Wenjie Sheng
Nanjing University
PronucleusEndocrinologyMolecular biologyAndrologyDNA demethylationEmbryoDemethylationIn vitroEstrogenOvaryCuscuta chinensis5-HydroxymethylcytosineIcariinMTT assayMale pronucleusSteroid hormone secretionFemale pronucleusBlotBiologyHuman fertilization
2Publications
1H-index
8Citations
Publications 2
Newest
#1Xiaowei Nie (NU: Nanjing University)H-Index: 1
#2Wenjie Sheng (NU: Nanjing University)H-Index: 1
Last. Yong Tan (NU: Nanjing University)H-Index: 1
view all 6 authors...
Abstract Aim of the study This study was designed to investigate the effect of different concentrations of Hyperin and Icariin (ICA)on proliferation and the secretion of estrogen (E2), and progesterone (P) in granulosa cells, and to explore the effect of Hyperin and Icariin on the expression of CYP17 and CYP19 . Materials and methods Rat ovary granulosa cells were cultured in vitro and treated with different concentrations of Hyperin and Icariin. The proliferation of ovarian granulosa cells was ...
7 CitationsSource
#1Xiaowei Nie (Nanjing Medical University)H-Index: 10
#2Qiang Liu (Shaanxi University of Chinese Medicine)H-Index: 1
Last. Rongfeng Li (Nanjing Medical University)H-Index: 6
view all 13 authors...
Abstract Recent studies in mice showed that the Ten-eleven translocation Enzymes (TET) family is involved in the active DNA demethylation. The isotype TET-3 is responsible for the conversion of 5mc (5-methylcytosine) to 5hmc (5-hydroxymethylcytosine) at the pronuclear stages of mouse embryo. This study was performed to investigate the pattern of methylation change and the role of TET family in the demethylation process of porcine in-vitro fertilization (IVF) and somatic cell nuclear transfer (SC...
2 CitationsSource