Elizabeth Rose Roberts
Hull York Medical School
TransgeneChemokinePathologyChemistryExtracellular matrixSkin repairTHP1 cell lineEx vivoBiopsyMacrophage polarizationHuman skinNegative-pressure wound therapyErythropoiesisWound healingFixativeChronic woundWound edgeM2 MacrophageSkin barrierDamage responseResponse to therapySingle useMedicineStainingCell biology
Publications 3
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Francesca L. Longhorne (Hull York Medical School)
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
view all 5 authors...
Negative pressure wound therapy is a widely used treatment for chronic, nonhealing wounds. Surprisingly, few studies have systematically evaluated the cellular and molecular effects of negative pressure treatment on human skin. In addition, no study to date has directly compared recently available single-use negative pressure modalities to traditional negative pressure devices in a controlled setting. Here we developed a novel large-scale ex vivo human skin culture system to effectively evaluate...
Chronic non-healing wounds, which primarily affect the elderly and diabetic, are a significant area of clinical unmet need. Unfortunately, current chronic wound treatments are inadequate, while available pre-clinical models poorly predict the clinical efficacy of new therapies. Here we describe a high throughput, pre-clinical model to assess multiple aspects of the human skin repair response. Partial thickness wounds were created in human ex vivo skin and cultured across a healing time course. S...
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Elizabeth Rose Roberts (Hull York Medical School)H-Index: 1
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
view all 7 authors...
Abstract Macrophages are important for effective iron recycling and erythropoiesis, but also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds, and a requirement of iron for effective repair. Here, we sought to determine the influence of iron on macrophage function in the context of wound healing. Interestingly, wound macrophages extensively sequestered iron throughout healing, associated with a pro-h...
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