He-Ping Zeng
South China University of Technology
Hydrogen bondCrystal structureBiological activityInternal medicineAutocrine signallingSuzuki reactionEndocrinologyChemistryApoptosisMesenchymal stem cellIn vitroCholesterolStearic acid8-HydroxyquinolineEthyl acetateMTT assayBone morphogenetic protein 4Stem cellPlastrum testudinisBone morphogenetic proteinProgrammed cell deathTransfectionFlow cytometryBiochemistryCell growthSignal transductionStereochemistryCell cultureBiologyCell biologyPharmacology
20Publications
8H-index
246Citations
Publications 15
Newest
#1Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
#1Xican Li (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 10 authors...
: Molecules that enhance chondrogenic differentiation in mesenchymal stem cells (MSCs) were identified and isolated using an in vitro Gli reporter gene assay in MSCs incorporating a Sonic Hedgehog (Shh) target. Atractylenolide III, which promoted Gli1-mediated transcriptional activity, was isolated from an ethyl acetate extract of the Rhizoma, Atractylodis macrocephalae. After dehydration, atractylenolide III was transformed to atractylenolide I. Both atractylenolides were confirmed by MS, UV, I...
30 CitationsSource
#1Tingting Wang (SCUT: South China University of Technology)H-Index: 13
#1Ting-Ting Wang (SCUT: South China University of Technology)H-Index: 1
Last. Dong-Feng Chen (GU: Guangzhou University)H-Index: 6
view all 4 authors...
Plastrum Testudinis (PT) is often used as an important traditional Chinese medicine to treat bone diseases in China for centuries. To identify active components of PT involved in promoting proliferation of MSCs, PT was extracted with ethyl acetate and separated by silica gel column chromatography with gradient elution. Sixteen (Ts-1 ∼ 16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells (...
12 CitationsSource
#1Dongfeng Chen (NU: Nanjing University)H-Index: 22
#1Dong-Feng Chen (GU: Guangzhou University)H-Index: 4
Last. Zi-Chun Hua (NU: Nanjing University)H-Index: 45
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Identifying small molecules that suppress apoptosis is promising for the therapy of brain diseases. We recently showed that autocrine bone morphogenetic protein (BMP) signaling involves the effects of cholesterol myristate present in traditional Chinese medicine on mesenchymal stem cells. The present study evaluated the effects of cholesterol myristate on the apoptosis and BMP signaling of PC12 cells. PC12 cells transfected by the inhibitor of differentiation (Id1) promoter reporter construct ta...
4 CitationsSource
#1Dongfeng Chen (NU: Nanjing University)H-Index: 22
#1Dong-Feng Chen (GU: Guangzhou University)H-Index: 4
Last. Zi-Chun Hua (NU: Nanjing University)H-Index: 4
view all 8 authors...
To identify small molecules that suppress the apoptosis of mesenchymal stem cells (MSCs) is promising for stem cell therapy. We recently showed that bone morphogenetic protein 4 (BMP4) signalling involves the effect of cholesterol myristate on the proliferation of MSCs. The present study evaluated the effects of cholesterol myristate on the apoptosis of MSCs and the inhibitor of differentiation (Id1), target gene of BMP4 signalling. MSCs transfected by the Id1 promoter reporter construct, choles...
8 CitationsSource
#1Dongfeng Chen (NU: Nanjing University)H-Index: 22
#1Dong-Feng Chen (NU: Nanjing University)H-Index: 4
Last. Zi-Chun Hua (NU: Nanjing University)H-Index: 4
view all 9 authors...
Abstract To identify small molecules that induce dopaminergic neurons from neural stem cells (NSCs) is promising for therapy of Parkinson's disease. Here we report the results of analyzing structurally related steroids in traditional Chinese medicine to identify agents that enhance dopaminergic differentiation of NSCs. Using P19 cells transfected by tyrosine hydroxylase (TH) promoter reporter construct, (+)-Cholesten-3-one with carbonyl, but not cholesterol and cholesterol myristate can effectiv...
7 CitationsSource
#1Dongfeng Chen (NU: Nanjing University)H-Index: 22
#1Dong-Feng Chen (NU: Nanjing University)H-Index: 4
Last. Zi-Chun HuaH-Index: 4
view all 9 authors...
Abstract Buzhong Yiqi decoction (BYD) is a well-known ancient tonic prescription in traditional Chinese medicine (TCM). The purpose of this study is to identify active components of BYD involved in promoting proliferation of mesenchymal stem cells (MSCs) and to investigate its mechanism. BYD was extracted with petroleum ether, ethanol, and water. Evidence provided by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, bromodeoxyuridine, proliferation cell nuclear antigen immunoreactivi...
24 CitationsSource
#1Dongfeng Chen (GU: Guangzhou University)H-Index: 22
#1Dong-Feng Chen (GU: Guangzhou University)H-Index: 4
Last. Zi-Chun Hua (NU: Nanjing University)H-Index: 4
view all 11 authors...
Abstract We recently identified that cholesterol myristate in traditional Chinese medicine (TCM) is the active compound that increases proliferation of mesenchymal stem cell (MSCs). The present study is further to determine what signal pathway involves in effect of cholesterol myristate. Reverse transcription-PCR, Western blot and ELISA analysis show that cholesterol myristate increases the release of bone morphogenetic protein 4 (BMP4) from MSCs and the expression in the intracellular levels of...
4 CitationsSource
#1Wei-wei Mao (SCUT: South China University of Technology)H-Index: 1
#2Tingting Wang (SCUT: South China University of Technology)H-Index: 13
Last. Jiangang Shen (HKU: University of Hong Kong)H-Index: 50
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Abstract A library of novel 5-hydroxycoumarin and pyranocoumarin derivatives was constructed via silica sulfuric acid-catalyzed pechmann reaction and Pd(0)-catalyzed suzuki coupling in tandem, and their antiproliferative activities against breast cancer cells MCF-7 and MDA-MB-231 were evaluated. The results showed that compounds such as 6b , 6d , 6h , and 6k possess significant antiproliferative activity against MCF-7 cell line with the IC 50 values of 7.2, 5.3, 3.3, and 6.5 μM, respectively.
27 CitationsSource
#1He-Ping Zeng (SCUT: South China University of Technology)H-Index: 8
#1Heping Zeng (SCUT: South China University of Technology)H-Index: 13
Last. Jiangang Shen (HKU: University of Hong Kong)H-Index: 50
view all 6 authors...
Abstract Si-wu decoction (SDE), a classic prescription in Traditional Chinese Medicine, has been used for the treatment of a variety of anaemia in China for centuries. In order to explore the scientific basis of the formula, we investigated the relationship between its chemical components and proliferation-promoting effects on rat marrow-derived mesenchymal stem cells (MSCs). Twenty (F-1–F-20) components were obtained and their proliferation-promoting effects on MSCs were investigated. The resul...
11 CitationsSource
#1Yue-Hua ZhangH-Index: 1
#2He-Ping ZengH-Index: 8
Last. Dongfeng ChenH-Index: 22
view all 0 authors...
To investigate the components in Plastrum Testudinis which have effects on the proliferation of rat bone marrow mesenchymal stem cells( bMMSCs), the active parts of plastrum testudinis which can promote proliferation of rat mesenchymal stem cells were extracted by petroleum aether. The activities of inducing the proliferation of bMMSCs were studied by MTT assay and flow cytometry. The chemical components of extraction were analyzed by GC-MS. The results showed that the petroleum aether extractio...
1 CitationsSource