Wen Fang Chen
Qingdao University
EndocrinologyDopaminergicReceptorEstrogen receptor alphaChemistryGrowth factorPars compactaEstrogenOsteoblastGenisteinMPTPSubstantia nigraMAPK/ERK pathwayCell growthSignal transductionEstrogen receptorBiologyEstrogen receptor betaProtein kinase APharmacology
13Publications
11H-index
546Citations
Publications 13
Newest
#1Lin Wu (Qingdao University)H-Index: 3
#2Zhong Rui Du (Qingdao University)H-Index: 2
Last. Wen Fang Chen (Qingdao University)H-Index: 11
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Flavonoids, the active components of Epimedii Genus, have been demonstrated to protect against osteoporosis, cardiovascular diseases and rheumatoid arthritis. The present study aimed to investigate the neuroprotective effects of total flavonoid (TF) fraction of Epimedium koreanum Nakai on dopaminergic neurons in the cellular and mice models of Parkinson's disease (PD). TF pretreatment could ameliorate the decrease of striatal dopamine (DA) content and the loss of tyrosine hydroxylase (TH)-immuno...
11 CitationsSource
#1Wen Fang Chen (Qingdao University)H-Index: 11
#2Lin Wu (Qingdao University)H-Index: 3
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
view all 8 authors...
Abstract Background Epimedium sagittatum is a traditional Chinese herb normally which is used to treat the osteoporosis, cardiovascular dysfunction, and to improve neurological and sexual function in China, Korea and Japan. Icariin is the major active ingredient in Epimedium sagittatum . In the present research, we examined the neuroprotective effects of icariin on dopaminergic neurons and the possible mechanisms in a mouse model of Parkinson's disease (PD). Methods Ovariectomized PD mice were t...
45 CitationsSource
#1Wen Fang Chen (Qingdao University)H-Index: 11
#2Li-Ping Zhou (Qingdao University)H-Index: 1
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
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Abstract Ginsenoside Rg1 is the main pharmacologically active compound of ginsenosides and has demonstrated pharmacological effects in the cardiovascular system, central nervous system and immune system. The involvement of insulin-like growth factor-I receptor (IGF-IR)-dependent pathway and estrogen receptor (ER)-dependent pathway in the biological effect of ginsenoside Rg1 have been demonstrated in our previous study. The present study tested the hypothesis that the protective effects of Rg1 ag...
19 CitationsSource
#1Wen Fang Chen (PolyU: Hong Kong Polytechnic University)H-Index: 11
#2Quan Gui GaoH-Index: 6
Last. Man Sau WongH-Index: 36
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OBJECTIVE: The present study was designed to determine whether ginsenoside Rg1 could exert selective estrogenic effects by using both cell lines and an animal model. METHODS: The endometrial Ishikawa cells and preosteoblastic MC3T3-E1 cells were treated with a different dose of Rg1. Immature CD-1 mice and ovariectomized (OVX) C57BL/6J mice were used to study the short-term and long-term estrogenic effects of Rg1, respectively. RESULTS: Rg1 significantly increased estrogen receptor-dependent alka...
11 CitationsSource
#1Quan Gui Gao (PolyU: Hong Kong Polytechnic University)H-Index: 6
#2Jun Xia Xie (Qingdao University)H-Index: 3
Last. Wen Fang Chen (Qingdao University)H-Index: 11
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Abstract Genistein, an isoflavone naturally found in soy products, displays estrogenic properties. Our previous study clearly demonstrated that genistein can activate the insulin-like growth factor-I receptor (IGF-IR) signaling pathway in human breast cancer MCF-7 cells. The present study aims to test the hypothesis that the IGF-I receptor signaling pathway is involved in the neuroprotective effects of genistein in neuroblastoma SK-N-SH cells. Our results revealed that pretreatment with genistei...
21 CitationsSource
#1Wen Fang Chen (PolyU: Hong Kong Polytechnic University)H-Index: 11
#2Sao-Keng Mok (PolyU: Hong Kong Polytechnic University)H-Index: 2
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
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Flavonoids are the active components of Herba epimedii (HEP), a commonly used herb for the management of osteoporosis in China over the centuries. The present study aims to characterise the in vivo effects of its total flavonoid (TF) fraction on bone properties and mineral metabolism as well as to study the mechanism involved in achieving its protective effects against ovariectomy (OVX)-induced bone loss. TF suppressed OVX-induced increase in urinary Ca excretion as well as loss of bone mass and...
34 CitationsSource
#1Quan Gui Gao (PolyU: Hong Kong Polytechnic University)H-Index: 6
#2Wen Fang Chen (PolyU: Hong Kong Polytechnic University)H-Index: 11
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
view all 4 authors...
Our previous studies have demonstrated that ginsenoside Rg1 is a novel class of potent phytoestrogen and can mimic the action of estradiol in stimulation of MCF-7 cell growth by the crosstalk between insulin-like growth factor-I receptor (IGF-IR)-dependent pathway and estrogen receptor (ER)-dependent pathway. The present study was designed to investigate the neuroprotective effects of ginsenoside Rg1 against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in human neuroblastoma SK-N-SH cells an...
73 CitationsSource
#1Wai-Sum Lau (PolyU: Hong Kong Polytechnic University)H-Index: 3
#2Wen Fang Chen (PolyU: Hong Kong Polytechnic University)H-Index: 11
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
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Background and purpose: The present study was designed to determine how ginsenoside Rg1, an active ingredient in ginseng root, exerts its oestrogenic effects. We hypothesize that Rg1 may exert oestrogen-like actions in MCF-7 cells by activating the mitogen-activated protein kinase (MAPK) pathway in a ligand-independent manner. Experimental approach: MCF-7 cells were co-incubated with the MAPK inhibitor PD98059 to determine whether the stimulant effects of Rg1 on cell proliferation, the induction...
62 CitationsSource
#1Li-Xing Liu (Qingdao University)H-Index: 1
#2Wen Fang Chen (Qingdao University)H-Index: 11
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
view all 4 authors...
Emerging evidence suggests beneficial effects of estrogen and estrogen-like chemicals on neurodegenerative diseases, especially Parkinson's disease (PD). Genistein, an isoflavone naturally found in soy products, displays estrogenic properties. The present study aims to investigate the neuroprotective effects of genistein on dopaminergic neurons in ovariectomized (OVX), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model mice. MPTP significantly decreased the levels of dopamine (...
118 CitationsSource
#1Wen Fang Chen (Qingdao University)H-Index: 11
#2Quan Gui Gao (Qingdao University)H-Index: 6
Last. Man Sau Wong (PolyU: Hong Kong Polytechnic University)H-Index: 36
view all 3 authors...
Our previous studies have shown that genistein can enhance the insulin-like growth factor (IGF)-1 receptor signalling pathway via an oestrogen receptor (ER) in human breast cancer MCF-7 cells. The present study aims to investigate how genistein regulates IGF-1 receptor expression in human MCF-7 cells. Genistein at 1mM stimulated the growth of MCF-7 cells and this effect could be completely blocked by the IGF-1 receptor antagonist JB-1, suggesting that IGF-1 receptor is essential for mediating th...
20 CitationsSource
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