Yunhe Cao
China Agricultural University
FermentationPolysaccharideHeterologous expressionPhytaseHistamineBiological activityFusion proteinAnimal scienceImmunoglobulin ECellulaseMutantBiotechnologyMolecular biologyFactorial experimentCrossbreedEnzymeChemistrySpecific activityImmunologyWild typeFeed conversion ratioXylanaseMannoseAnaphylaxisGalactomycesSoybean mealGeotrichumCorn stoverPeanut oilBacillus subtilisCecumBacillus amyloliquefaciensGalactoseMealStachyoseLinseed oilCorncobNeutral Detergent FiberDNA shufflingGlucanaseLipaseLachnospiraceaeMannanRapeseedMast cellProlineSoybean oilXylobioseRaffinoseUrineFeed additiveBioconversionHydrolaseProbioticXyloseWeight gainTrypsinCellobioseSunflower oilGlycosylChitinAspergillus oryzaeThermostabilityPichia pastorisEnzyme assayPenicillium janczewskiiNormal dietß mannanaseFood scienceChromatographyDegranulationPhosphorusAmino acidBarrier functionGut floraBiochemistrySequence analysisFecesG alpha subunitBiologyCodon usage biasImmune systemMicrobiologyDirected evolutionHydrolysis
12Publications
7H-index
119Citations
Publications 12
Newest
#1Yajing Liu (CAU: China Agricultural University)H-Index: 2
#2Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
view all 5 authors...
A study was carried out to investigate the characterization of a novel Aspergillus sulphureus JCM01963 xylanase (AS-xyn10A) with a carbohydrate binding module (CBM) and its application in degrading alkali pretreated corncob, rapeseed meal and corn stover alone and in combination with a commercial cellulase. In this study, the 3D structure of AS-xyn10A, which contained a CBM at C-terminal. AS-xyn10A and its CBM-truncated variant (AS-xyn10A-dC) was codon-optimized and over-expressed in Komagaella ...
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#1Chengling Bao (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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AIMS This study evaluated the effects of Bacillus amyloliquefaciens (B. amyloliquefaciens) TL106 isolated from Tibetan pigs' feces, on the growth performance, immune response, intestinal barrier function, morphology of jejunum, cecum and colon, and gut microbiota in the mice with enterohemorrhagic Escherichia coli (EHEC)-induced intestinal diseases. METHODS AND RESULTS Forty female C57BL/6J mice were randomly divided into four groups: mice fed a normal diet (Control), mice oral administration of...
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#1Yongzhi Yang (CAU: China Agricultural University)H-Index: 1
#2Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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: β-1, 4-glucosidases generate glucose from cellobiose and oligosaccharides, enhancing the productivity in biorefinery and the bioconversion process as well as the nutritional value in food and feed. With the high-throughput sequencing technique, a novel β-1, 4-glucosidase, named bgl T2, containing 861 amino acid residues, was found from Aspergillus fresenii. bgl T2 belongs to the glycosyl hydrolase (GH) family 3. The bgl T2 that expressed by Komagataella phaffii X33 presented the highest activi...
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#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Xu Yang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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Abstract The α-galactosidase gene (galC) was cloned from Aspergillus oryzae YZ1 and expressed in Pichia pastoris. The galC (2319 bp) containing two introns encoded a protein of 726 amino acids. The activity of the α-galactosidase (GalC) increased 1-fold after coding sequence optimization. Purified GalC exhibited a single protein band (100 kDa) in SDS-PAGE. The optimum pH and temperature of GalC were pH 4.66 and 50 °C, respectively. Like many GH36 family α-galactosidases, GalC displayed its activ...
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#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Yajing Liu (CAU: China Agricultural University)H-Index: 2
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
view all 5 authors...
A Galactomyces geotrichum strain with lipolytic activity was isolated and identified by the analysis of internal transcribed spacer (ITS) sequence of 18 s rDNA. Full-length lipase gene of this stain is composed of 1692 base pairs (bp) without intron, which encodes a 563-amino-acid protein. A catalytic triad (Ser217–Glu354–His463) was found by constructing the three-dimensional structure of the lipase. In shake flasks, the lipase (LIP) catalytic activity in the supernatant of the recombinant Pich...
1 CitationsSource
#1Wenhan Yang (CAU: China Agricultural University)H-Index: 4
#2Yongzhi Yang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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As a feed additive, xylanase has been widely applied in the feed of monogastric animals, which contains multiple plant polysaccharides. However, during feed manufacture, the high pelleting temperatures challenge wild-type xylanases. The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanase. According to the predicted protein structure, a series of disulphide bridges and proline substitutions were created in the xylanase by PCR, and the mutants were expres...
18 CitationsSource
#1Yuyuan Yang (CAU: China Agricultural University)H-Index: 3
#2Yuanfang Fan (CAU: China Agricultural University)H-Index: 3
Last. Yongxi Ma (CAU: China Agricultural University)H-Index: 5
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Two experiments were conducted to determine the effects of adding exogenous phytase and xylanase, individually or in combination, as well as pelleting on nutrient digestibility, available energy content of wheat and the performance of growing pigs fed wheat-based diets.In Experiment 1, forty-eight barrows with an initial body weight of 35.9±0.6 kg were randomly assigned to a 2×4 factorial experiment with the main effects being feed form (pellet vs meal) and enzyme supplementation (none, 10,000 U...
11 CitationsSource
#1Honglei Pei (CAU: China Agricultural University)H-Index: 3
#2Xiaojing Guo (CAU: China Agricultural University)H-Index: 2
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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In order to improve some characteristics of a β-1,3-1,4-glucanase from Bacillus subtilis MA139, directed evolution was conducted in this study. After error-prone PCR, the β-1,3-1,4-glucanase gene, glu-opt, was cloned into the vector pBGP1 and transformed into Pichia pastoris X-33 to construct a mutant library. Three variants named as 7-32, 7-87, and 7-115 were screened from 8000 colonies. Amino-acid sequence analysis showed that these mutants had one or two amino-acid substitutions (7-32: T113S,...
8 CitationsSource
#1J. N. Lv (CAU: China Agricultural University)H-Index: 1
#2Yong Chen (CAU: China Agricultural University)H-Index: 4
Last. Bing Dong (CAU: China Agricultural University)H-Index: 6
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A total of 288 crossbred (DurocLandraceYorkshire) growing pigs were used in two experiments to investigate the effects of adding -mannanase to corn-soybean meal-based diets on pig performance and apparent total tract digestibility (ATTD). Both experiments lasted 28 d and were split into two phases namely 1 to 14 days (phase 1) and 15 to 28 days (phase 2). In Exp. 1,144 pigs weighing 23.601.59 kg BW were assigned to one of four corn-soybean meal-based diets containing 0, 200, 400 or 600 U/kg ...
25 CitationsSource
#1Junnan Lv (CAU: China Agricultural University)H-Index: 3
#2Yiqun Chen (CAU: China Agricultural University)H-Index: 5
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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The β-mannanase gene (1,029 nucleotide) from Bacillus subtilis MAFIC-S11, encoding a polypeptide of 342 amino acids, was cloned and expressed in Pichia pastoris. To increase its expression, the β-mannanase gene was optimized for codon usage (mannS) and fused downstream to a sequence-encoding modified α-factor signal peptide. The expression level was improved by 2-fold. This recombinant enzyme (mannS) showed its highest activity of 24,600 U/mL after 144-h fermentation. The optimal temperature and...
18 CitationsSource