Changlan Chen
Liaoning University
CytotoxicityHigh-performance liquid chromatographyChemistryPhytochemicalVillosaWestern blotAntioxidantApigeninFlavonoidBioactive compoundNatural productSilica gelDichloromethanePatrinia villosaPatrinia scabiosaefoliaCell cycleChemical constituentsPerennial herbChromatographyCancer researchp38 mitogen-activated protein kinasesColumn chromatographyFlow cytometryTraditional medicineCell cultureBiology
Publications 4
#1Liu Yangcheng (Liaoning University)H-Index: 1
#2Nan Sui (LNUTCM: Liaoning University of Traditional Chinese Medicine)
Last. Changlan Chen (Liaoning University)H-Index: 2
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Patrinia villosa (Thunb.) Juss (P. villosa), a perennial herb, is widely used as a medicinal plant in Chinese folk. This study aims to isolate and identify the chemical constituents from P. villosa and evaluate their antioxidant activity. Normal silica column chromatography, ODS silica column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC methods were used to obtain a new compound named 3-n-pentadecyl-4'-methoxyluteolin (1) and two known compounds including luteol...
#1Liu Yangcheng (Liaoning University)H-Index: 1
#2Wei Liu (Liaoning University)H-Index: 2
Last. Zheng Xiang (Liaoning University)H-Index: 2
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1 CitationsSource
#1Yue Li (Capital Medical University)H-Index: 1
#2Xiaoyan ChengH-Index: 1
Last. Qi Wang (Capital Medical University)H-Index: 1
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Abstract Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Apigenin was widely used in HCC treatment; however, the detailed mechanisms have not been clarified. We isolated, characterized, and identified Apigenin from the P. villosa plant using ethanol-extracted, semi-preparative HPLC and NMR. MTT was used to detect the cytotoxicity of Apigenin in HepG2, SMMC-7721 and Huh-7 cell lines. The cell cycle changes of Apigenin on HepG2 using flow cytometry and the key mole...
15 CitationsSource
#1Liu Yangcheng (Liaoning University)H-Index: 1
#2Wei Liu (Liaoning University)H-Index: 2
Last. Liu Hongwei (PRC: China Medical University (PRC))H-Index: 1
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BACKGROUND AND PURPOSE: Patrinia villosa Juss is an important Chinese herbal medicine widely used for thousands of years, but few reports on the ingredients of the herb have been presented. In this study, we aim to isolate the bioactive compound from the plant. MATERIAL AND METHODS: The air-dried leaves of P. villosa (15kg) were extracted three times with 70% EtOH under reflux. The condensed extract was suspended in H2O and partitioned with light petroleum, dichloromethane and n-BuOH. The dichlo...
4 CitationsSource