Julian Schumacher
RWTH Aachen University
Downregulation and upregulationTransactivationCell migrationPathologyCellMolecular biologyImmunologyInflammationLungMatrigelCytokineADAM10ADAM8Lung injuryCancer researchMetalloproteinaseCell growthSyndecan 1MedicineA549 cellBiologyCell biology
16Publications
8H-index
233Citations
Publications 16
Newest
#1Julian SchumacherH-Index: 8
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#1Daniela Dreymueller (RWTH Aachen University)H-Index: 16
#2Jessica Pruessmeyer (RWTH Aachen University)H-Index: 14
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
view all 9 authors...
Alveolar leukocyte recruitment is a hallmark of acute lung inflammation and involves transmigration of leukocytes through endothelial and epithelial layers. The disintegrin and metalloproteinase (ADAM) 8 is expressed on human isolated leukocytic cells and can be further upregulated on cultured endothelial and epithelial cells by proinflammatory cytokines. By shRNA mediated knockdown we show that leukocytic ADAM8 is required on monocytic THP-1 cells for chemokine-induced chemotaxis as well as tra...
15 CitationsSource
#1Andrea Koenen (RWTH Aachen University)H-Index: 2
#2Aaron Babendreyer (RWTH Aachen University)H-Index: 8
Last. Daniela Dreymueller (RWTH Aachen University)H-Index: 16
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The CXC-chemokine receptor 6 (CXCR6) is a class A GTP-binding protein-coupled receptor (GPCRs) that mediates adhesion of leukocytes by interacting with the transmembrane cell surface-expressed chemokine ligand 16 (CXCL16), and also regulates leukocyte migration by interacting with the soluble shed variant of CXCL16. In contrast to virtually all other chemokine receptors with chemotactic activity, CXCR6 carries a DRF motif instead of the typical DRY motif as a key element in receptor activation a...
13 CitationsSource
#1Esther Groth (RWTH Aachen University)H-Index: 6
#2Jessica Pruessmeyer (RWTH Aachen University)H-Index: 14
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
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Abstract By mediating proteolytic shedding on the cell surface the disintegrin and metalloproteinases ADAM10 and ADAM17 function as critical regulators of growth factors, cytokines and adhesion molecules. We here report that stimulation of lung epithelial A549 tumor cells with phorbol-12-myristate-13-acetate (PMA) leads to the downregulation of the surface expressed mature form of ADAM17 without affecting ADAM10 expression. This reduction could not be sufficiently explained by metalloproteinase-...
33 CitationsSource
#1Tobias Pasqualon (RWTH Aachen University)H-Index: 7
#2Jessica Pruessmeyer (RWTH Aachen University)H-Index: 14
Last. Andreas LudwigH-Index: 63
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// Tobias Pasqualon 1 , Jessica Pruessmeyer 1 , Vera Jankowski 2 , Aaron Babendreyer 1 , Esther Groth 1 , Julian Schumacher 1 , Andrea Koenen 1 , Sarah Weidenfeld 1 , Nicole Schwarz 3 , Bernd Denecke 4 , Holger Jahr 5 , Daniela Dreymueller 1 , Joachim Jankowski 2 , Andreas Ludwig 1 1 Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany 2 Institute of Molecular Cardiovascular Research, RWTH Aachen University, Aachen, Germany 3 Institute of Molecular and Cellular Anato...
23 CitationsSource
Source
#1Tobias Pasqualon (RWTH Aachen University)H-Index: 7
#2Aaron Babendreyer (RWTH Aachen University)H-Index: 8
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 35
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Syndecan-1 is a heparan sulphate proteoglycan expressed on the cell surface and shed by epithelial lung tumors. We here demonstrate that syndecan-1 is critically involved in lung tumor growth and metastasis in vitro and vivo and investigate the regulatory role of its cellular cleavage fragments. Shedding of syndecan-1 from the cell surface generates a transmembrane C-terminal fragment (tCTF), which can be further processed by γ-secretase into a cytoplasmic fragment (cCTF). Scratch-induced wound ...
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#1Tobias Pasqualon (RWTH Aachen University)H-Index: 7
#2Jessica Pruessmeyer (RWTH Aachen University)H-Index: 14
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
view all 12 authors...
Syndecan-1 is a heparan sulfate proteoglycan expressed by endothelial and epithelial cells and involved in wound healing and tumor growth. Surface-expressed syndecan-1 undergoes proteolytic shedding leading to the release of the soluble N-terminal ectodomain from a transmembrane C-terminal fragment (tCTF). We show that the disintegrin and metalloproteinase (ADAM) 17 generates a syndecan-1 tCTF, which can then undergo further intra-membrane proteolysis by γ-secretase. Scratch-induced wound closur...
25 CitationsSource