Yajing Liu
China Agricultural University
Internal medicineEndocrinologyAnimal scienceCellulaseChemistryConcanavalin AXylanaseMannoseGalactomycesGeotrichumCorn stoverValeratePeanut oilIleumSalineCecumProstaglandin E2GalactoseCorn oilStachyoseLinseed oilLipopolysaccharideCorncobLipaseRapeseedSoybean oilXylobioseFish oilRaffinoseLactobacillusXyloseWeight gainTrypsinPrevotellaSunflower oilAspergillus oryzaePichia pastorisFood scienceChromatographyAnimal feedBiochemistryBiologyHydrolysisSomatotropic cell
5Publications
2H-index
72Citations
Publications 5
Newest
#1Yajing Liu (CAU: China Agricultural University)H-Index: 2
#2Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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A study was carried out to investigate the characterization of a novel Aspergillus sulphureus JCM01963 xylanase (AS-xyn10A) with a carbohydrate binding module (CBM) and its application in degrading alkali pretreated corncob, rapeseed meal and corn stover alone and in combination with a commercial cellulase. In this study, the 3D structure of AS-xyn10A, which contained a CBM at C-terminal. AS-xyn10A and its CBM-truncated variant (AS-xyn10A-dC) was codon-optimized and over-expressed in Komagaella ...
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#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Xu Yang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
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Abstract The α-galactosidase gene (galC) was cloned from Aspergillus oryzae YZ1 and expressed in Pichia pastoris. The galC (2319 bp) containing two introns encoded a protein of 726 amino acids. The activity of the α-galactosidase (GalC) increased 1-fold after coding sequence optimization. Purified GalC exhibited a single protein band (100 kDa) in SDS-PAGE. The optimum pH and temperature of GalC were pH 4.66 and 50 °C, respectively. Like many GH36 family α-galactosidases, GalC displayed its activ...
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#1Wang Jian (CAU: China Agricultural University)H-Index: 2
#1Jian Wang (CAU: China Agricultural University)H-Index: 1
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 1
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An acidic thermostable xylanase (AT-xynA) which was stable at low pH and high temperature was considered to have great potential in animal feed. For large-scale production, AT-xynA activity was enhanced about 1-fold in Pichia pastoris by constructing a double-copy expression strain in this study. Furthermore, impacts of different AT-xynA levels on growth performance, nutrient digestibility, short-chain fatty acids, and bacterial community in weaned piglets were determined. Compared with the cont...
3 CitationsSource
#1Jian Wang (CAU: China Agricultural University)H-Index: 1
#2Yajing Liu (CAU: China Agricultural University)H-Index: 2
Last. Yunhe Cao (CAU: China Agricultural University)H-Index: 7
view all 5 authors...
A Galactomyces geotrichum strain with lipolytic activity was isolated and identified by the analysis of internal transcribed spacer (ITS) sequence of 18 s rDNA. Full-length lipase gene of this stain is composed of 1692 base pairs (bp) without intron, which encodes a 563-amino-acid protein. A catalytic triad (Ser217–Glu354–His463) was found by constructing the three-dimensional structure of the lipase. In shake flasks, the lipase (LIP) catalytic activity in the supernatant of the recombinant Pich...
1 CitationsSource
#1Yajing Liu (CAU: China Agricultural University)H-Index: 2
#1Y. L. Liu (CAU: China Agricultural University)H-Index: 1
Last. J. A. Carroll (ARS: Agricultural Research Service)H-Index: 24
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Seventy-two crossbred pigs (7.58 ± 0.30 kg BW) weaned at 28 ± 3 d of age were used to investigate the effects of fish oil supplementation on pig performance and on immunological, adrenal, and somatotropic responses following an Escherichia coli lipopolysaccharide (LPS) challenge in a 2 x 2 factorial design. The main factors consisted of diet (7% corn oil [CO] or 7% fish oil [FO]) and immunological challenge (LPS or saline). On d 14 and 21, pigs were injected intraperitoneally with either 200 μg/...
98 CitationsSource