Isabelle I. Salles-Crawley
Imperial College London
Internal medicinePhenotypeNeutrophil extracellular trapsInnate immune systemGenetically modified mouseMolecular biologyReceptorChemistryIntegrinThrombomodulinImmunologyThrombinHemostasisVon Willebrand factorVenous thrombosisGPVIBAMBIProtein CThrombusExtracellularPopulationHemostatic functionPlatelet activationMechanosensitive channelsMedicineIntracellularBiologyPlateletFilopodiaCell biologyPharmacology
17Publications
6H-index
65Citations
Publications 14
Newest
#1Yu Huang (Imperial College London)H-Index: 12
#2Boram Gu (Imperial College London)H-Index: 8
Last. Rongjun Chen (Imperial College London)H-Index: 20
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Clinical use of tissue plasminogen activator (tPA) in thrombolytic therapy is limited by its short circulation time and hemorrhagic side effects. Inspired by fibrinogen binding to activated platelets, we report a fibrinogen-mimicking, multiarm nanovesicle for thrombus-specific tPA delivery and targeted thrombolysis. This biomimetic system is based on the lipid nanovesicle coated with polyethylene glycol (PEG) terminally conjugated with a cyclic RGD (cRGD) peptide. Our experiments with human bloo...
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#1Adela Constantinescu-Bercu (Imperial College London)H-Index: 2
#2Isabelle I. Salles-Crawley (Imperial College London)H-Index: 6
Last. James T. B. Crawley (Imperial College London)H-Index: 30
view all 3 authors...
3 CitationsSource
#1Adela Constantinescu-Bercu (Imperial College London)H-Index: 2
#2Luigi Grassi (University of Cambridge)H-Index: 60
Last. James T. B. Crawley (Imperial College London)H-Index: 30
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Platelet-neutrophil interactions are important for innate immunity, but also contribute to the pathogenesis of deep vein thrombosis, myocardial infarction and stroke. Here we report that, under flow, von Willebrand factor/glycoprotein Iba-dependent platelet 'priming' induces integrin aIIbb3 activation that, in turn, mediates neutrophil and T-cell binding. Binding of platelet aIIbb3 to SLC44A2 on neutrophils leads to mechanosensitive-dependent production of highly prothrombotic neutrophil extrace...
22 CitationsSource
#1Adela Constantinescu-Bercu (Imperial College London)H-Index: 2
#2Luigi Grassi (University of Cambridge)H-Index: 60
Last. James T. B. Crawley (Imperial College London)H-Index: 30
view all 6 authors...
Source
#1Claire Peghaire (NIH: National Institutes of Health)H-Index: 9
#2Neil Dufton (NIH: National Institutes of Health)H-Index: 13
Last. Anna M. Randi (NIH: National Institutes of Health)H-Index: 30
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Endothelial cells actively maintain an anti-thrombotic environment; loss of this protective function may lead to thrombosis and systemic coagulopathy. The transcription factor ERG is essential to maintain endothelial homeostasis. Here, we show that inducible endothelial ERG deletion (ErgiEC-KO) in mice is associated with spontaneous thrombosis, hemorrhages and systemic coagulopathy. We find that ERG drives transcription of the anticoagulant thrombomodulin (TM), as shown by reporter assays and ch...
9 CitationsSource
#1Magdalena Gierula (Imperial College London)H-Index: 8
#2Isabelle I. Salles-Crawley (Imperial College London)H-Index: 6
Last. Josefin Ahnström (Imperial College London)H-Index: 16
view all 7 authors...
BACKGROUND: Activated protein C (APC)-mediated inactivation of factor (F)Va is greatly enhanced by protein S. For inactivation to occur, a trimolecular complex among FVa, APC, and protein S must form on the phospholipid membrane. However, direct demonstration of complex formation has proven elusive. OBJECTIVES: To elucidate the nature of the phospholipid-dependent interactions among APC, protein S, and FVa. METHODS: We evaluated binding of active site blocked APC to phospholipid-coated magnetic ...
5 CitationsSource
#1James T. B. Crawley (Hammersmith Hospital)H-Index: 30
#1James T. B. Crawley (Hammersmith Hospital)
Last. Isabelle I. Salles‐Crawley (Hammersmith Hospital)
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BACKGROUND: Bone morphogenetic and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein related to the type I transforming growth factor- β (TGF-β) receptor family that is present on both platelets and endothelial cells (ECs). Bambi-deficient mice exhibit reduced hemostatic function and thrombus stability characterized by an increased embolization. OBJECTIVE: We aimed to delineate how BAMBI influences endothelial function and thrombus stability. METHODS: Bambi-deficient mice were ...
2 CitationsSource
#1Adrienn Teraz‐Orosz (Imperial College London)H-Index: 3
#2Nichola Cooper (Imperial College London)H-Index: 19
Last. Isabelle I. Salles-Crawley (Imperial College London)H-Index: 6
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5 CitationsSource
#1Kieron South (Imperial College London)H-Index: 10
#2Frederik Denorme (Katholieke Universiteit Leuven)H-Index: 14
Last. David A. Lane (Imperial College London)H-Index: 73
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: Essentials ADAMTS13 requires a substrate-induced conformational change to attain full activity in vitro. The efficacy of wild type ADAMTS13 in models of thrombosis/stroke may be enhanced by pre-activation. A pre-activated ADAMTS13 variant exhibits enhanced proteolysis of platelet agglutinates. This ADAMTS13 variant is protective in a murine model of stroke at a lower dose than WT ADAMTS13. SUMMARY: Background ADAMTS-13 circulates in a closed conformation, only achieving full proteolytic activi...
7 CitationsSource