M. Sharon Stack
University of Notre Dame
CancerPathologyDNA damageCellChemistryExtracellular matrixTissue microarrayTumor microenvironmentPeritoneal cavityMetastasisOvarian tumorOvarian cancerCancer researchMedicineCell cultureAtmospheric-pressure plasmaBiologyCell biologyCancer cell
63Publications
17H-index
720Citations
Publications 52
Newest
Proteases play a crucial role in the progression and metastasis of ovarian cancer. Pericellular protein degradation and fragmentation along with remodeling of the extracellular matrix (ECM) is accomplished by numerous proteases that are present in the ovarian tumor microenvironment. Several proteolytic processes have been linked to cancer progression, particularly those facilitated by the matrix metalloproteinase (MMP) family. These proteases have been linked to enhanced migratory ability, extra...
Source
#1Yueying Liu (ND: University of Notre Dame)H-Index: 16
#2Jing Yang (ND: University of Notre Dame)H-Index: 6
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 28 authors...
Abstract The majority of women with ovarian cancer are diagnosed with metastatic disease, therefore elucidating molecular events that contribute to successful metastatic dissemination may identify additional targets for therapeutic intervention and thereby positively impact survival. Using two human high grade serous ovarian cancer cell lines with inactive TP53 and multiple rounds of serial in vivo passaging, we generated sublines with significantly accelerated intra-peritoneal (IP) growth. Comp...
Source
#1Jing Yang (ND: University of Notre Dame)H-Index: 6
#2M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
2 CitationsSource
#1Marwa Asem (ND: University of Notre Dame)H-Index: 5
#2Allison Young (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 10 authors...
The majority of women with recurrent ovarian cancer (OvCa) develop malignant ascites with volumes that can reach > 2 L. The resulting elevation in intraperitoneal pressure (IPP), from normal values of 5 mmHg to as high as 22 mmHg, causes striking changes in the loading environment in the peritoneal cavity. The effect of ascites-induced changes in IPP on OvCa progression is largely unknown. Herein we model the functional consequences of ascites-induced compression on ovarian tumor cells and compo...
8 CitationsSource
#1Yuliya Klymenko (IU: Indiana University)H-Index: 8
#2Brandi Bos (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 8 authors...
Epithelial ovarian cancer (EOC) metastasis occurs by exfoliation of cells and multicellular aggregates (MCAs) from the tumor into the peritoneal cavity, adhesion to and retraction of peritoneal mesothelial cells and subsequent anchoring. Elevated levels of lysophosphatidic acid (LPA) have been linked to aberrant cell proliferation, oncogenesis, and metastasis. LPA disrupts junctional integrity and epithelial cohesion in vitro however, the fate of free-floating cells/MCAs and the response of host...
1 CitationsSource
#1Xu Han (ND: University of Notre Dame)H-Index: 3
#2J. Kapaldo (ND: University of Notre Dame)H-Index: 5
Last. Sylwia Ptasinska (ND: University of Notre Dame)H-Index: 31
view all 6 authors...
The effective clinical application of atmospheric pressure plasma jet (APPJ) treatments requires a well-founded methodology that can describe the interactions between the plasma jet and a treated sample and the temporal and spatial changes that result from the treatment. In this study, we developed a large-scale image analysis method to identify the cell-cycle stage and quantify damage to nuclear DNA in single cells. The method was then tested and used to examine spatio-temporal distributions of...
2 CitationsSource
#1Marwa Asem (ND: University of Notre Dame)H-Index: 5
#2Allison Young (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 14 authors...
The non-canonical Wnt ligand Wnt5a is found in high concentrations in ascites of women with ovarian cancer. In this study, we elucidated the role of Wnt5a in ovarian cancer metastasis. Wnt5a promoted ovarian tumor cell adhesion to peritoneal mesothelial cells as well as migration and invasion, leading to colonization of peritoneal explants. Host components of the ovarian tumor microenvironment, notably peritoneal mesothelial cells and visceral adipose, secreted Wnt5a. Conditional knockout of hos...
6 CitationsSource
#1M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
#2Kenneth P. Nephew (IU: Indiana University)H-Index: 10
Last. Anirban K. Mitra (IU: Indiana University)H-Index: 19
view all 4 authors...
The Special Issue on high grade serous ovarian cancer (HGSOC) and the contribution of the tumor micro-environment (TME) consisted of reviews contributed by leaders in the ovarian cancer (OC) field. [...]
9 CitationsSource
#1Yuliya Klymenko (ND: University of Notre Dame)H-Index: 8
#2Rebecca B. Wates (KU: University of Kansas)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 10 authors...
ABSTRACT Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy. EOC dissemination is predominantly via direct extension of cells and multicellular aggregates (MCAs) into the peritoneal cavity, which adhere to and induce retraction of peritoneal mesothelium and proliferate in the submesothelial matrix to generate metastatic lesions. Metastasis is facilitated by the accumulation of malignant ascites (500 ml to >2 l), resulting in physical discomfort and abdominal distension, ...
9 CitationsSource
#1Jessica Tang (IU: Indiana University)H-Index: 7
#2Nicholas Pulliam (IU: Indiana University)H-Index: 5
Last. Kenneth P. NephewH-Index: 57
view all 10 authors...
Ovarian cancer (OC) cells frequently metastasize to the omentum and adipocytes play a significant role in ovarian tumor progression. Therapeutic interventions targeting aberrant DNA methylation in ovarian tumors have shown promise in the clinic but the effects of epigenetic therapy on the tumor microenvironment are understudied. Here, we examined the effect of adipocytes on OC cell behavior in culture and impact of targeting DNA methylation in adipocytes on OC metastasis. The presence of adipocy...
7 CitationsSource