et evoquons ses impacts a long terme sur la sante vasculaire maternelle. Nous decrivons ensuite les bases genetiques, epigenetiques et immunologiques de la preeclampsie. Nous detaillons en particulier les liens entre pathologie et stress o...
1 CitationsSource