Wenwen Gao
Jilin University
Messenger RNAGene silencingInternal medicinePeroxisomeDownregulation and upregulationMicrosomal triglyceride transfer proteinEndocrinologyUnfolded protein responseLipolysismicroRNACellWhite adipose tissueEndoplasmic reticulumProinflammatory cytokineEndogenyReceptorAdipose tissueHepatic stellate cellTumor necrosis factor alphaChemistryIn vitroGluconeogenesisLipid dropletAdipocyteHepatocyteLiver injuryKetosisType 2 diabetesGlutamate dehydrogenaseInsulin resistanceGlycogenTunicamycinFatty liverATF6SteatohepatitisNEFAPropionateLipoproteinHormone-sensitive lipaseXBP1MitochondrionSterol Regulatory Element Binding Protein 1Liver functionGlucose homeostasisKetone bodiesDiacylglycerol kinaseLDL receptorLipotoxicityLipid metabolismFatty acid synthaseReactive oxygen speciesSecretionViability assayTransfectionApolipoprotein EOxidative phosphorylationApolipoprotein BIntracellularVery low-density lipoproteinSterol regulatory element-binding proteinBiology
9Publications
5H-index
92Citations
Publications 9
Newest
#1Zhen Shi (JLU: Jilin University)H-Index: 9
#2Yuxiang Song (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 28
view all 12 authors...
Disruption of endoplasmic reticulum (ER) homeostasis, a condition termed "ER stress," contributes to the development of liver injury in nonruminants. Because liver injury is a prominent pathological feature associated with overproduction of ketone bodies in dairy cows with ketosis, understanding the ER stress state and its functional consequences on liver injury is of particular interest. Here, 30 multiparous cows (within 3 wk postpartum) classified based on blood β-hydroxybutyrate (BHB) as heal...
Source
#1Wenwen Gao (JLU: Jilin University)H-Index: 5
#2Zhiyuan Fang (JLU: Jilin University)H-Index: 5
Last. Xiliang Du (JLU: Jilin University)H-Index: 12
view all 17 authors...
Negative energy balance-induced high blood concentrations of free fatty acids during the early postpartum period in dairy cows is a major cause of liver injury. Cows in severe negative energy balance often have suboptimal intakes of feed, which contributes to shortfalls in production of ruminal propionate and circulating glucose. Although increasing propionate production by the rumen through feed additives such as propylene glycol is effective in helping cows alleviate the shortfall in dietary e...
Source
#1Fan Minghe (JLU: Jilin University)
#2Xiliang Du (JLU: Jilin University)H-Index: 12
Last. Wenwen Gao (JLU: Jilin University)H-Index: 5
view all 16 authors...
ABSTRACT Dairy cows with ketosis exhibit signs of pronounced adipose tissue lipolysis and systemic inflammation, both of which exacerbate this metabolic disorder. In nonruminants, CIDEC plays a pivotal role in the formation of large unilocular lipid droplets. The present study aimed to ascertain the role of CIDEC in the lipolytic and inflammatory response of white adipose tissue (WAT) in vivo and in vitro. Subcutaneous adipose tissue and blood samples were collected from 15 healthy cows (blood β...
Source
#1Xiliang Du (JLU: Jilin University)H-Index: 12
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Guowen LiuH-Index: 28
view all 11 authors...
: Insulin resistance is often characterized as the most critical factor contributing to the development of (T2D) type 2 diabetes. MicroRNAs (miRNAs) are endogenous non-coding short single-stranded RNAs that function as negative regulators in many physiological and pathological processes. The objective of this study was to evaluate the roles of miR-125b in the regulation of insulin sensitivity in hepatocytes. We found that hepatic miR-125b levels were significantly increased in the patients with ...
6 CitationsSource
#1Wenwen Gao (JLU: Jilin University)H-Index: 5
#2Xiliang Du (JLU: Jilin University)H-Index: 12
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 9 authors...
: The aim of this study was to investigate the changes in hepatic oxidative phosphorylation (OXPHOS) complexes (COs) in patients and cows with non-alcoholic steatohepatitis (NASH) and to investigate the mechanism that links mitochondrial dysfunction and hepatic insulin resistance induced by non-esterified fatty acids (NEFAs). Patients and cows with NASH displayed high blood NEFAs, TNF-α and IL-6 concentrations, mitochondrial dysfunction and insulin resistance. The protein levels of peroxisome pr...
22 CitationsSource
#1Xiliang Du (JLU: Jilin University)H-Index: 12
#2Yuchen Yang (JLU: Jilin University)H-Index: 4
Last. Guowen LiuH-Index: 28
view all 14 authors...
// Xiliang Du 1 , Yuchen Yang 1 , Chuang Xu 2 , Zhicheng Peng 1 , Min Zhang 1 , Lin Lei 1 , Wenwen Gao 1 , Yuhao Dong 1 , Zhen Shi 1 , Xudong Sun 1 , Zhe Wang 1 , Xiaobing Li 1 , Xinwei Li 1 and Guowen Liu 1 1 Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China 2 College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China Correspondene to: Xinwei Li, email: lixinwei100@...
22 CitationsSource
#1Lin Lei (JLU: Jilin University)H-Index: 6
#2Yiwei Zhu (JLU: Jilin University)H-Index: 9
Last. Guowen Liu (JLU: Jilin University)H-Index: 28
view all 11 authors...
Abstract Alpha-lipoic acid (ALA) has been reported to have beneficial effects for improving insulin sensitivity. However, the underlying molecular mechanism of the beneficial effects remains poorly understood. Endoplasmic reticulum (ER) stress and mitochondrial dysfunction are considered causal factors that induce insulin resistance. In this study, we investigated the effect of ALA on the modulation of insulin resistance in ER-stressed HepG2 cells, and we explored the potential mechanism of this...
17 CitationsSource
#1Qinghua Deng (JLU: Jilin University)H-Index: 8
Last. Gang Liu (JLU: Jilin University)H-Index: 25
view all 14 authors...
Summary β-Hydroxybutyricacid (BHBA) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low-density lipoprotein (VLDL) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA. We found that BHBA treatment upregulated the mRNA and ...
Source
#1Min ZhangH-Index: 6
#2Shiqi ZhangH-Index: 2
Last. Xinwei LiH-Index: 23
view all 10 authors...
Background/Aims: In dairy cows, β-hydroxybutyrate (BHBA) is utilized as precursors of de novo synthesized fatty acids in mammary gland. Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration. Sterol regulatory element-binding protein1 (SREBP1) and cell death-inducing DNA fragmentation factor-alpha-like effector α (Cidea) play crucial roles in lipid synthesis. Therefore, we hypothesized that BHBA could stimulate SREBP1/Ci...
18 CitationsSource