Ken Kikuchi
Kyoto Prefectural University of Medicine
Fusion geneCancerInternal medicinePathologyCellMolecular biologyPAX3ChemistryApoptosisImmunologyAlveolar rhabdomyosarcomaNeuroblastomaEmbryonal rhabdomyosarcomaMetastasisRhabdomyosarcomaCell cycleCancer researchCell growthMedicineCell cultureBiology
43Publications
14H-index
586Citations
Publications 42
Newest
#1Carla Regina (University Medical Center Freiburg)
#2Ebrahem Hamed (University Medical Center Freiburg)
Last. Ken Kikuchi (Kyoto Prefectural University of Medicine)H-Index: 14
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Rhabdomyosarcomas (RMS) are phenotypically and functionally heterogeneous. Both primary human RMS cultures and low-passage Myf6Cre,Pax3:Foxo1,p53 mouse RMS cell lines, which express the fusion oncoprotein Pax3:Foxo1 and lack the tumor suppressor Tp53 (Myf6Cre,Pax3:Foxo1,p53), exhibit marked heterogeneity in PAX3:FOXO1 (P3F) expression at the single cell level. In mouse RMS cells, P3F expression is directed by the Pax3 promoter and coupled to eYFP YFPlow/P3Flow mouse RMS cells included 87% G0/G1 ...
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#1Hiroshi Kubo (Kyoto Prefectural University of Medicine)H-Index: 1
#2Shigeki Yagyu (Kyoto Prefectural University of Medicine)H-Index: 14
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
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Abstract Ephrin type-B receptor 4 (EPHB4), expressed in tumors including rhabdomyosarcoma, is a suitable target for chimeric antigen receptor (CAR)-T cells. Ligand independent activation of EPHB4 causes cell proliferation and malignant transformation in rhabdomyosarcoma, whereas ligand-dependent stimulation of EPHB4 induces apoptosis in rhabdomyosarcoma. Therefore, we hypothesized that ligand-based EPHB4-specific CAR-T cells may kill rhabdomyosarcoma cells without stimulating downstream cell pro...
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#1Satoshi MiyagakiH-Index: 2
#2Ken KikuchiH-Index: 14
Last. Hajime HosoiH-Index: 33
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#1Yohei Sugimoto (Kyoto Prefectural University of Medicine)H-Index: 1
#2Yoshiki Katsumi (Kyoto Prefectural University of Medicine)H-Index: 8
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
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Rhabdoid tumor is an aggressive, early childhood tumor. Biallelic inactivation of the SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1)/integrase interactor 1 (INI1) gene is the only common genetic feature in rhabdoid tumors. Loss of SMARCB1 function results in downregulation of several tumor suppressor genes including p16, p21, and NOXA. The novel histone deacetylase inhibitor, OBP-801, induces p21 and has shown efficacy against various canc...
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#1Kazutaka Ouchi (Kyoto Prefectural University of Medicine)H-Index: 6
#2Mitsuru Miyachi (Kyoto Prefectural University of Medicine)H-Index: 11
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
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Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma. There are two subtypes, fusion gene-positive RMS (FP-RMS) and fusion gene-negative RMS (FN-RMS), depending on the presence of a fusion gene, either PAX3-FOXO1 or PAX7-FOXO1. These fusion genes are thought to be oncogenic drivers of FP-RMS. By contrast, the underlying mechanism of FN-RMS has not been thoroughly investigated. It has recently been shown that HMGA2 is specifically positive in pathological tissue from FN-RMS, bu...
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#1Ryoji Yoshida (Kumamoto University)H-Index: 15
#2Masashi Nagata (Kumamoto University)H-Index: 15
Last. Hideki Nakayama (Kumamoto University)H-Index: 24
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It has been reported that 20% of early-stage oral squamous cell carcinoma (OSCC) patients treated with surgery alone (SA) may exhibit postoperative relapse within 2-3 years and have poor prognoses. We aimed to determine the safety of S-1 adjuvant chemotherapy and the potential differences in the disease-free survival (DFS) between patients with T2N0 (stage II) OSCC treated with S-1 adjuvant therapy (S-1) and those treated with SA. This single-center retrospective cohort study was conducted at Ku...
1 CitationsSource
#1Tomoko Iehara (Kyoto Prefectural University of Medicine)H-Index: 21
#2Shigeki Yagyu (Kyoto Prefectural University of Medicine)H-Index: 14
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
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BACKGROUND: Our previous study reported a method for determining MYCN gene amplification (MNA) status using cell-free DNA in serum. We prospectively analyzed the serum MNA status using sera obtained before the initial diagnosis from patients with neuroblastoma and evaluated the utility of this method. METHODS: Eighty patients were enrolled in the study. The serum MYCN/NAGK ratio was assessed for all cases. RESULTS: Fifteen cases showed serum MNA, while 65 did not. Of the 80 total patients, tumor...
2 CitationsSource
#1Norio Nakagawa (Kyoto Prefectural University of Medicine)H-Index: 3
#2Ken Kikuchi (Kyoto Prefectural University of Medicine)H-Index: 14
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
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Abstract Precision medicine strategies for treating rhabdomyosarcoma (RMS), a childhood malignancy, have not been developed. We examined the effect of CH5126766, a potent selective dual RAF/MEK inhibitor, on RMS cell lines. Among the eleven cell lines studied, one NRAS and two HRAS mutated cell lines were detected. CH5126766 inhibited the proliferation and growth in all of the RAS-mutated RMS cell lines, while it induced G1 cell cycle arrest in two of them. G1 cell cycle arrest was accompanied b...
3 CitationsSource
#1Masakazu Yoneda (Kumamoto University)H-Index: 2
#2Ryuji Imamura (Kumamoto University)H-Index: 1
Last. Takahisa Imamura (Kumamoto University)H-Index: 27
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: Autoimmune diseases are caused by immune complex-induced activation of the complement system and subsequent inflammation. Recent studies have revealed an association between autoimmune diseases and worse survival in patients with cancer; however, the underlying mechanism is still unknown. The C5a-C5a receptor (C5aR) system has been shown to enhance cancer activity and recruit myeloid-derived suppressor cells (MDSCs) that suppress the anti-tumor immune response. The Arthus reaction is inflammat...
8 CitationsSource
#1Chihiro Tomoyasu (Kyoto Prefectural University of Medicine)H-Index: 3
#2Ken Kikuchi (Kyoto Prefectural University of Medicine)H-Index: 14
Last. Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
view all 9 authors...
: Rhabdomyosarcoma (RMS) is an aggressive pediatric cancer of musculoskeletal origin. Despite multidisciplinary approaches, such as surgical resection, irradiation, and intensive chemotherapy, adopted for its treatment, the prognosis of patients with high‑risk RMS remains poor. Thus, molecularly targeted therapies are required to improve patient survival and minimize side effects. Histone deacetylases (HDACs) modify transcription by deacetylation of the lysine residues in chromatin histone tails...
2 CitationsSource