Luiz C. Salay
State University of Santa Cruz
PoloxamerMicelleAnalytical chemistryBiological membraneBiophysicsAdsorptionSolubilityFourier transform infrared spectroscopyOrganic chemistryAqueous solutionChemistryMaterials sciencePhospholipidPeptideLipid bilayerMembraneChemical engineeringCarbon nanotubeBiochemistryMonolayer
26Publications
9H-index
230Citations
Publications 25
Newest
Saponins are bioactive compounds belonging to the secondary metabolism of plants widely used for their beneficial actions to human health. In this work the association of the saponin from Glycyrrhiza glabra with a matrix of multi-walled carbon nanotubes was promoted to obtain a composite material with improved functional characteristics. For this investigation, chemically modified electrodes (CME) based on carbon paste were developed. Firstly, a carbon paste electrode (CPE) modified with the add...
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#1Analu Rocha Costa (State University of Santa Cruz)H-Index: 2
#2Rodrigo Novais França (State University of Santa Cruz)
Last. Rodrigo Luis Silva Ribeiro Santos (State University of Santa Cruz)H-Index: 2
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Abstract Nitrogen and sulfur-containing heterocyclic compounds as pyrazole and dithiocarbazate have historically been reported as sources of therapeutic agents. This work reports by fluorescence measurements the colloid phenomena between the 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) and Pluronic F127, a copolymer that has a tendency to self-assemble into micelles in aqueous medium. The cytotoxicity of the DTC-based micellar system was evaluated in Swiss mice peritoneal macro...
2 CitationsSource
Last. Marcelo FrancoH-Index: 14
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Abstract In this work, an electrochemical approach for the quantitative detection of lactose in skimmed milk was proposed. To this end, a biosensor was developed from the immobilization of the enzyme lactase in the carbon nanotube matrix-carbon paste base. The paste from this association is deposited in the lower cavity of the working electrode, which together with the reference and auxiliary electrodes form the electrochemical cell operated from a potentiostat. The sensitivity and detection lim...
1 CitationsSource
#1Natielle Cachoeira Dotivo (UCSC: University of California, Santa Cruz)H-Index: 2
#2Rachel Passos Rezende (UCSC: University of California, Santa Cruz)H-Index: 16
Last. João Carlos Teixeira Dias (UCSC: University of California, Santa Cruz)H-Index: 14
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Traditional therapy for malignant neoplasms involving surgical procedures, radiotherapy and chemotherapy aims to kill neoplastic cells, but also affects normal cells. Therefore, exogenous proteases are the target of studies in cancer therapy, as they have been shown to be effective in suppressing tumors and reducing metastases. Pluronic F127 (F127) is a copolymer of amphiphilic blocks that has shown significant potential for drug administration, as it is capable of incorporating hydrophobic drug...
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Last. Marcelo Franco (UCSC: University of California, Santa Cruz)H-Index: 14
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Abstract The innovative aspect of nanoparticles takes on an important role in the advancement of research on nanostructured materials. The objective of the present study was to carry out an investigation of the interactions between lactase enzyme and carbon nanotubes applied to the elaboration of a chemically modified carbon paste electrode. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray...
4 CitationsSource
Last. Marcelo Franco (UCSC: University of California, Santa Cruz)H-Index: 14
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The variety of products derived from milk, without or with lactose, encourages the development of more effective analytical techniques that can be applied to the quality control of both the production line and the final products. Thus, in this work an efficient and minimally invasive method for the detection of lactose was proposed, using a biosensor containing the enzyme lactase (LAC) immobilised on carbon nanotubes (CNTs) that, when reacting with lactose, emit an electrochemical signal. This b...
7 CitationsSource
#1Rosemaire S. S. de Oliveira (UCSC: University of California, Santa Cruz)H-Index: 1
#2Nélida Simona Marin Huachaca (UCSC: University of California, Santa Cruz)H-Index: 2
Last. Luiz C. Salay (UCSC: University of California, Santa Cruz)H-Index: 9
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Nanotechnology is important for the development of new materials capable to improve the development of novel drug delivery systems. Such systems may result in more efficient therapeutic effects with reduced toxicity and extended time in the organism and increase in bioavailability, biocompatibility, and solubility of drugs. Here it was investigated the interaction between the triblock copolymer Pluronic F127 (F127) and a natural bioactive amphiphilic saponin (SAP), extracted from Glycyrrhiza gla...
6 CitationsSource
#1José Carlos Bozelli (USP: University of São Paulo)H-Index: 10
#2Luiz C. Salay (USP: University of São Paulo)H-Index: 9
Last. Shirley Schreier (USP: University of São Paulo)H-Index: 37
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Abstract A strategy that has been gaining increased application for the study of the conformation, dynamics, orientation, and physicochemical properties of peptides is labeling with the paramagnetic amino acid TOAC. This approach was used to gain a deeper understanding on the mechanism of action of the antimicrobial peptide tritrpticin (TRP3). TRP3 was labeled with TOAC at the N-terminus (prior to V1, TOAC0-TRP3) or internally (replacing P5, TOAC5-TRP3). Functional studies showed that labeling l...
4 CitationsSource
#1Luiz C. Salay (UCSC: University of California, Santa Cruz)H-Index: 9
#2Elielma A. Prazeres (UCSC: University of California, Santa Cruz)H-Index: 1
Last. Eloi Feitosa (UNESP: Sao Paulo State University)H-Index: 24
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Triblock copolymers, such as Pluronic F127 (F127), are pharmaceutically important amphiphilic compounds that self-assemble in aqueous solution either as discrete or entangled micelles, depending on their concentration and temperature, which may function as drug delivery vehicle. Herein, we have synthesized the antimicrobial peptide tritrpticin (TRP3), a tryptophan (Trp)- and arginine (Arg)-rich peptide, sequence VRRFPWWWPFLRR, with a broad spectrum of action against bacteria and fungi, to invest...
4 CitationsSource
#1Tharcilla Braz Alves Pessoa (UEFS: State University of Feira de Santana)H-Index: 3
#2Rachel Passos Rezende (State University of Santa Cruz)
Last. João Carlos Teixeira Dias (UCSC: University of California, Santa Cruz)H-Index: 14
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Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease-producing clone from a metagenomic library derived from mangrove sediment. The protease was purified by ammonium sulphate precipitation and gel filtration chromatography, with a yield of 77.27% and a specific activity of 8.57 U μg−...
12 CitationsSource