Pedro Henrique Gonçalves Guedes
Binding siteDNA–DNA hybridizationAntigenMolecular biologyVirologyAntibodyBiosensorgenomic DNAChemistryZika virusToxoplasmosisToxoplasma gondiiNinhydrinPeptideDifferential pulse voltammetryCandida aurisElectrochemical detectionChromatographySerologyPoint of careDNADielectric spectroscopyBinding selectivity
3Publications
2H-index
12Citations
Publications 4
Newest
Abstract null null This work reports a simple strategy for Candida auris genomic DNA (gDNA) detection, a multi-resistant fungus associated with nosocomial outbreaks in healthcare settings, presenting high mortality and morbidity rates. The platform was developed using gold electrode sensitized with specific DNA capture probe and ninhydrin as a novel DNA hybridization indicator. The genosensor was able to detect C. auris in urine sample by differential pulse voltammetry and electrochemical impeda...
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Abstract null null This work reports a new and easy-to-use methodology for construction of screen-printed electrodes (SPE) based on carbon ink and its modification with 4-aminothiophenol (4-ATP) and gold nanoparticles for detection of HIV RNA. null Morphological analysis for SPE/4-ATP/AuNps+DNA probe showed increased roughness indicating that 4-ATP monolayer works as a network for connection of functionalized gold nanoparticles with specific oligonucleotide probe to the RNA target. The biosensor...
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Abstract Toxoplasma gondii is an intracellular protozoan parasite responsible for toxoplasmosis, which affects humans and animals. Serologic detection of anti-T. gondii immunoglobulins plays a crucial role in the clinical diagnosis of toxoplasmosis. In this work, a novel electrochemical immunosensor for detecting anti-T. gondii immunoglobulins is reported, based on immobilization of an in silico predicted peptide (PepB3), obtained from membrane protein of T. gondii, on the graphite electrode mod...
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