Qinghua Deng
Inner Mongolia University for Nationalities
Internal medicineMicrosomal triglyceride transfer proteinEndocrinologyChemistryPeroxisome proliferator-activated receptorSuperoxide dismutaseOxidative stressHepatocyteKetosisAMP-activated protein kinasePTENMalondialdehydeGlutathione peroxidaseFatty liverTensinLipoproteinAMPKInsulin-like growth factorCarbohydrate-responsive element-binding proteinLipid oxidationLDL receptorLipid metabolismBiochemistryApolipoprotein EApolipoprotein BVery low-density lipoproteinBiology
13Publications
8H-index
294Citations
Publications 13
Newest
#1Qinghua Deng (IMUN: Inner Mongolia University for Nationalities)H-Index: 8
#2Dehui Ma (IMUN: Inner Mongolia University for Nationalities)H-Index: 1
Last. Guowen Liu (IMUN: Inner Mongolia University for Nationalities)H-Index: 27
view all 6 authors...
: Dairy cows with fatty liver or ketosis display decreased insulin sensitivity and defects in the insulin receptor substrate (IRS)/PI3K/AKT signaling pathway. Phosphatase and tensin homolog (PTEN) is a well-known tumor suppressor and also a negative regulator of insulin signaling and peripheral insulin sensitivity. We investigated the hypothesis that PTEN may affect the insulin pathway-mediated hepatic glucose and lipid metabolism in dairy cows. Adenovirus vectors that over-express and silence P...
4 CitationsSource
#1Xiaoxia Shi (JLU: Jilin University)H-Index: 8
#2Dangdang Li (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
Abstract Dairy cows with fatty liver or ketosis exhibit hyperketonemia, oxidative stress, and a low rate of very low density lipoprotein (VLDL) assembly, and there may be a potential link among these characteristics. Therefore, the objective of this study was to determine the effect of acetoacetic acid (AcAc) on the assembly of VLDL in cow hepatocytes. Cultured cow hepatocytes were treated with different concentrations of AcAc with or without N-acetylcysteine (NAC, an antioxidant). AcAc treatmen...
9 CitationsSource
Summary β-Hydroxybutyricacid (BHBA) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low-density lipoprotein (VLDL) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA. We found that BHBA treatment upregulated the mRNA and ...
Source
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Yuan Guan (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
Abstract Fatty liver is a major metabolic disorder of dairy cows. One important reason is that hepatic very low-density lipoproteins (VLDL) assembly was significant decreased in dairy cows with fatty liver. In addition, the impairment of insulin-like growth factor (IGF)-1 synthesis was involved in the development of fatty liver. Therefore, the objective of this study was to investigate the effects of IGF-1 on the VLDL assembly in cow hepatocytes. In this study, cow hepatocytes were cultured and ...
1 CitationsSource
β-hydroxybutyric acid (BHBA), an important metabolite in β-oxidation, is involved in the development of ketosis in dairy cows. It is known that AMP-activated protein kinase (AMPK) signaling pathway plays an important role in the regulation of lipid metabolism in hepatocytes. In the present study, bovine hepatocytes were treated with BHBA at variable concontrations and Compound C (Cpd C, an AMPK inhibitor) to investigate the effects of BHBA on the AMPK signaling pathway. The results showed that w...
21 CitationsSource
Abstract Non-esterified fatty acids (NEFAs) are important induction factors of inflammatory responses in some metabolic diseases. High plasma levels of NEFAs and oxidative stress exist in the dairy cows with ketosis. The aim of this study was to investigate whether high levels of NEFAs can induce inflammatory response and the specific molecular mechanism in the hepatocytes of dairy cow. In vitro , primary cultured bovine hepatocytes were treated with different concentrations of NEFAs, PDTC (an N...
57 CitationsSource
Abstract The natural incidence of fatty liver in ruminants is significantly higher than in monogastric animals. Fatty liver is associated with sterol regulatory element-binding protein 1c (SREBP-1c). The aim of this study was to investigate the regulatory network effects of SREBP-1c on the lipid metabolic genes involved in fatty acid uptake, activation, oxidation, synthesis, and very low-density lipoprotein (VLDL) assembly in bovine hepatocytes. In vitro , bovine hepatocytes were transfected wit...
59 CitationsSource
#1Qinghua Deng (JLU: Jilin University)H-Index: 8
#2Xinwei LiH-Index: 23
Last. Xiaobing LiH-Index: 22
view all 13 authors...
Background: Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows. Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor that regulates lipid synthesis by regulating the expression of lipid metabolism genes. Methods: In this study, we reduced the expression of SREBP-1c by adenovirus-mediated SREBP-1c with a low expression vector (AD-GFP-SREBP-1c) to study the effects of SREBP-1c on lipid deposits in bovine...
18 CitationsSource
Background/Aims: s-hydroxybutyrate (BHBA) is the major component of ketone bodies in ketosis. Dairy cows with ketosis often undergo oxidative stress. BHBA is related to the inflammation involved in other diseases of dairy cattle. However, whether BHBA can induce inflammatory injury in dairy cow hepatocytes and the potential mechanism of this induction are not clear. The NF-κB pathway plays a vital role in the inflammatory response. Methods: Therefore, this study evaluated the oxidative stress, p...
59 CitationsSource
#1Hui Chen (JLU: Jilin University)H-Index: 93
#2Liang Zhang (JLU: Jilin University)H-Index: 6
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 16 authors...
Abstract Adiponectin (Ad) plays a crucial role in hepatic lipid metabolism. However, the regulating mechanism of hepatic lipid metabolism by Ad in dairy cows is unclear. Hepatocytes from a newborn female calf were cultured in vitro and treated with different concentrations of Ad and BML-275 (an AMPKα inhibitor). The results showed that Ad significantly increased the expression of two Ad receptors. Furthermore, the phosphorylation and activity of AMPKα, as well as the expression levels and transc...
50 CitationsSource