Yoshiaki Soejima
Okayama University
Messenger RNAPhosphorylationBone morphogenetic protein 7Internal medicineDownregulation and upregulationEndocrinologyReceptorGene knockdownCLOCKChemistryFollicular phaseMineralocorticoid receptorLiver receptor homolog-1HSD3B1Adrenal cortexOvaryAldosteroneFollistatinPER2ForskolinPolycystic ovaryBone morphogenetic proteinProtein expressionText miningCholesterol side-chain cleavage enzymeTranscription factorBiologyCell biology
5Publications
1H-index
4Citations
Publications 5
Newest
#1Atsuhito Suyama (Okayama University)H-Index: 1
#2Nahoko Iwata (Okayama University)H-Index: 5
Last. Fumio Otsuka (Okayama University)H-Index: 35
view all 7 authors...
The functional role of the transcription factors NR5A1 and NR5A2 and their interaction with Clock gene and bone morphogenetic proteins (BMPs) were investigated in human granulosa KGN cells. Treatment with BMP-15 and GDF-9 suppressed forskolin (FSK)-induced steroidogenesis as shown by the mRNA expression levels of StAR and P450scc but not the mRNA expression level of P450arom. Of interest, treatment with BMP-15 and GDF-9 also suppressed FSK-induced NR5A2 mRNA expression. Treatment with BMP-15 sup...
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#1Atsuhito Suyama (Okayama University)H-Index: 1
#2Nahoko Iwata (Okayama University)H-Index: 5
Last. Fumio Otsuka (Okayama University)H-Index: 35
view all 7 authors...
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#1Yoshiaki Soejima (Okayama University)H-Index: 1
#2Nahoko Iwata (Okayama University)H-Index: 5
Last. Fumio Otsuka (Okayama University)H-Index: 35
view all 8 authors...
Functional interactions between the levels of clock gene expression and adrenal steroidogenesis were studied in human adrenocortical H295R cells. Fluctuations of Bmal1, Clock, Per2 and Cry1 mRNA levels were found in H295R cells treated with forskolin (FSK) in a serum-free condition. The changes of clock gene expression levels were diverged, with Clock mRNA level being significantly higher than Cry1 and Per2 mRNA levels after 12-h stimulation with FSK. After FSK induction, mRNA levels of StAR and...
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#1Yasuhiro Nakano (Okayama University)H-Index: 3
#2Toru Hasegawa (Okayama University)H-Index: 4
Last. Fumio Otsuka (Okayama University)H-Index: 35
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Abstract Aldosterone (Aldo) is involved in various cardiovascular diseases such as hypertension and heart failure. Aldo levels are known to be increased in patients with polycystic ovary syndrome, and expression of the mineralocorticoid receptor (MR) has also been detected in the ovary. However, the effect of Aldo on reproductive function has yet to be elucidated. Here, we examined the effects of Aldo on follicular steroidogenesis using primary culture of rat granulosa cells by focusing on the o...
1 CitationsSource
#1Satoko Nagao (Okayama University)H-Index: 3
#2Nahoko Iwata (Okayama University)H-Index: 5
Last. Fumio Otsuka (Okayama University)H-Index: 35
view all 10 authors...
: A functional link between clock gene expression and ovarian steroidogenesis was studied using human granulosa KGN cells. Similarities between changes in the mRNA and protein expression levels of Bmal1 and Clock and those of Per2 and Cry1 were found in KGN cells after treatment with forskolin. Among the interrelationships between the expression levels of clock and steroidogenic factors, Clock mRNA had a strongly positive correlation with P450arom and a negative correlation with 3βHSD. Knockdown...
4 CitationsSource