Xiaojian Ouyang
Guangzhou University of Chinese Medicine
AdductElectron transferRadicalMedicinal chemistryMass spectrometryInhibitory postsynaptic potentialAqueous solutionChemistryStructure–activity relationshipAcid dissociation constantBromidePyrogallolGalactosideLicochalcone AGalanginAntioxidantMyricetinScutellareinSubstituentFerroptosisAstringinAcacetinFlavonolsAglyconeDimerIsorhapontigeninPiceatannolGlucuronidationScutellarinRhapontigeninABTSDPPHResonance effectTandem mass spectrometryMass spectrumElectrospray ionizationChromatographyRedoxStereochemistry
7Publications
5H-index
61Citations
Publications 7
Newest
#1Ban ChenH-Index: 8
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Dongfeng ChenH-Index: 22
view all 6 authors...
Synthetic arylamines and dietary phytophenolics could inhibit ferroptosis, a recently discovered regulated cell death process. However, no study indicates whether their inhibitory mechanisms are inherently different. Herein, the ferroptosis-inhibitory mechanisms of selected ferrostatin-1 (Fer-1) and two dietary stilbenes (piceatannol and astringin) were compared. Cellular assays suggested that the ferroptosis-inhibitory and electron-transfer potential levels decreased as follows: Fer-1 >> piceat...
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#1Xiaojian Ouyang (Guangzhou University of Chinese Medicine)H-Index: 5
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 10 authors...
Erastin-treated bone marrow-derived mesenchymal stem cells (bmMSCs) were prepared and used to compare the ferroptosis inhibitory bioactivities of four monostilbenes, including rhapontigenin (1a), isorhapontigenin (1b), piceatannol-3′-O-glucoside (1c), and rhapontin (1d). Their relative levels were 1c ≈ 1b > 1a ≈ 1d in 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and flow ...
3 CitationsSource
#1Xican LiH-Index: 19
#2Xiaojian OuyangH-Index: 5
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
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The biological process, 3-O-galactosylation, is important in plant cells. To understand the mechanism of the reduction of flavonol antioxidative activity by 3-O-galactosylation, myricetin-3-O-galactoside (M3OGa) and myricetin aglycone were each incubated with 2 mol α,α-diphenyl-β-picrylhydrazyl radical (DPPH•) and subsequently comparatively analyzed for radical adduct formation (RAF) products using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-fl...
9 CitationsSource
#1Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
#2Xiaojian Ouyang (Guangzhou University of Chinese Medicine)H-Index: 5
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 4 authors...
To probe the effect of 3′,8″-dimerization on antioxidant flavonoids, acacetin and its 3′,8″-dimer isoginkgetin were comparatively analyzed using three antioxidant assays, namely, the ·O2− scavenging assay, the Cu2+ reducing assay, and the 2,2′-azino bis(3-ethylbenzothiazolin-6-sulfonic acid) radical scavenging assay. In these assays, acacetin had consistently higher IC50 values than isoginkgetin. Subsequently, the acacetin was incubated with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxy radicals...
14 CitationsSource
#1Minshi Liang (Guangzhou University of Chinese Medicine)H-Index: 4
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
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Echinatin and its 1,1-dimethyl-2-propenyl derivative licochalcone A are two chalcones found in the Chinese herbal medicine Gancao. First, their antioxidant mechanisms were investigated using four sets of colorimetric measurements in this study. Three sets were performed in aqueous solution, namely Cu2+-reduction, Fe3+-reduction, and 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-scavenging measurements, while 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•)-scavenging colori...
21 CitationsSource
#1Qianru Liu (Guangzhou University of Chinese Medicine)H-Index: 3
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 4 authors...
To explore whether and how glucuronidation affects pyrogallol-type phytophenols, scutellarein and scutellarin (scutellarein-7-O-glucuronide) were comparatively investigated using a set of antioxidant analyses, including spectrophotometric analysis, UV-vis spectra analysis, and ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis. In spectrophotometric analyses of the scavenging of 1,1-diphe...
13 CitationsSource
#1Xiaojian Ouyang (Guangzhou University of Chinese Medicine)H-Index: 5
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 5 authors...
To clarify the role of the B-ring in antioxidant flavonols, we performed a comparative study between galangin with a null B-ring and 3,5,7-trihydroxychromone without a B-ring using five spectrophotometric assays, namely, •O2−-scavenging, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•)-scavenging, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging, 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging, and Fe3+-reducing activity. The DPPH•-scavenging reac...
10 CitationsSource