Michael Adler
Technical University of Dortmund
AnalyteGel electrophoresisBiophysicsMolecular biologyNanotechnologyChemistryNanostructureConjugateBiotinylationCombinatorial chemistryStreptavidinImmunoassayScanning Force MicroscopyHaptenProtein dnaMoleculeChromatographyPolymerase chain reactionDNABiologySupramolecular chemistry
15Publications
10H-index
788Citations
Publications 13
Newest
#1Christine WurthH-Index: 5
#2Barthélemy DemeuleH-Index: 12
Last. Michael AdlerH-Index: 10
view all 4 authors...
The International Conference on Harmonization Q8 (R2) includes a requirement that “Critical formulation attributes and process parameters are generally identified through an assessment of the extent to which their variation can impact the quality of the drug product,” that is, the need to assess the robustness of a formulation. In this article, a quality-by-designebased definition of a “robust formulation” for a biopharmaceutical product is proposed and illustrated with a case study. A multivari...
#1Anacelia Ríos Quiroz (University of Basel)H-Index: 1
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Michael AdlerH-Index: 10
view all 9 authors...
Although light obscuration is the “gold standard” for subvisible particle measurements in biopharmaceutical products, the current technology has limitations with respect to the detection of translucent proteinaceous particles and particles of sizes smaller and around 2 μm. Here, we describe the evaluation of a modified light obscuration sensor utilizing a novel measuring mode. Whereas standard light obscuration methodology monitors the height (amplitude) of the signal, the new approach monitors ...
Source
#1Margaret Speed Ricci (Genentech)H-Index: 1
#2Michelle Frazier (Amgen)H-Index: 1
Last. Hanns-Christian MahlerH-Index: 31
view all 13 authors...
Pharmaceutical scientists in the biotechnology industry have traditionally focused on achieving acceptable shelf lives of drug products in their original, unopened product unit configuration (e.g., two years stored at 2–8 °C). However, it is now clear that stability considerations extend beyond
Source
#1Hendrik SchroederH-Index: 17
#2Michael AdlerH-Index: 10
Last. Christof M. NiemeyerH-Index: 75
view all 6 authors...
We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and o...
Source
#1Bruno PignataroH-Index: 32
#2Lifeng ChiH-Index: 72
Last. Harald FuchsH-Index: 86
view all 7 authors...
Self-assembled oligomeric nanostructures consisting of bisbiotinylated DNA fragments connected by the protein streptavidin (STV) are studied by dynamic scanning force microscopy (SFM) operating in air. A comparison of the images taken in repulsive and attractive regimes is systematically made on DNA and STV structures. Stable and reproducible SFM images are obtained in the attractive regime by using a special feedback circuit, called Q-control. On the other hand, when SFM is operating in the rep...
Source
#1Christof M. Niemeyer (University of Bremen)H-Index: 75
#2Ron Wacker (University of Bremen)H-Index: 2
Last. Michael Adler (University of Bremen)H-Index: 10
view all 3 authors...
: Analysis with nanorings: The endogeneous proteins of supramolecular DNA nanocircles, obtained in high yields from oligomeric precursors containing bisbiotinylated DNA and streptavidin, are conveniently functionalized with biotinylated hapten moieties. These modular conjugates can be used as reagents in a novel competitive immunoassay for the high-sensitivity detection of low molecular weight analytes.
Source
#1Christof M. Niemeyer (University of Bremen)H-Index: 75
#2Ron Wacker (University of Bremen)H-Index: 2
Last. Michael Adler (University of Bremen)H-Index: 10
view all 3 authors...
Source
#1Song GaoH-Index: 9
#2Lifeng ChiH-Index: 72
Last. Harald FuchsH-Index: 86
view all 7 authors...
: Biomolecular complexes, nanocircles and aggregates of DNA and streptavidin (STV) are studied by dynamic scanning force microscopy. More structural details are observed with an improved dynamic mode assisted with a special feedback circuit (Q-control), as shown in the picture. Under otherwise identical conditions, these improvements indicate that the well cited models relating enlarged lateral size to the finite geometry of the SFM tip have to be modified for soft samples susceptible to tip-sam...
Source
#1Christof M. Niemeyer (University of Bremen)H-Index: 75
#2Michael AdlerH-Index: 10
Last. Lifeng ChiH-Index: 72
view all 4 authors...
Covalent conjugates consisting of streptavidin and a 24-mer single-stranded DNA oligonucleotide have been oligomerized by cross-linking with a 5‘,5‘-bis-biotinylated 169-base-pair double-stranded DNA (dsDNA) fragment. The oligomeric conjugates formed have been analyzed by nondenaturing gel electrophoresis and scanning-force microscopy (SFM). The comparison of analogous oligomers, prepared from native STV and the bis-biotinylated dsDNA fragment, revealed that the covalent STV−oligonucleotide hybr...
Source
#1Christof M. Niemeyer (University of Bremen)H-Index: 75
#2Michael Adler (University of Bremen)H-Index: 10
Last. Lifeng ChiH-Index: 72
view all 6 authors...
Oligomeric nanoparticle networks, generated by the self-assembly of bis-biotinylated double-stranded DNA fragments and streptavidin, have been studied by scanning force microscopy (SFM). SFM imaging revealed the presence within the networks of irregular thick DNA molecules, which were often associated with distinct, Y-shaped structural elements. Closer analysis revealed that the Y structures are formed by condensation (thickening and shortening) of two DNA fragments, most likely through the supe...
Source
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.