Jian Wang
China Agricultural University
FermentationHeterologous expressionAnimal scienceCellulaseProinflammatory cytokineEnzymeChemistryBasal (phylogenetics)XylanaseMannoseGalactomycesChlortetracyclineGeotrichumCorn stoverPeanut oilGalactoseMealStachyoseLinseed oilCorncobLipaseAnti-inflammatoryRapeseedSoybean oilXylobioseRaffinoseDigestive enzymeCapsaicinBioconversionHydrolaseXyloseTrypsinCellobioseSunflower oilGlycosylAspergillus oryzaePichia pastorisCapsicum extractAspergillus freseniiFood scienceChromatographyBiochemistryBiologyHydrolysis
6Publications
1H-index
2Citations
Publications 6
Newest
#1Yajing Liu (CAU: China Agricultural University)H-Index: 2
#2Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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A study was carried out to investigate the characterization of a novel Aspergillus sulphureus JCM01963 xylanase (AS-xyn10A) with a carbohydrate binding module (CBM) and its application in degrading alkali pretreated corncob, rapeseed meal and corn stover alone and in combination with a commercial cellulase. In this study, the 3D structure of AS-xyn10A, which contained a CBM at C-terminal. AS-xyn10A and its CBM-truncated variant (AS-xyn10A-dC) was codon-optimized and over-expressed in Komagaella ...
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#1Shenfei Long (CAU: China Agricultural University)H-Index: 9
#2Sujie Liu (CAU: China Agricultural University)H-Index: 4
Last. Xiangshu Piao (CAU: China Agricultural University)H-Index: 22
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Abstract The objective of this study was to investigate the effects of natural capsicum extract (NCE, containing 2% natural capsaicin, the rest is carrier) replacing chlortetracycline (CTC) on performance, digestive enzyme activities, antioxidant capacity, inflammatory cytokines, and gut health in weaned pigs. A total of 108 weaned pigs (Duroc × [Landrace × Yorkshire], initial body weight = 8.68 ± 1.34 kg; weaned on d 28) were randomly allotted into 3 treatments with 6 replicate pens per treatme...
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#1Yongzhi Yang (CAU: China Agricultural University)H-Index: 1
#2Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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: β-1, 4-glucosidases generate glucose from cellobiose and oligosaccharides, enhancing the productivity in biorefinery and the bioconversion process as well as the nutritional value in food and feed. With the high-throughput sequencing technique, a novel β-1, 4-glucosidase, named bgl T2, containing 861 amino acid residues, was found from Aspergillus fresenii. bgl T2 belongs to the glycosyl hydrolase (GH) family 3. The bgl T2 that expressed by Komagataella phaffii X33 presented the highest activi...
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#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Xu Yang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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Abstract The α-galactosidase gene (galC) was cloned from Aspergillus oryzae YZ1 and expressed in Pichia pastoris. The galC (2319 bp) containing two introns encoded a protein of 726 amino acids. The activity of the α-galactosidase (GalC) increased 1-fold after coding sequence optimization. Purified GalC exhibited a single protein band (100 kDa) in SDS-PAGE. The optimum pH and temperature of GalC were pH 4.66 and 50 °C, respectively. Like many GH36 family α-galactosidases, GalC displayed its activ...
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#1Yongzhi YangH-Index: 1
#2Jian WangH-Index: 1
Last. Cao Yunhe
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#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Yajing Liu (CAU: China Agricultural University)H-Index: 2
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
view all 5 authors...
A Galactomyces geotrichum strain with lipolytic activity was isolated and identified by the analysis of internal transcribed spacer (ITS) sequence of 18 s rDNA. Full-length lipase gene of this stain is composed of 1692 base pairs (bp) without intron, which encodes a 563-amino-acid protein. A catalytic triad (Ser217–Glu354–His463) was found by constructing the three-dimensional structure of the lipase. In shake flasks, the lipase (LIP) catalytic activity in the supernatant of the recombinant Pich...
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