Florent Poulhes
Genetic enhancementOphthalmologyGene silencingGene deliveryGenetically modified mouseAntigenTransporterTranslation (biology)High-content screeningMagnetofectionNeuroscienceNanotechnologyRepliconChemistryCell typeMaterials scienceIn vivoSmall interfering RNAVirusEx vivoInflammationRetinaDrug deliveryRetinalRetinal degenerationRetinitis pigmentosaBardet–Biedl syndromeMicroglial cellRetinal DisorderNeedle freeCellular immunityNon invasiveOperations researchCentral nervous systemTransfectionNucleoproteinRNAMedicineBiologyMagnetic nanoparticlesImmune systemCell biology
8Publications
2H-index
29Citations
Publications 8
Newest
#1Marco BassettoH-Index: 1
#2Merve Sen (University of Tübingen)H-Index: 2
Last. Olivier ZelphatiH-Index: 14
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The prevalence of retinal disorders associated with visual impairment and blindness is increasing worldwide, while most of them remain without effective treatment. Pharmacological and molecular therapy development is hampered by the lack of effective drug delivery into the posterior segment of the eye. Among molecular approaches, RNA-interference (RNAi) features strong advantages, yet delivering it to the inner layer of the retina appears extremely challenging. To address this, we developed an o...
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#1Merve Sen (University of Tübingen)H-Index: 2
#2Marco BassettoH-Index: 1
Last. Blanca Arango-Gonzalez (University of Tübingen)H-Index: 18
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The use of synthetic RNA for research purposes as well as RNA-based therapy and vaccination has gained increasing importance. Given the anatomical seclusion of the eye, small interfering RNA (siRNA)-induced gene silencing bears great potential for targeted reduction of pathological gene expression that may allow rational treatment of chronic eye diseases in the future. However, there is yet an unmet need for techniques providing safe and efficient siRNA delivery to the retina. We used magnetic n...
2 CitationsSource
#1Marco BassettoH-Index: 1
#2Daniel Roberto Ajoy Moreno (UDS: University of Strasbourg)
Last. Olivier ZelphatiH-Index: 14
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#1Daniel Roberto Ajoy Moreno (UDS: University of Strasbourg)
Last. Vincent Marion (UDS: University of Strasbourg)H-Index: 19
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#1C. Di Scala (AMU: Aix-Marseille University)
#2M. Tessier (AMU: Aix-Marseille University)
Last. Christophe Pellegrino (AMU: Aix-Marseille University)H-Index: 21
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Abstract Background Gene delivery within the central nervous system at postnatal age is one of the most challenging tasks in neuroscience and currently only a few effective methods are available. Comparison with existing methods For postnatal central nervous system cells, viral approaches are commonly used for genetic engineering but they face several biosafety requirements for production and use making them less accessible to the community. Conversely, lipid-based methods are widely used in cel...
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Advances in RNA technology during the past two decades have led to the construction of replication-competent RNA, termed replicons, RepRNA, or self-amplifying mRNA, with high potential for vaccine applications. Cytosolic delivery is essential for their translation and self-replication, without infectious progeny generation, providing high levels of antigen expression for inducing humoral and cellular immunity. Synthetic nanoparticle-based delivery vehicles can both protect the RNA molecules and ...
17 CitationsSource
#1Silke Smolders (Katholieke Universiteit Leuven)H-Index: 4
#2Sofie KesselsH-Index: 4
Last. Bert BrôneH-Index: 17
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We thank Petra Bex for helping with all experiments. Financial support for this research was granted by the UHasselt (BOF13N01, BOF16NI04), the Research Foundation of Flanders 7 (FWO G0A0513) and Rotary campaign “Hope in Head”.
10 CitationsSource
#1Sofie KesselsH-Index: 4
#2Silke SmoldersH-Index: 4
view all 7 authors...
We thank Petra Bex for helping with all experiments. Financial support for this research was granted by the UHasselt (BOF13N01, BOF16N104), the Research Foundation of Flanders 7 (FWO G0A0513) and Rotary campaign "Hope in Head".