Georgios Theocharidis
Beth Israel Deaconess Medical Center
CellTranscriptomeChemistryCell typeExtracellular matrixImmunologyBasement membraneMacrophage polarizationInflammationHuman skinDermisDiabetic footDiabetic foot ulcerWound healingFibroblastExtracellularCancer researchDiabetes mellitusMedicineCell biology
15Publications
5H-index
108Citations
Publications 15
Newest
#1Georgios Theocharidis (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 5
#1G. Theocharidis (Emory University)
Last. Manoj BhasinH-Index: 41
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To understand the diabetic wound healing microenvironment, we profiled 174,962 single cells from foot, forearm, and PBMCs using single-cell RNA sequencing (scRNASeq) approach. Our analysis shows enrichment of a unique population of fibroblasts overexpressing MMP1, MMP3, MMP11, HIF1A, CHI3L1, and TNFAIP6 genes and M1 macrophage polarization in the DFU patients with healing wounds. Further, scRNASeq of spatially separated samples from same patient and spatial transcriptomics (ST) revealed preferen...
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#1Avi Smith (Tufts University)H-Index: 10
#2Trishawna Watkins (Tufts University)
Last. Jonathan A. Garlick (Tufts University)H-Index: 32
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1 CitationsSource
#1Georgios Theocharidis (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 5
#2Dimitrios Baltzis (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 5
Last. Aristidis Veves (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 60
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Non-healing diabetic foot ulceration (DFU) is characterized by low grade chronic inflammation, both locally and systemically. We prospectively followed a group of DFU patients who either healed or developed non-healing chronic DFU. Serum and forearm skin analysis, both at protein expression and transcriptomic level, indicated that increased expression of factors such as IFNγ, VEGF and sVCAM-1 were associated with DFU healing. Furthermore, foot skin single-cell RNA-seq analysis showed multiple fi...
5 CitationsSource
#1Ana Tellechea (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 12
#2Sha Bai (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 3
Last. Aristidis Veves (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 60
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ABSTRACT Impaired wound healing in the diabetic foot is a major problem often leading to amputation. Mast cells have been shown to regulate wound healing in diabetes. We developed an indole-carboxamide type mast cell stabilizer, MCS-01, which proved to be an effective mast cell degranulation inhibitor in vitro and can be delivered topically for prolonged periods through controlled release by specifically designed alginate bandages. In diabetic mice, both pre- and post-wounding, topical MCS-01 ap...
13 CitationsSource
#1Georgios Theocharidis (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 5
#2Aristidis Veves (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 60
Abstract Lower extremity ulcerations represent a major complication in diabetes mellitus and involve multiple physiological factors that lead to impairment of wound healing. Neuropeptides are neuromodulators implicated in various processes including diabetic wound healing. Diabetes causes autonomic and small sensory nerve fibers neuropathy as well as inflammatory dysregulation, which manifest with decreased neuropeptide expression and a disproportion in pro- and anti- inflammatory cytokine respo...
5 CitationsSource
#1Georgios Theocharidis (QMUL: Queen Mary University of London)H-Index: 5
#2John T. Connelly (QMUL: Queen Mary University of London)H-Index: 27
The structure and function of the skin relies on the complex expression pattern and organisation of extracellular matrix macromolecules, of which collagens are a principal component. The fibrillar collagens, types I and III, constitute over 90% of the collagen content within the skin and are the major determinants of the strength and stiffness of the tissue. However, the minor collagens also play a crucial regulatory role in a variety of processes, including cell anchorage, matrix assembly, and ...
6 CitationsSource
#1Olga Kashpur (Tufts University)H-Index: 4
#2Avi Smith (Tufts University)H-Index: 10
Last. Jonathan A. Garlick (Tufts University)H-Index: 32
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Diabetic foot ulcers (DFUs) are a major complication of diabetes, and there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds. Induced pluripotent stem cells (iPSCs) offer a replenishing source of allogeneic and autologous cell types that may be beneficial to improve DFU wound-healing outcomes. However, the biologic potential of iPSC-derived cells to treat DFUs has not, to our knowledge, been investigated. Toward that goal, we have performed detai...
12 CitationsSource
#1M. GhettiH-Index: 1
#1Martina GhettiH-Index: 5
Last. Claire A. HigginsH-Index: 15
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#2Swati S. BhasinH-Index: 8
Last. Aristidis VevesH-Index: 60
view all 5 authors...
Fifteen percent of diabetes mellitus (DM) patients are expected to develop a diabetic foot ulcer (DFU) within their lifetime. DFUs represent an ever increasing burden for the healthcare system. Chronic wounds, lack the linear progression from one classical phase of healing to the next and are mainly characterized by the persistence of the inflammatory phase. To define the transcriptome landscape of healthy uninjured skin, DM and DFU skin, we performed transcriptome profiling on skin specimens fr...
2 CitationsSource
#1Martina Ghetti (UNIBO: University of Bologna)H-Index: 5
#1M. Ghetti (UNIBO: University of Bologna)H-Index: 1
Last. Claire A. Higgins (Imperial College London)H-Index: 15
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BACKGROUND: While several commercial dermoepidermal scaffolds can promote wound healing of the skin, the achievement of complete skin regeneration still represents a major challenge. OBJECTIVES: To perform biological characterization of self-assembled extracellular matrices (ECMs) from three different subpopulations of fibroblasts found in human skin: papillary fibroblasts (Pfi), reticular fibroblasts (Rfi) and dermal papilla fibroblasts (DPfi). METHODS: Fibroblast subpopulations were cultured w...
13 CitationsSource