Stefano Percio
Polytechnic University of Milan
GeneGene silencingDownregulation and upregulationCell migrationmicroRNAGene expressionApoptosisHMGA2SurvivinAcute promyelocytic leukemiaLeukemiaMyeloid leukemiaBone marrowRetinoic acidDoxorubicinAlu elementCancer researchLong non-coding RNARNAMedicineBiology
10Publications
5H-index
78Citations
Publications 10
Newest
#1Silvia MartiniH-Index: 3
#2Valentina ZucoH-Index: 25
Last. Nadia ZaffaroniH-Index: 66
view all 10 authors...
Triple-negative breast cancer (TNBC) is an aggressive disease with limited therapeutic options. Here, we pursued a combinatorial therapeutic approach to enhance the activity of selinexor, the first-in-class XPO1 inhibitor, by miR-34a ectopic expression in human TNBC experimental models. Anti-proliferative activity induced by selinexor and miR-34a expression, singly and in combination, was evaluated by MTS assay and cell counting. The effect of treatments on survivin and apoptosis-related protein...
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Background Dedifferentiated liposarcoma (DDLPS), a tumor that lacks effective treatment strategies and is associated with poor outcomes, expresses amplified MDM2 in the presence of wild-type p53. MDM2 ubiquitination of p53 facilitates its XPO1-mediated nuclear export, thus limiting p53 tumor suppressor functions. Consequently, nuclear export is a rational target in DDLPS. We directly compared the antitumor activity of the first-in class XPO1 inhibitor selinexor and doxorubicin, the standard fron...
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#1Rihan El BezawyH-Index: 3
#2Martina Tripari (University of Liverpool)H-Index: 1
Last. Nadia ZaffaroniH-Index: 66
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Speckle-type POZ (pox virus and zinc finger protein) protein (SPOP) is the most commonly mutated gene in prostate cancer (PCa). Recent evidence reports a role of SPOP in DNA damage response (DDR), indicating a possible impact of SPOP deregulation on PCa radiosensitivity. This study aimed to define the role of SPOP deregulation (by gene mutation or knockdown) as a radiosensitizing factor in PCa preclinical models. To express WT or mutant (Y87N, K129E and F133V) SPOP, DU145 and PC-3 cells were tra...
1 CitationsSource
#1Federica RotundoH-Index: 2
#2Denis CominettiH-Index: 14
Last. Paolo GandelliniH-Index: 25
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The development of novel therapies or the improvement of currently used approaches to treat prostate cancer (PCa), the most frequently diagnosed male tumor in developed countries, is an urgent need. In this regard, the functional characterization of microRNAs, molecules shown to regulate a number of cancer-related pathways, is instrumental to their possible clinical exploitation. Here, we demonstrate the tumor-suppressive role of the so far uncharacterized miR-1272, which we found to be signific...
1 CitationsSource
#1Stefano PercioH-Index: 5
#2Federica RotundoH-Index: 2
Last. Paolo Gandellini (University of Milan)H-Index: 25
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Abstract Although the role of miR-205 has been widely elucidated, the function of its host gene (MIR205HG) is yet to be clarified. We have recently investigated whether this gene is a simple endorsement for miRNA production or it may act independently, demonstrating its action as nuclear long noncoding RNA able to control basal-luminal differentiation in the human prostate context, thus deserving the reannotation as LEADR, Long Epithelial Alu-interacting Differentiation-related RNA. Here, we des...
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#1Silvia StacchiottiH-Index: 61
#2Valentina ZucoH-Index: 25
Last. Nadia ZaffaroniH-Index: 66
view all 21 authors...
Epithelioid sarcoma (ES) is a rare mesenchymal malignancy marked by SMARCB1/INI1 deficiency. Retrospective clinical data report on the activity of anthracycline- and gemcitabine-based regimens. EZH2 inhibitors are currently being tested in clinical trials. Since comparisons of these agents are unlikely to be prospectively evaluated in the clinics, we took advantage of an INI1-deficient proximal-type ES patient-derived xenograft (PDX ES-1) to comparatively assess its preclinical antitumor activit...
6 CitationsSource
#1Valentina ProfumoH-Index: 7
#2Barbara ForteH-Index: 2
Last. Paolo GandelliniH-Index: 25
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Though miR-205 function has been largely characterized, the nature of its host gene, MIR205HG, is still completely unknown. Here, we show that only lowly expressed alternatively spliced MIR205HG transcripts act as de facto pri-miRNAs, through a process that involves Drosha to prevent unfavorable splicing and directly mediate miR-205 excision. Notably, MIR205HG-specific processed transcripts revealed to be functional per se as nuclear long noncoding RNA capable of regulating differentiation of hu...
21 CitationsSource
#1Jessica Migliavacca (UniSR: Vita-Salute San Raffaele University)H-Index: 1
Last. Nadia ColtellaH-Index: 15
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// Jessica Migliavacca 1, 2 , Stefano Percio 3 , Roberta Valsecchi 1, 4 , Elisabetta Ferrero 1 , Antonello Spinelli 5 , Maurilio Ponzoni 2, 6 , Cristina Tresoldi 7 , Linda Pattini 3 , Rosa Bernardi 1, * , Nadia Coltella 1, * 1 Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy 2 Vita-Salute San Raffaele University School of Medicine, Milan, Italy 3 Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milan, Italy 4 Department of ...
8 CitationsSource
#1Stefano Percio (Polytechnic University of Milan)H-Index: 5
#2Nadia Coltella (UniSR: Vita-Salute San Raffaele University)H-Index: 15
Last. Linda Pattini (Polytechnic University of Milan)H-Index: 17
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Background Acute promyelocytic leukemia (APL) is a sub-type of acute myeloid leukemia (AML) characterized by a block of myeloid differentiation at the promyelocytic stage and the predominant t(15:17) chromosomal translocation. We have previously determined that cells from APL patients show increased expression of genes regulated by hypoxia-inducible transcription factors (HIFs) compared to normal promyelocytes. HIFs regulate crucial aspects of solid tumor progression and are currently being impl...
12 CitationsSource
#1Nadia ColtellaH-Index: 15
#2Stefano Percio (Polytechnic University of Milan)H-Index: 5
Last. Rosa BernardiH-Index: 28
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Acute promyelocytic leukemia (APL) is epitomized by the chromosomal translocation t(15;17) and the resulting oncogenic fusion protein PML-RARa. Although acting primarily as a transcriptional repressor, PML-RARa can also exert functions of transcriptional co-activation. Here, we find that PML-RARa stimulates transcription driven by HIF factors, which are critical regulators of adaptive responses to hypoxia and stem cell maintenance. Consistently, HIF-related gene signatures are upregulated in leu...
25 CitationsSource