Yurema Teijeiro-Gonzalez
King's College London
Fluorescence anisotropyPhysicsViscosityTime-resolved spectroscopyDiffusion (business)MicroscopyFluorescence spectroscopyQuantum yieldExponential decayBiological systemMaterials scienceFluorescence recovery after photobleachingRotational diffusionRhodamine 6GMolecular physicsNuclear magnetic resonanceFluorescence-lifetime imaging microscopyMolecular dynamicsAnisotropyFluorescenceFörster resonance energy transfer
3Publications
1H-index
2Citations
Publications 3
Newest
#1Yurema Teijeiro-Gonzalez ('KCL': King's College London)H-Index: 1
#2Alessandro Crnjar ('KCL': King's College London)H-Index: 3
Last. Jakub NedbalH-Index: 9
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Abstract Forster resonance energy transfer (FRET) is a powerful tool to investigate the interaction between proteins in living cells. Fluorescence proteins, such as the green fluorescent protein (GFP) and its derivatives, are co-expressed in cells linked to proteins of interest. Time-resolved fluorescence anisotropy is a popular tool to study homo-FRET of fluorescent proteins as an indicator of dimerisation, where its signature consists of a very short component at the beginning of the anisotrop...
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#1Klaus SuhlingH-Index: 41
#2Yurema Teijeiro-Gonzalez ('KCL': King's College London)H-Index: 1
Last. Carla Molteni ('KCL': King's College London)H-Index: 22
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Imaging viscosity and its spatiotemporal patterns can provide valuable insight into the underlying physical conditions of biochemical reactions and biological processes in cells and tissues. One way to measure viscosity and diffusion is the use of fluorescence recovery after photobleaching (FRAP). We combine FRAP with FLIM and time-resolved fluorescence anisotropy imaging (tr-FAIM), by acquiring time- and polarization-resolved fluorescence images in every frame of a FRAP series. This allows us t...
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#1Yurema Teijeiro-Gonzalez ('KCL': King's College London)H-Index: 1
#2A. Le Marois (Francis Crick Institute)
Last. Klaus SuhlingH-Index: 41
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We report the simultaneous combination of three powerful techniques in uorescence microscopy: Fluorescence Lifetime Imaging (FLIM), Fluorescence Anisotropy Imaging (FAIM) and Fluorescence Recovery After Photobleaching (FRAP), also called F3 microscopy. An exhaustive calibration of the setup was carried out with several rhodamine 6G (R6G) solutions in water-glycerol and from the combination of the FAIM and FRAP data, the hydrodynamic radius of the dye was directly calculated. The F3 data was anal...
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