Mariel Donzeau
University of Strasbourg
Modular designPolyacrylamide gel electrophoresisPromoterProtein subunitGeneColloidal goldCytotoxicityConjugated systemImmunotoxinCyclin DPhosphorylationFusion proteinBiophysicsResponse elementCas9General transcription factorAntigenMolecular biologyLeucine zipperVirologyAntibodyCyclin ASUMO proteinTranscription factor II DCyclin-dependent kinase complexCovalent bondG1/S transitionKinaseChemistryComplementationMaterials scienceAlternative splicingConjugateActivating transcription factor 2ImmunologyMacromoleculeCombinatorial chemistryVaccinationPlasmidTranscriptional regulationMultiple myelomaB-cell lymphomaToxinImmunotherapyB cellTetramerTCEPEpidermal growth factor receptorCetuximabBifunctionalImmunogenicityCyclin A2ParaproteinsProliferating cell nuclear antigenClinical trialEscherichia coliBiochemistryMedicineIdiotypeDNA polymerase deltaActivating transcription factorChromatinTranscription factorBiologyImmune systemCyclin ECell biologyCancer cellBinding selectivityProtein kinase ACyclin BCRISPR
9Publications
5H-index
65Citations
Publications 9
Newest
#1Audrey Stoessel (UDS: University of Strasbourg)H-Index: 5
#2Nadja Groysbeck (UDS: University of Strasbourg)H-Index: 2
Last. Mariel Donzeau (UDS: University of Strasbourg)H-Index: 5
view all 16 authors...
Immunotoxins are emerging candidates for cancer therapeutics. These biomolecules consist of a cell-targeting protein combined to a polypeptide toxin. Associations of both entities can be achieved either chemically by covalent bonds or genetically creating fusion proteins. However, chemical agents can affect the activity and/or stability of the conjugate proteins, and additional purification steps are often required to isolate the final conjugate from unwanted byproducts. As for fusion proteins, ...
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#1Marc Vigneron (UDS: University of Strasbourg)H-Index: 17
#2Frank Dietsch (UDS: University of Strasbourg)H-Index: 1
Last. Mariel Donzeau (UDS: University of Strasbourg)H-Index: 5
view all 9 authors...
Monitoring the assembly of macromolecules to design entities with novel properties can be achieved either chemically creating covalent bonds or by noncovalent connections using appropriate structural motifs. In this report, two self-associating peptides (named K3 and E3) that originate from p53 tetramerization domain were developed as tools for highly specific and noncovalent heterotetramerization of two biomolecules. The pairing/coupling preferences of K3 and E3 were first evaluated by molecula...
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#1Nadja Groysbeck (UDS: University of Strasbourg)H-Index: 2
#2Audrey Stoessel (UDS: University of Strasbourg)H-Index: 5
Last. Guy Zuber (UDS: University of Strasbourg)H-Index: 26
view all 9 authors...
: Therapeutic monoclonal antibodies benefit to patients and the conjugation to gold nanoparticles (AuNPs) might bring additional activities to these macromolecules. However, the behavior of the conjugate will largely depend on the bulkiness of the AuNP and small sizes are moreover preferable for diffusion. Water-soluble thiolate-protected AuNPs having diameters of 2-3 nm can be synthesized with narrow polydispersity and can selectively react with incoming organic thiols via a SN2-like mechanism....
8 CitationsSource
#1Frank DietschH-Index: 1
#2Mariel DonzeauH-Index: 5
Last. Marc VigneronH-Index: 17
view all 6 authors...
Here we developed a complementation method for the study of essential genes in live human cells using the CRISPR/Cas9 system. Proteins encoded by essential genes were expressed using a derivative of the pCEP4 compensating plasmid in combination with Cas9 endonuclease targeting of the chromosomal genes. We show that this strategy can be applied to essential genes, such as those coding for proliferating cell nuclear antigen (PCNA) and DNA polymerase delta subunit 2 (POLD2). As demonstrated for the...
1 CitationsSource
#1Etienne Schaeffer (UDS: University of Strasbourg)H-Index: 3
#2Marc Vigneron (UDS: University of Strasbourg)H-Index: 17
Last. Mariel Donzeau (UDS: University of Strasbourg)H-Index: 5
view all 6 authors...
The transcription factor ATF7 undergoes multiple post-translational modifications, each of which has distinct effects upon ATF7 function. Here, we show that ATF7 phosphorylation on residue Thr112 exclusively occurs during mitosis, and that ATF7 is excluded from the condensed chromatin. Both processes are CDK1/cyclin B dependent. Using a transduced neutralizing monoclonal antibody directed against the Thr112 epitope in living cells, we could demonstrate that Thr112 phosphorylation protects endoge...
3 CitationsSource
#1Tim Roehnisch (LMU: Ludwig Maximilian University of Munich)H-Index: 4
#2Cornelia Then (LMU: Ludwig Maximilian University of Munich)H-Index: 13
Last. Fuat Oduncu (LMU: Ludwig Maximilian University of Munich)H-Index: 20
view all 10 authors...
Background: Multiple myeloma is characterized by clonal expansion of B cells producing monoclonal immunoglobulins or fragments thereof, which can be detected in the serum and/or urine and are ideal target antigens for patient-specific immunotherapies. Methods: Using phage particles as immunological carriers, we employed a novel chemically linked idiotype vaccine in a clinical phase I/II trial including 15 patients with advanced multiple myeloma. Vaccines composed of purified paraproteins linked ...
13 CitationsSource
#1Tim Roehnisch (LMU: Ludwig Maximilian University of Munich)H-Index: 4
#2Cornelia Then (LMU: Ludwig Maximilian University of Munich)H-Index: 13
Last. Fuat Oduncu (LMU: Ludwig Maximilian University of Munich)H-Index: 20
view all 9 authors...
Background B cell malignancies are characterized by clonal expansion of B cells expressing tumor-specific idiotypes on their surface. These idiotypes are ideal target antigens for an individualized immunotherapy. However, previous idiotype vaccines mostly lacked efficiency due to a low immunogenicity of the idiotype. The objective of the present study was the determination of the feasibility, safety and immunogenicity of a novel chemically linked phage idiotype vaccine.
11 CitationsSource
#1Jessica Diring (UDS: University of Strasbourg)H-Index: 2
#2Barbara Camuzeaux (UDS: University of Strasbourg)H-Index: 3
Last. Bruno Chatton (UDS: University of Strasbourg)H-Index: 25
view all 7 authors...
Alternative splicing and post-translational modifications are processes that give rise to the complexity of the proteome. The nuclear ATF7 and ATF2 (activating transcription factor) are structurally homologous leucine zipper transcription factors encoded by distinct genes. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MA...
12 CitationsSource
#1Barbara Camuzeaux (UDS: University of Strasbourg)H-Index: 3
#2Jessica Diring (UDS: University of Strasbourg)H-Index: 2
Last. Bruno Chatton (UDS: University of Strasbourg)H-Index: 25
view all 8 authors...
The ubiquitous activating transcription factor (ATF) 7 binds as a homodimer to the cAMP response element/TPA response element motifs present in the promoters of its target genes. ATF7 is homologous to ATF2 and heterodimerizes with Jun or Fos proteins, modulating their DNA-binding specificities. We previously demonstrated that TAF12, a component of the TFIID general transcription factor, mediates ATF7 transcriptional activity through direct interactions between the two proteins. By contrast, ATF7...
15 CitationsSource