Radka Koblovská
Institute of Chemical Technology in Prague
High-performance liquid chromatographyChemistrySupercritical fluid extractionDaidzinIsoflavonoidApigeninBiochanin AIsoflavonesRutaOnoninGlyciteinBryopsidaDaidzeinRutaceaeChromatographyReversed-phase chromatographyBotanyLiquid chromatography–mass spectrometryTaxonBiology
9Publications
5H-index
129Citations
Publications 9
Newest
The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I 5 0 values of the standard curves range between 0.3-1.2 ng/ml. The cross reactivities to oth...
2 CitationsSource
#1Khaled Abdulmanea (Institute of Chemical Technology in Prague)H-Index: 1
#2Elena A. Prokudina (Institute of Chemical Technology in Prague)H-Index: 2
Last. Oldřich Lapčík (Institute of Chemical Technology in Prague)H-Index: 14
view all 7 authors...
Ethanolic extracts of 23 species of the Apiaceae family were analyzed for their flavonoid and isoflavonoid content, using the following methods: high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS); semi-preparative HPLC with Enzyme Linked Immunosorbent Assay (HPLC-ELISA); and immunoaffinity extraction (IAE). Rutin and quercetin-3-glucoside were the most abundant flavonoid glycosides and the most abundant flavonoid aglycone was apigenin. The isoflavonoids daidzein, gen...
13 CitationsSource
#1Radka KoblovskáH-Index: 5
#2Zuzana MackovaH-Index: 4
Last. Oldřich LapčíkH-Index: 14
view all 6 authors...
Enzyme-linked immunosorbent assays in combination with semi-preparative high-performance liquid chromatography (HPLC) and analytical HPLC with mass spectroscopy in the selective ion monitoring mode were used for the determination of selected isoflavones, daidzein, genistein, biochanin A and their homologues, in 20 representatives of the Rutaceae family. Species belonging to five genera were studied, namely Citrus, Fortunella, Poncirus, Ruta and Severinia. The enzyme immunoassays used were based ...
12 CitationsSource
#1Radka KoblovskáH-Index: 5
#1R. KoblovskáH-Index: 2
Last. Oldřich LapčíkH-Index: 14
view all 4 authors...
2 CitationsSource
#1Radka KoblovskáH-Index: 5
#1R. KoblovskáH-Index: 2
Last. Oldřich LapčíkH-Index: 14
view all 4 authors...
1 CitationsSource
#1Zuzana Mackova (Institute of Chemical Technology in Prague)H-Index: 4
#2Radka Koblovská (Institute of Chemical Technology in Prague)H-Index: 5
Last. Oldrich Lapcik (Institute of Chemical Technology in Prague)H-Index: 5
view all 3 authors...
Abstract Common emphasis of the fact that isoflavonoids are characteristic metabolites of leguminous plants sometimes leads to overlooking that the presence of isoflavonoids has been reported in several dozen other families. The spectrum of isoflavonoid producing taxa includes the representatives of four classes of multicellular plants, namely the Bryopsida, the Pinopsida, the Magnoliopsida and the Liliopsida. A review, recently published by Reynaud et al. [Reynaud, J., Guilet D., Terreux R., Lu...
57 CitationsSource
#1O. LapčíkH-Index: 1
#1Oldřich LapčíkH-Index: 14
Last. Bořivoj Klejdus (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 35
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Extracts from Arabidopsis thaliana leaves and inflorescence stalks and from Lepidium sativa (Brassicaceae) seedlings were analysed by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples, in order to determine presence of isoflavonoids. Individual ELISAs were specific for daidzein, genistein, biochanin A and for their derivatives substituted either at the 4′- or at the 7- positions. Both analytical approaches revealed homologous spectra of isoflavon...
18 CitationsSource
#1Zuzana MackovaH-Index: 4
#2Radka KoblovskáH-Index: 5
Last. Oldrich LapcikH-Index: 5
view all 3 authors...
Common emphasis of the fact that isoflavonoids are characteristic metabolites of leguminous plants sometimes leads to overlooking that the presence of isoflavonoids has been reported in several dozen other families. The spectrum of isoflavonoid producing taxa includes the representatives of four classes of multicellular plants, namely the Bryopsida, the Pinopsida, the Magnoliopsida and the Liliopsida. A review, recently published by Reynaud et al. [Reynaud, J., Guilet D., Terreux R., Lussignol M...
#1Bořivoj Klejdus (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 35
#2Lea Lojková (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 11
Last. Vlastimil KubáňH-Index: 26
view all 6 authors...
An efficient method of modifier addition for supercritical fluid extraction (SFE) of polar isoflavones was developed and yielded extraordinarily high recoveries. To find the optimal extraction conditions, a temperature and pressure optimization and modifier impact study was performed in naturally contaminated and spiked samples. Ultra-fast high-performance liquid chromatography/mass spectrometry (HPLC/MS) was used for the determination of isoflavones on an AtlantisTM dC18 high-speed reversed pha...
65 CitationsSource
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