Joo Hwan Kim
Ministry of Food and Drug Safety
CarcinogenOptometryDNA damageMolecular biologyArtificial intelligencePattern recognitionChemistryTUNEL assayIn vitroPhototoxicityIn vivoSinglet oxygenSperm motilityDimethyl sulfoxideTest (assessment)GenotoxicityComet assayMTT assaySuperoxideSodium phosphate bufferPharmaceutical SubstancesIn vitro testEye irritationUltraviolet aTiered approachComputer scienceChromatographyReactive oxygen speciesPotencyMedicinePhotochemistryCell cultureBiologyCometReproductive toxicity
10Publications
3H-index
39Citations
Publications 5
Newest
#1Hye Lyun JeonH-Index: 2
#2Ah Rang ChoH-Index: 1
Last. Ki Sook ParkH-Index: 2
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#1Kyung Yuk Ko (Ministry of Food and Drug Safety)H-Index: 4
#2Hye Lyun Jeon (Ministry of Food and Drug Safety)H-Index: 2
Last. Ki Sook Park (Ministry of Food and Drug Safety)H-Index: 2
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Abstract In order to overcome the limitations of single in vitro eye irritation tests, Integrated Approaches to Testing Assessment strategies have been suggested for evaluating eye irritation. This study developed two tiered approaches combining alternative test methods. They were designed in consideration of the solubility property of test chemicals and to use the RhCE tests at final steps. The tiered approach A is composed of the STE, BCOP, HET-CAM or RhCE tests, whereas the tiered approach B ...
2 CitationsSource
#1Yoon-hee Hong (Ministry of Food and Drug Safety)H-Index: 2
#2Hye Lyun Jeon (Ministry of Food and Drug Safety)H-Index: 2
Last. Jong Kwon Lee (Ministry of Food and Drug Safety)H-Index: 23
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Abstract Evaluation of DNA damage is critical during the development of new drugs because it is closely associated with genotoxicity and carcinogenicity. The in vivo comet assay to assess DNA damage is globally harmonized as OECD TG 489. However, a comet test guideline that evaluates DNA damage without sacrificing animals does not yet exist. The goal of this study was to select an appropriate cell line for optimization of the in vitro comet assay to assess DNA damage. We then evaluated the predi...
10 CitationsSource
#1Hye Lyun Jeon (Ministry of Food and Drug Safety)H-Index: 2
#2Jung-Sun Yi (Ministry of Food and Drug Safety)H-Index: 4
Last. Soo Jung Sohn (Ministry of Food and Drug Safety)H-Index: 4
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Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving ...
8 CitationsSource
#1Yong Sun Lee (Ministry of Food and Drug Safety)H-Index: 2
#2Jung-Sun Yi (Ministry of Food and Drug Safety)H-Index: 4
Last. Jong Kwon Lee (Ministry of Food and Drug Safety)H-Index: 23
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With ultraviolet and visible light exposure, some pharmaceutical substances applied systemically or topically may cause phototoxic skin irritation. The major factor in phototoxicity is the generation of reactive oxygen species (ROS) such as singlet oxygen and superoxide anion that cause oxidative damage to DNA, lipids and proteins. Thus, measuring the generation of ROS can predict the phototoxic potential of a given substance indirectly. For this reason, a standard ROS assay (ROS assay) was deve...
13 CitationsSource