Karolina Wanat
Medical University of Łódź
PharmacokineticsXenobioticBiological activityBiological membraneCoefficient of determinationOrganismQuantitative structure–activity relationshipHigh-performance liquid chromatographyBiochemical engineeringChemistryThin-layer chromatographyIn silicoBlood–brain barrierHuman serum albuminDrugBlood brain barrier permeabilityComputer scienceChromatographyActive ingredientPlasma protein bindingBiochemistryCell biologyDistribution (pharmacology)
5Publications
3H-index
18Citations
Publications 5
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#1Karolina Wanat (Medical University of Łódź)H-Index: 3
#2Grażyna Żydek (Medical University of Łódź)H-Index: 4
Last. Elżbieta Brzezińska (Medical University of Łódź)H-Index: 9
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Plasma protein binding is an important determinant of the pharmacokinetic properties of chemical compounds in living organisms. The aim of the present study was to determine the index of protein binding affinity based on chromatographic experiments. The question is which chromatographic environment will best mimic the drug-protein binding conditions. Retention data from normal phase thin-layer liquid chromatography (NP TLC), reversed phase (RP) TLC and HPLC chromatography experiments with 129 ac...
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#1Karolina WanatH-Index: 3
#2Grażyna ŻydekH-Index: 4
Last. Elżbieta BrzezińskaH-Index: 9
view all 3 authors...
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#1Karolina Wanat (Medical University of Łódź)H-Index: 3
Drug-protein binding plays a key role in determining the pharmacokinetics of a drug. The distribution and protein binding ability of a drug changes over a lifetime, and are important considerations during pregnancy and lactation. Although proteins are a significant fraction in plasma composition, they also exist beyond the bloodstream and bind with drugs in the skin, tissues or organs. Protein binding influences the bioavailability and distribution of active compounds, and is a limiting factor i...
3 CitationsSource
#1Anna W. Sobańska (Medical University of Łódź)H-Index: 10
#2Karolina Wanat (Medical University of Łódź)H-Index: 3
Last. Elżbieta Brzezińska (Medical University of Łódź)H-Index: 9
view all 3 authors...
8 CitationsSource
#1Karolina Wanat (Medical University of Łódź)H-Index: 3
#2Elżbieta Brzezińska (Medical University of Łódź)H-Index: 9
Last. Anna W. Sobańska (Medical University of Łódź)H-Index: 10
view all 3 authors...
: In recent decades, drug-protein interactions have been widely studied and several methods of analysis of these phenomena have been developed and improved. These can be classified into separation, physical, chromatographic and electrophoretic methods. This review depicts the assumptions and mechanisms of methods from each group, details their strengths and weaknesses, and presents examples of their usage from the literature. Equilibrium dialysis, ultrafiltration, Hummel-Dreyer method or high pe...
5 CitationsSource